How the stomate got his pore: very long chain fatty acids and a structural cell wall protein sculpt the guard cell outer cuticular ledge

Hunt, Lee; Gray, Julie E.

doi:10.1111/nph.16843

Cited by 10 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These genes span 23 GO terms possibly representing decreased investment in fatty acid biosynthesis including very long chain fatty acids (VLCFAs) which are precursors for cellular membranes, wax and suberin, and also necessary for auxin transport affecting tissue patterning ( [60], electronic supplementary material, figure S8). This is intriguing since epidermal cell morphology, especially the extension of stomatal guard cell walls in the form of outer cuticular ledge, affects resistance to drought and is dependent on VLCFA deposition [61,62]. Relaxation from expensive anabolic processes in the leaf tissue allows diversion of resources towards growth in woody tissues or greater allocation to seed storage lipids.…”

Section: Discussionmentioning

confidence: 99%

Leaf gene expression trajectories during the growing season are consistent between sites and years in American beech

Sezen,

Shue,

Worthy

et al. 2024

Proc. R. Soc. B.

View full text Add to dashboard Cite

Transcriptomics provides a versatile tool for ecological monitoring. Here, through genome-guided profiling of transcripts mapping to 33 042 gene models, expression differences can be discerned among multi-year and seasonal leaf samples collected from American beech trees at two latitudinally separated sites. Despite a bottleneck due to post-Columbian deforestation, the single nucleotide polymorphism-based population genetic background analysis has yielded sufficient variation to account for differences between populations and among individuals. Our expression analyses during spring-summer and summer-autumn transitions for two consecutive years involved 4197 differentially expressed protein coding genes. Using Populus orthologues we reconstructed a protein-protein interactome representing leaf physiological states of trees during the seasonal transitions. Gene set enrichment analysis revealed gene ontology terms that highlight molecular functions and biological processes possibly influenced by abiotic forcings such as recovery from drought and response to excess precipitation. Further, based on 324 co-regulated transcripts, we focused on a subset of GO terms that could be putatively attributed to late spring phenological shifts. Our conservative results indicate that extended transcriptome-based monitoring of forests can capture diverse ranges of responses including air quality, chronic disease, as well as herbivore outbreaks that require activation and/or downregulation of genes collectively tuning reaction norms maintaining the survival of long living trees such as the American beech.

show abstract

Section: Discussionmentioning

confidence: 99%

Leaf gene expression trajectories during the growing season are consistent between sites and years in American beech

Sezen,

Shue,

Worthy

et al. 2024

Proc. R. Soc. B.

View full text Add to dashboard Cite

show abstract

“…However, it cannot be excluded that the cuticle composition changes are a secondary effect of stress signaling (Voxeur et al ., 2017; Chen et al ., 2022). The precise function of OCLs is still poorly characterized (Hunt & Gray, 2020); however, changes in OCL structure provide little explanation for additive water loss observed in double mutants of mur1 and guard cell signaling components (Fig. 5a).…”

Section: Discussionmentioning

confidence: 99%

Synthesis and import of GDP‐l‐fucose into the Golgi affect plant–water relations

Waszczak,

Yarmolinsky,

Leal Gavarrón

et al. 2023

New Phytologist

View full text Add to dashboard Cite

Summary Land plants evolved multiple adaptations to restrict transpiration. However, the underlying molecular mechanisms are not sufficiently understood. We used an ozone‐sensitivity forward genetics approach to identify Arabidopsis thaliana mutants impaired in gas exchange regulation. High water loss from detached leaves and impaired decrease of leaf conductance in response to multiple stomata‐closing stimuli were identified in a mutant of MURUS1 (MUR1), an enzyme required for GDP‐l‐fucose biosynthesis. High water loss observed in mur1 was independent from stomatal movements and instead could be linked to metabolic defects. Plants defective in import of GDP‐l‐Fuc into the Golgi apparatus phenocopied the high water loss of mur1 mutants, linking this phenotype to Golgi‐localized fucosylation events. However, impaired fucosylation of xyloglucan, N‐linked glycans, and arabinogalactan proteins did not explain the aberrant water loss of mur1 mutants. Partial reversion of mur1 water loss phenotype by borate supplementation and high water loss observed in boron uptake mutants link mur1 gas exchange phenotypes to pleiotropic consequences of l‐fucose and boron deficiency, which in turn affect mechanical and morphological properties of stomatal complexes and whole‐plant physiology. Our work emphasizes the impact of fucose metabolism and boron uptake on plant–water relations.

show abstract

“…S8). This is intriguing since epidermal cell morphology, especially the extension of stomatal guard cell walls in the form of outer cuticular ledge, affects resistance to drought and is dependent on VLCFA deposition [54, 55]. Relaxation from expensive anabolic processes in the leaf tissue allows diversion of resources towards growth in woody tissues or greater allocation to seed storage lipids.…”

Section: Discussionmentioning

confidence: 99%

Leaf gene expression trajectories during the growing season are consistent between sites and years in American beech

Sezen

Shue

Worthy

et al. 2022

Preprint

View full text Add to dashboard Cite

Transcriptomics, the quantification of gene expression, provides a versatile tool for ecological monitoring. Here, we show that through genome-guided profiling of transcripts mapping to 33,042 loci, gene expression differences can be discerned among multi-year and seasonal leaf samples collected from American beech trees at two latitudinally separated sites. Despite a bottleneck imposed due to large-scale post-Columbian deforestation, the SNP-based population genetic background analysis has yielded sufficient variation to account for differences between populations and among individuals. Our time series of expression analyses during spring-summer and summer-fall transitions for two consecutive years involved 4197 differentially expressed protein coding genes. A global comparison of 12 seasons has revealed that spring gene expression sets the pace for the rest of the growing season. Using \textit{Populus} orthologs of the differentially expressed genes, we reconstructed a protein-protein interactome as a representation of the leaf physiological states of trees during the seasonal transitions. Gene set enrichment analysis revealed GO terms that highlight molecular functions and biological processes possibly influenced by abiotic forcings such as recovery from drought and response to excess precipitation. Further, based on 324 co-regulated transcripts, we focused on a subset of terms that could be putatively attributed to phenological shifts due to late spring. Our conservative results indicate that extended transcriptome-based monitoring of forests can capture ranges of responses arising from other factors including air quality, chronic disease as well as herbivore outbreaks that require activation and/or downregulation of genes collectively tuning reaction norms needed for the survival of long living trees such as the American beech.

show abstract

How the stomate got his pore: very long chain fatty acids and a structural cell wall protein sculpt the guard cell outer cuticular ledge

Abstract: This article is a Commentary on Tang et al. (2020), 228: 1880–1896.

Cited by 10 publications

References 18 publications

Leaf gene expression trajectories during the growing season are consistent between sites and years in American beech

Leaf gene expression trajectories during the growing season are consistent between sites and years in American beech

Synthesis and import of GDP‐l‐fucose into the Golgi affect plant–water relations

Leaf gene expression trajectories during the growing season are consistent between sites and years in American beech

Contact Info

Product

Resources

About