How Reliable Are Hematological Parameters in Predicting Uncomplicated<i>Plasmodium falciparum</i>Malaria in an Endemic Region?

Muwonge, Haruna; Kikomeko, Sharif; Sembajjwe, Larry Fred; Seguya, Abdul; Namugwanya, Christine

doi:10.1155/2013/673798

Cited by 50 publications

(56 citation statements)

References 32 publications

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“…7 This study showed an increase in monocyte count in malaria patients with a mean of 9.4 (5.4)%. This result was similar to Muwonge et al 9 who showed that an average count of monocytes in patients with malaria amounted to 10.89 (6.23)%. Monocytosis was one of hematological changes consistent in malaria.…”

Section: Resultssupporting

confidence: 91%

“…Hematological changes were found in patients with malaria such as normocytic normochrome anemia and thrombocytopenia. 12 Muwonge et al 9 in Uganda reported an increased levels of monocytes to 61 malaria patients of about 10.89 (6.23). However, studies of Chandra & Chandra 13 in India during August 2008 until August 2010 reported that thrombocytopenia (platelet count <150,000/ mm 3 ) was significant in acute malaria compared to controls with a sensitivity of 87.2% and a specificity of 65%.…”

Section: Introductionmentioning

confidence: 97%

“…9,10 Laboratory and clinical findings of malaria often give results that are not specific. 9 Resulting in difficulties in establishing the correct diagnosis, so that the provision of antimalarial drugs become incorrect. 2 World Health Organization recommended microscopic examination and Rapid Diagnostic Test (RDT) in patients suspected of malaria such are febrile illnesses patients who live in endemic areas of malaria, or with a history of travel to endemic areas of malaria.…”

Section: Introductionmentioning

confidence: 99%

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DIAGNOSTIC TEST OF HEMATOLOGY PARAMETER IN PATIENTs SUSPECT OF MALARIA

Ferawati¹,

Maani²,

Rofinda³

et al. 2018

Indonesian J. Clin. Pathol. Med. Lab.

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Malaria masih merupakan masalah kesehatan di daerah tropis dan sub tropis terutama Asia dan Afrika dengan angka kesakitan dankematian yang tinggi. Parasit masuk ke dalam darah selain menimbulkan gejala klinis berupa demam, juga diduga memicu terjadinyaperubahan hematologi antara lain monositosis dan trombositopenia. Penelitian ini bertujuan mengetahui uji diagnostik tolok ukurhematologi di pasien terduga malaria. Penelitian uji diagnostik potong lintang ini dilakukan terhadap 60 orang pasien terduga malariayang memenuhi patokan kesertan dan nonkesertaan masa waktu Juli 2015 sampai Maret 2016 di Instalasi Laboratorium Sentral RSUP.Dr. M. Djamil Padang, Rumah Sakit Tingkat III Reksodiwiryo Padang, Puskesmas Barung Belantai Kabupaten Pesisir Selatan, RumahSakit Hanafie Kabupaten Bungo, Rumah Sakit Sultan Thaha Saifuddin dan Puskesmas Rimbo Bujang Kabupaten Tebo. Tolok ukur yangdiperiksa selain mikroskopis malaria adalah hitung monosit dan trombosit. Analisis statistik menggunakan piranti lunak dan Tabel2×2. Kepekaan dan kekhasan demam, bertempat tinggal atau ditemukan riwayat perjalanan di daerah endemis malaria serta hitungmonosit >8% dan hitung trombosit <150.000/mm3 dibandingkan pemeriksaan mikroskopis pada penelitian ini berturut-turut adalah81,6% dan 81,8%. Nilai duga positif, nilai duga negatif, rasio kemungkinan positif dan rasio kemungkinan negatif pada penelitian iniberturut-turut adalah 88,6%, 72%, 4,5 dan 0,2. Penelitian ini mendapatkan kepekaan dan kekhasan demam, bertempat tinggal atauditemukan riwayat perjalanan di daerah endemis malaria serta hitung monosit >8% dan hitung trombosit <150.000/mm3 yang tinggidibandingkan pemeriksaan mikroskopis di pasien malaria.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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DIAGNOSTIC TEST OF HEMATOLOGY PARAMETER IN PATIENTs SUSPECT OF MALARIA

Ferawati¹,

Maani²,

Rofinda³

et al. 2018

Indonesian J. Clin. Pathol. Med. Lab.

