How nascent phagosomes mature to become phagolysosomes

Fairn, Gregory D.; Grinstein, Sergio

doi:10.1016/j.it.2012.03.003

Cited by 251 publications

(253 citation statements)

References 78 publications

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“…Interestingly, this recycling machinery is also dependent on PI3P expression for docking onto intracellular structures 37 , further supporting our notion of PI3ks as important role player in this context. Not all engulfed material is destined for degradation and subsequently need to be recycled by the recycling endosomes and SNX mentioned above.…”

Section: Recognitionsupporting

confidence: 81%

“…Recycling endosomes, partly identified by the Rab11 superfamily, is responsible for pinching off reusable cargo during the nascent phagosomal stage 38 . One of three Rab11 isoforms, Rab11a, is expressed on the nascent phagosome itself and facilitates phagosome fusion with early endosomes and Golgi-derived vesicles 37 . Rab11a also has a crucial role in pro-inflammatory signalling as it delivers TLR4, responsible for detection of lipopolysaccharide such as E.coli LPS, to maturing phagosomes 39 .…”

Section: Recognitionmentioning

confidence: 99%

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Development of a transendothelial shuttle by macrophage modification

Visser

Smith

2017

J Tissue Eng Regen Med

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i Declaration By submitting this thesis electronically, I declare that the entirety of the work contained therein is my own, original work, that I am the sole author thereof (save to the extent explicitly otherwise stated), that reproduction and publication thereof by Stellenbosch University will not infringe any third party rights and that I have not previously in its entirety or in part submitted it for obtaining any qualification. Copyright © 2016 Stellenbosch UniversityAll rights reserved Stellenbosch University https://scholar.sun.ac.za ii Abstract Background: Targeted stem cell delivery via macrophage modification is a novel and

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Section: Recognitionsupporting

confidence: 81%

Section: Recognitionmentioning

confidence: 99%

Development of a transendothelial shuttle by macrophage modification

Visser

Smith

2017

J Tissue Eng Regen Med

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“…Using VACV as ligand, we found that the process of macropinosome maturation shares many features with the maturation of endosomes 3, phagosomes 74 and autophagosomes 75. Our findings indicated that maturation was essential for the infectivity of VACV MVs and EVs (Figure 6).…”

Section: Discussionmentioning

confidence: 74%

Vaccinia Virus Infection Requires Maturation of Macropinosomes

Rizopoulos

Balistreri

Kilcher

et al. 2015

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The prototypic poxvirus, vaccinia virus (VACV), occurs in two infectious forms, mature virions (MVs) and extracellular virions (EVs). Both enter HeLa cells by inducing macropinocytic uptake. Using confocal microscopy, live‐cell imaging, targeted RNAi screening and perturbants of endosome maturation, we analyzed the properties and maturation pathway of the macropinocytic vacuoles containing VACV MVs in HeLa cells. The vacuoles first acquired markers of early endosomes [Rab5, early endosome antigen 1 and phosphatidylinositol(3)P]. Prior to release of virus cores into the cytoplasm, they contained markers of late endosomes and lysosomes (Rab7a, lysosome‐associated membrane protein 1 and sorting nexin 3). RNAi screening of endocytic cell factors emphasized the importance of late compartments for VACV infection. Follow‐up perturbation analysis showed that infection required Rab7a and PIKfyve, confirming that VACV is a late‐penetrating virus dependent on macropinosome maturation. VACV EV infection was inhibited by depletion of many of the same factors, indicating that both infectious particle forms share the need for late vacuolar conditions for penetration.

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“…These eventual antiseptic properties are gained following phagosomal maturation, which entails a succession of precisely choreographed membrane fusion and fission events to remodel the phagosomal membrane and contents, culminating in the biogenesis of phagolysosomes. 35 As with the endocytic pathway, the phagosome undergoes early, late and lysosome-fusion stages of maturation. The conversion between phagosomal stages is delicately coordinated by the Rab family GTPases, which comprise over 60 members in mammals.…”

Section: Function Of the Ubiquitin System In Phagosomal Maturation Dumentioning

confidence: 99%

The ubiquitin system: a critical regulator of innate immunity and pathogen–host interactions

Chai

Liu

2016

Cell Mol Immunol

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The ubiquitin system comprises enzymes that are responsible for ubiquitination and deubiquitination, as well as ubiquitin receptors that are capable of recognizing and deciphering the ubiquitin code, which act in coordination to regulate almost all host cellular processes, including host-pathogen interactions. In response to pathogen infection, the host innate immune system launches an array of distinct antimicrobial activities encompassing inflammatory signaling, phagosomal maturation, autophagy and apoptosis, all of which are fine-tuned by the ubiquitin system to eradicate the invading pathogens and to reduce concomitant host damage. By contrast, pathogens have evolved a cohort of exquisite strategies to evade host innate immunity by usurping the ubiquitin system for their own benefits. Here, we present recent advances regarding the ubiquitin system-mediated modulation of host-pathogen interplay, with a specific focus on host innate immune defenses and bacterial pathogen immune evasion.

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How nascent phagosomes mature to become phagolysosomes

Cited by 251 publications

References 78 publications

Development of a transendothelial shuttle by macrophage modification

Development of a transendothelial shuttle by macrophage modification

Vaccinia Virus Infection Requires Maturation of Macropinosomes

The ubiquitin system: a critical regulator of innate immunity and pathogen–host interactions

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