How much is enough? Minimum sampling intensity required to capture extant genetic diversity in ex situ seed collections: examples from the endangered plant Sibara filifolia (Brassicaceae)

McGlaughlin, Mitchell E.; Riley, Lynn; Brandsrud, Michelle; Arcibal, Erica; Helenurm, Mark K.; Helenurm, Kaius

doi:10.1007/s10592-014-0655-3

Cited by 24 publications

(17 citation statements)

References 40 publications

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“…Much progress has been made in understanding the sampling strategies for living conservation collections, mostly for seed storage (Schoen and Brown 2001;Guerrant et al 2004McGlaughlin et al 2015) and also with regard to sampling breadth (Larkin et al 2016). This new understanding for botanic garden work has a basis in the agricultural sciences (Frankel 1984;Gale and Lawrence 1984;Brown 1989) which could be more vigorously applied (Heywood 2009).…”

Section: Sampling Strategies For Genetic Capturementioning

confidence: 99%

Will the same ex situ protocols give similar results for closely related species?

Griffith

Calonje

Meerow³

et al. 2017

Biodivers Conserv

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Conservation of imperiled plant species often requires ex situ (offsite) living collections. Protocols for developing these collections most often emphasize sampling depth, but little is known about the genetics of such collections. This study compares how well a single collecting protocol can capture the diversity in wild populations of two closely related species. We selected two exemplar species, bay rush (Zamia lucayana) and sinkhole cycad (Zamia decumbens), based on similarities and differences that allow for rigorous comparison, including geographic isolation and reproductive factors. For each species, we compared in situ plants to ex situ plants via the same panel of 10 microsatellite markers. Genetic distance analysis shows high fidelity of the ex situ collections to their in situ source populations and sub-populations. Structured resampling of allele capture from the in situ populations by the ex situ collections shows that allele capture increases as number of ex situ plants maintained increases, but with a diminishing rate of increase. Difference in the rate of allele capture between the two species was significant at the 123Biodivers Conserv (2017) 26:2951-2966 DOI 10.1007/s10531-017-1400-2 a = 0.1 level, (p = 0.097) but not at the a = 0.05 level. At larger collection sizes, the difference in rate of allele capture showed a high practical significance (d = 5.41). These data illustrate that a unified collecting protocol can achieve similar allele capture among related species, but also that geographic and reproductive factors can influence the rate of allele capture.

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Section: Sampling Strategies For Genetic Capturementioning

confidence: 99%

Will the same ex situ protocols give similar results for closely related species?

Griffith

Calonje

Meerow³

et al. 2017

Biodivers Conserv

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“…A number of studies have examined how many trees and populations to sample for ex situ conservation, both empirically [34,[36][37][38], and theoretically [33,[63][64][65]. These studies have shown that appropriate sampling strategies depend on the species and the situation.…”

Section: Discussionmentioning

confidence: 99%

“…Ex situ seed collections protocols suggest that in order to maximize the local diversity collected, you need to sample a minimum of 50 unrelated maternal trees per population, and repeat for as many populations as possible [33]. However, the appropriate design of a conservation collection depends on various aspects of the target species, including population size, reproductive biology, recent population history, and connectivity among populations [34][35][36][37][38][39]. Given that it is nearly impossible to preserve all genetic material, determining the optimum number and spatial arrangement of treated trees will be important in efforts to conserve the majority of local genetic variation.…”

mentioning

confidence: 99%

Optimizing Conservation Strategies for a Threatened Tree Species: In Situ Conservation of White Ash (Fraxinus americana L.) Genetic Diversity through Insecticide Treatment

Flower

Fant²,

Hoban

et al. 2018

Forests

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Forest resources face numerous threats that require costly management. Hence, there is an increasing need for data-informed strategies to guide conservation practices. The introduction of the emerald ash borer to North America has caused rapid declines in ash populations (Fraxinus spp. L.). Natural resource managers are faced with a choice of either allowing ash trees to die, risking forest degradation and reduced functional resilience, or investing in conserving trees to preserve ecosystem structure and standing genetic diversity. The information needed to guide these decisions is not always readily available. Therefore, to address this concern, we used eight microsatellites to genotype 352 white ash trees (Fraxinus americana L.) across 17 populations in the Allegheny National Forest; a subset of individuals sampled are part of an insecticide treatment regimen. Genetic diversity (number of alleles and He) was equivalent in treated and untreated trees, with little evidence of differentiation or inbreeding, suggesting current insecticidal treatment is conserving local, neutral genetic diversity. Using simulations, we demonstrated that best practice is treating more populations rather than more trees in fewer populations. Furthermore, through genetic screening, conservation practitioners can select highly diverse and unique populations to maximize diversity and reduce expenditures (by up to 21%). These findings will help practitioners develop cost-effective strategies to conserve genetic diversity.

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“…The admixture model with correlated allele frequencies was used, with a burn‐in of 100,000 and 1,000,000 Markov chain Monte Carlo (MCMC) repetitions and 10 iterations per K ( K = 1–10). The method of Evanno, Regnaut, and Goudet () was used to determine the best fit value, as implemented in STRUCTURE HAVESTER (Earl & Vonholdt, ; McGlaughlin et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…The method of Evanno, Regnaut, and Goudet (2005) was used to determine the best fit value, as implemented in STRUCTURE HAVESTER (Earl & Vonholdt, 2012;McGlaughlin et al, 2015). Pritchard et al (2000) presented a formal Bayesian test to assess whether any individual within the samples was a migrant to this group or a resident.…”

Section: Detection Of Migrantsmentioning

confidence: 99%

Dispersal of Amur tiger from spatial distribution and genetics within the eastern Changbai mountain of China

Yao

Kostyria

et al. 2019

Ecology and Evolution

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Population dispersal and migration often indicate an expanded habitat and reduced inbreeding probability, and to some extend reflects improvement in the condition of the population. The Amur tiger population in the northern region of the Changbai mountain in China mostly distributes along the Sino–Russian border, next to the population in southwest Primorye in Russia. The successful dispersal westward and transboundary movement are crucial for the persistence of the Amur tiger in this area. This study explored the spatial dispersal of the population, transboundary migration, and the genetic condition of the Amur tiger population within the northern Changbai mountain in China, using occurrence data and fecal samples. Our results from 2003 to 2016 showed that the Amur tiger population in this area was spreading westward at a speed of 12.83 ± 4.41 km every three years. Genetic diversity of the Amur tiger populations in southwest Primorye was slightly different than the population in our study area, and the potential individual migration rate between these two populations was shown to be about 13.04%. Furthermore, the relationships between genetic distances and spatial distances indicated the existence of serious limitations to the dispersal of the Amur tiger in China. This study provided important information about spatial dispersal, transboundary migration, and the genetic diversity of Amur tigers in China, showed the urgent need for Amur tiger habitat restoration, and suggested some important conservation measures, such as corridor construction to eliminate dispersal barriers and joint international conservation to promote trans‐boundary movement.

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How much is enough? Minimum sampling intensity required to capture extant genetic diversity in ex situ seed collections: examples from the endangered plant Sibara filifolia (Brassicaceae)

Cited by 24 publications

References 40 publications

Will the same ex situ protocols give similar results for closely related species?

Will the same ex situ protocols give similar results for closely related species?

Optimizing Conservation Strategies for a Threatened Tree Species: In Situ Conservation of White Ash (Fraxinus americana L.) Genetic Diversity through Insecticide Treatment

Dispersal of Amur tiger from spatial distribution and genetics within the eastern Changbai mountain of China

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