How pH, Temperature, and Time of Incubation Affect False-Positive Responses and Uncertainty of the LAL Gel-Clot Test

Lourenço, Felipe Rebello; Botelho, Túlia de Souza; Pinto, Terezinha de Jesus Andreoli

doi:10.5731/pdajpst.2012.00887

Cited by 27 publications

(16 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…7 However, the LAL assay is very sensitive and highly susceptible to changes in temperature and pH, as well as potential interference from other carbohydrate derivatives, such as b-glucans. 8 Therefore, numerous efforts have been devoted to the development of alternative assays for LPSs. Owing to the highly negative charges of the LPS endowed with its phosphate and carboxylic groups, the utilization of the electrostatic interaction between the LPS and positively charged probes to develop sensors for LPSs has attracted much interest.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric detection of lipopolysaccharides based on a lipopolysaccharide-binding peptide and AuNPs

Lei

Qiao

et al. 2016

Anal. Methods

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Colorimetric detection of lipopolysaccharides based on a lipopolysaccharide-binding peptide and AuNPs

Lei

Qiao

et al. 2016

Anal. Methods

View full text Add to dashboard Cite

show abstract

“…Enzymatic limulus amoebocyte lysate assay is commonly used to detect LPS, but this method is susceptible to variations in experimental conditions, particularly pH and temperature, and requires complicated sample preparation and testing procedures. [16][17][18] To address these drawbacks, scholars have developed alternative methods for LPS detection by using various signals, such as colorimetric, [19][20][21][22] surface-enhanced Raman scattering, 23 uorescence, 24 and electrochemical signals. [25][26][27] These methods exhibit high sensitivity and accuracy.…”

Section: 15mentioning

confidence: 99%

An aptamer-based fluorescence probe for facile detection of lipopolysaccharide in drinks

et al. 2017

View full text Add to dashboard Cite

Bacterial toxin contamination is a serious food safety issue. Rapid, facile detection of bacterial toxins in food is crucial to control their harmful effects on human health. In this study, a facile, selective, and cost-effective method with a 6-carboxy-X-rhodamine labeled lipopolysaccharide binding aptamer (ROX-LBA)/GO fluorescent probe was developed based on the specific recognition characteristics of aptamers and the super fluorescence quenching efficiency of graphene oxide (GO) and used for the detection of lipopolysaccharides (LPS). ROX-LBA is adsorbed on the GO surface because of its strong p-p stacking interactions in the flexible single-stranded state, and fluorescence is quenched by GO via Förster resonance energy transfer (FRET). After adding LPS, the aptamer binds to LPS and forms a LPS/ROX-LBA complex, which exhibits weak binding affinity with GO. ROX-LBA cannot effectively combine with GO, and fluorescence is restored. The concentration of LPS can be quantitatively analyzed by observing fluorescence changes in the sensing system. The fluorescence intensity proportionally increases with LPS concentration (25-1600 ng mL À1 ), and the detection limit is 15.7 ng mL À1 . Hence, the proposed method provides an alternative strategy for the specific detection of endotoxin in drink samples (e.g., apple juice and beer) and could be widely applied to quantify various bacterial toxins by replacing the aptamer sequences.

show abstract

“…However, the currently used enzymatic Limulus amebocyte lysate (LAL) assay is highly susceptible to changes in temperature and pH, 255 various interference factors (either inhibiting or enhancing) in the samples, [256][257][258][259] and requires cumbersome sample preparation, storage and controlled experimental condition. 255,260 Herein we developed a simple colorimetric biosensor for LPS utilizing graphene oxide and synthetic peptide. The principle of this biosensor is shown in Figure 7.5.…”

Section: Chapter 7: Collaborative Projects On Plasmonics and Biosensorsmentioning

confidence: 99%

Localized surface plasmon resonance (LSPR) based biosensors

Chen¹

View full text Add to dashboard Cite

Biosensors provide a quick and cheap alternative way of characterization and analysis as compared to standard analytical methods such as spectrometry, chromatography, biochemical or microbiological techniques. In the past few decades, biosensors have been quickly developed both in fundamental research and applications in the areas of medical diagnosis, pharmaceutics and environmental monitoring, as well as food, forensic, sports and defense and military science etc. Many different sensing platforms including the enzymelinked immunosorbent assay (ELISA), surface plasmon resonance (SPR) and electrochemical sensor have been developed and found practical applications in medical diagnosis, environmental monitoring, detection of explosives etc. 1,2 Most of these sophisticated sensing platforms are available at test centers, laboratories and hospitals etc. Moreover, these biosensors generally require professional training on operation. In this thesis, I developed some biosensor that is suitable for field test. These sensors are generally robust enough to stand for harsh environmental conditions, simple to operate and require minimal instrumentations. Localized surface plasmon resonance (LSPR) of gold nanoparticles has attracted tremendous interest in biosensing, due to its unique optical properties. The frequency and strength of the surface plasmon resonance of noble metal nanoparticles is affected by many factors, including the size, shape, inter-particle distance and surrounding medium etc. The resonance frequency and strength of the local electromagnetic field can be used to monitor the molecular interactions (bindings) occurs on (near) the surface of particle. In addition, graphene, a new Firstly, I would like to thank Centre for Biomimetic Sensor Science and School of Materials Science and Engineering for providing me this opportunity to work on this excellent project. I would like to thank my supervisor Prof. Bo Liedberg for his guidance and advice for the research work during my PhD study. From him I learned quite a lot of knowledge, skills and the integrity as a researcher. I would also like to express my great gratitude to Prof. Zhang Hua in MSE and Prof. Daniel Aili at Linköping University, Sweden who has given me much guidance and training on graphene oxide and peptide related work, respectively. Through them, I have gained valuable insights in the research related to nano-bio interface.

show abstract

How pH, Temperature, and Time of Incubation Affect False-Positive Responses and Uncertainty of the LAL Gel-Clot Test

Cited by 27 publications

References 5 publications

Colorimetric detection of lipopolysaccharides based on a lipopolysaccharide-binding peptide and AuNPs

Colorimetric detection of lipopolysaccharides based on a lipopolysaccharide-binding peptide and AuNPs

An aptamer-based fluorescence probe for facile detection of lipopolysaccharide in drinks

Localized surface plasmon resonance (LSPR) based biosensors

Contact Info

Product

Resources

About