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“…Similar results were reported in patients with uncomplicated malaria where the authors indicated that, uncomplicated malaria was associated with milder biochemical alteration and haemolysis as opposed to complicated/severe malaria [26]. A study done at ECWA Community Health Centre, Bukuru, Jos in Nigeria however showed significant reduction of the haematocrit of malaria samples compared to healthy controls [22].…”

Section: Discussionsupporting

confidence: 70%

Proteomic analysis of microparticles isolated from malaria positive blood samples

et al. 2016

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BackgroundMalaria continues to be a great public health concern due to the significant mortality and morbidity associated with the disease especially in developing countries. Microparticles (MPs), also called plasma membrane derived extracellular vesicles (PMEVs) are subcellular structures that are generated when they bud off the plasma membrane. They can be found in healthy individuals but the numbers tend to increase in pathological conditions including malaria. Although, various studies have been carried out on the protein content of specific cellular derived MPs, there seems to be paucity of information on the protein content of circulating MPs in malaria and their association with the various signs and symptoms of the disease. The aim of this study was therefore to carry out proteomic analyses of MPs isolated from malaria positive samples and compare them with proteins of MPs from malaria parasite culture supernatant and healthy controls in order to ascertain the role of MPs in malaria infection.MethodsPlasma samples were obtained from forty-three (43) malaria diagnosed patients (cases) and ten (10) healthy individuals (controls). Malaria parasite culture supernatant was obtained from our laboratory and MPs were isolated from them and confirmed using flow cytometry. 2D LC-MS was done to obtain their protein content. Resultant data were analyzed using SPSS Ver. 21.0 statistical software, Kruskal Wallis test and Spearman’s correlation coefficient r.ResultsIn all, 1806 proteins were isolated from the samples. The MPs from malaria positive samples recorded 1729 proteins, those from culture supernatant were 333 while the control samples recorded 234 proteins. The mean number of proteins in MPs of malaria positive samples was significantly higher than that in the control samples. Significantly, higher quantities of haemoglobin subunits were seen in MPs from malaria samples and culture supernatant compared to control samples.ConclusionA great number of proteins were observed to be carried in the microparticles (MPs) from malaria samples and culture supernatant compared to controls. The greater loss of haemoglobin from erythrocytes via MPs from malaria patients could serve as the initiation and progression of anaemia in P.falciparum infection. Also while some proteins were upregulated in circulating MPs in malaria samples, others were down regulated.

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“…Entry of P. falciparuminto erythrocytes usually leads to a marked increase in secretion of inflammatory cytokines (TNFα, IL-1, IL-10, and IFN γ ), endothelial cell activation (due to overexpression of cell adhesion molecules; ICAM-1, VCAM-1), activation of the coagulation cascade (due to platelet consumption and endothelial damage), and sequestration of parasitized RBCs (Haruna et al, 2013). Certain haematological changes which include low platelet count, haemoglobin concentration and hematocrit have been reported to be associated with malaria (Kumar and Shashirekha, 2006).…”

Section: Introductionmentioning

confidence: 99%

Determination of some haematological parameters in malaria infected subjects in Usmanu Danfodiyo University Teaching Hospital (UDUTH) Sokoto, Nigeria

Garba¹,

Danladi²,

Muhammad³

2015

Bayero J. Pure App. Sci.

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Since malaria parasites are able to attach to receptors on the red blood cell surface, it is expected that malaria parasites may have effect on some haematological parameters. Haematological parameters are often needed to assist in the management of malaria infection. The aim of this study is to assess the effects of malaria parasites on some haematological parameters in Sokoto Nigeria. To investigate the effects of malaria infection on red blood cells, white blood cells and platelets, 100 malaria positive adults (15-60 years) attending Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria were enrolled into the study for a period of seven months; April through October 2009. Malaria parasitaemia was determined microscopically by stained thick film, packed cell volume (PCV) by microhaematocrit method, while total white blood cell count (TWBC) and platelet count (PLC) by manual methods. The total of 100 malaria infected patients and 50 apparently healthy malaria non-infected students were compared against PCV, TWBC and PLC values using student's t-test. The results of PCV and PLC showed statistically significant decrease among malaria infected patients (p<0.05) while for TWBC there was statistically significant increase (p<0.05) among 18% of malaria infected subjects. When PCV, TWBC and PLC values of males and females infected with malaria parasite were compared, the PCV value showed statistically significant decrease in females (p<0.05), while TWBC and PLC values showed no statistically significant difference between males and females (p>0.05). These findings indicate that malaria infection has an impact on some haematological parameters, therefore, malaria infection in this community contributes to the severity of anaemia and thrombocytopenia.

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How Reliable Are Hematological Parameters in Predicting UncomplicatedPlasmodium falciparumMalaria in an Endemic Region?

Cited by 50 publications

References 32 publications

DIAGNOSTIC TEST OF HEMATOLOGY PARAMETER IN PATIENTs SUSPECT OF MALARIA

DIAGNOSTIC TEST OF HEMATOLOGY PARAMETER IN PATIENTs SUSPECT OF MALARIA

Proteomic analysis of microparticles isolated from malaria positive blood samples

Determination of some haematological parameters in malaria infected subjects in Usmanu Danfodiyo University Teaching Hospital (UDUTH) Sokoto, Nigeria

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