“…Traditionally, beef quality has been influenced and evaluated by three general criteria: tenderness, juiciness, and flavor ( Boleman et al, 1997 ), of which, juiciness and flavor are subjective and influenced by several factors, such as cooking style, consumer preferences, and ethnic, cultural habits. However, tenderness is one of the most important factors influencing the quality and can be quantitatively and objectively measured by the Warner–Bratzler shear forces (WBSF) ( Huffman et al, 1996 ; Ferraris et al, 2013 ; Hocquette et al, 2013 ; Morales et al, 2013 ; Nogalski et al, 2018 ). Tenderness is a complex trait influenced by different structural components, such as the myofibrillar mass, sarcoplasmic proteins, intramuscular fat, and connective tissue, etc .…”