How Tim proteins differentially exploit membrane features to attain robust target sensitivity

Kerr, Daniel H.; Gong, Zhiliang; Suwatthee, Tiffany; Luoma, Adrienne; Roy, Sudip; Scarpaci, Renee; Hwang, Hyeondo Luke; Henderson, J. Michael; Cao, Kathleen D.; Bu, Wei; Lin, Binhua; Tietjen, Gregory T.; Steck, Theodore L.; Adams, Erin J.; Lee, Ka Yee C.

doi:10.1016/j.bpj.2021.09.016

Cited by 10 publications

(2 citation statements)

References 82 publications

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“…Additionally, the composition of the plasma membrane can prevent the insertion of receptors into the bilayer and induce changes in their topological orientation (34–36). TIM proteins have been shown to preferentially enter the lipid bilayer among unsaturated phospholipids rather than saturated ones (35). Consequently, we hypothesize that ΔCDC50 cells might undergo a redistribution of membrane proteins and a decrease in the proper insertion of these membrane receptors ( i.e., TIM and TYRO3), resulting in increased CHIKV release.…”

Section: Discussionmentioning

confidence: 99%

“…In general, transmembrane proteins (e.g., TIM, AXL, TYRO3) can disrupt fluidity within the plasma membrane which can trigger changes in the translocation of specific lipids (34). Additionally, the composition of the plasma membrane can prevent the insertion of receptors into the bilayer and induce changes in their topological orientation (34)(35)(36).…”

Section: Discussionmentioning

confidence: 99%

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Chikungunya Virus Release is Reduced by TIM-1 Receptors Through Binding of Envelope Phosphatidylserine

Reyes Ballista,

Hoover,

Noble

et al. 2024

Preprint

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T-cell immunoglobin and mucin domain protein-1 (TIM-1) mediates entry of Chikungunya virus (CHIKV) into some mammalian cells through the interaction with envelope phospholipids. While this interaction enhances entry, TIM has been shown to tether newly formed HIV and Ebola virus particles, limiting their efficient release. In this study, we investigate the ability of surface receptors such as TIM-1 to sequester newly budded virions on the surface of infected cells. We established a luminescence reporter system to produce Chikungunya viral particles that integrate nano-luciferase and easily quantify viral particles. We found that TIM-1 on the surface of host cells significantly reduced CHIKV release efficiency in comparison to other entry factors. Removal of cell surface TIM-1 through direct cellular knock-out or altering the cellular lipid distribution enhanced CHIKV release. Over the course of infection, CHIKV was able to counteract the tethering effect by gradually decreasing the surface levels of TIM-1 in a process that appears to be mediated by the nonstructural protein 2. This study highlights the importance of phosphatidylserine receptors in mediating not only the entry of CHIKV but also its release and could aid in developing cell lines capable of enhanced vaccine production.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Chikungunya Virus Release is Reduced by TIM-1 Receptors Through Binding of Envelope Phosphatidylserine

Reyes Ballista,

Hoover,

Noble

et al. 2024

Preprint

View full text Add to dashboard Cite

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Extrusion: A New Method for Rapid Formulation of High‐Yield, Monodisperse Nanobubbles

Counil

Abenojar

Perera

et al. 2022

Small

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Shell‐stabilized gas microbubbles (MB) and nanobubbles (NB) are frequently used for biomedical ultrasound imaging and therapeutic applications. While it is widely recognized that monodisperse bubbles can be more effective in these applications, the efficient formulation of uniform bubbles at high concentrations is difficult to achieve. Here, it is demonstrated that a standard mini‐extruder setup, commonly used to make vesicles or liposomes, can be used to quickly and efficiently generate monodisperse NBs with high yield. In this highly reproducible technique, the NBs obtained have an average diameter of 0.16 ± 0.05 µm and concentration of 6.2 ± 1.8 × 1010 NBs mL−1 compared to 0.32 ± 0.1 µm and 3.2 ± 0.7 × 1011 mL−1 for NBs made using mechanical agitation. Parameters affecting the extrusion and NB generation process including the temperature, concentration of the lipid solution, and the number of passages through the extruder are also examined. Moreover, it is demonstrated that extruded NBs show a strong acoustic response in vitro and a strong and persistent US signal enhancement under nonlinear contrast enhanced ultrasound imaging in mice. The extrusion process is a new, efficient, and scalable technique that can be used to easily produce high yield smaller monodispersed nanobubbles.

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Binding equations for the lipid composition dependence of peripheral membrane-binding proteins

Kerr,

Suwatthee,

Maltseva

et al. 2024

Biophysical Journal

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How Tim proteins differentially exploit membrane features to attain robust target sensitivity

Cited by 10 publications

References 82 publications

Chikungunya Virus Release is Reduced by TIM-1 Receptors Through Binding of Envelope Phosphatidylserine

Chikungunya Virus Release is Reduced by TIM-1 Receptors Through Binding of Envelope Phosphatidylserine

Extrusion: A New Method for Rapid Formulation of High‐Yield, Monodisperse Nanobubbles

Binding equations for the lipid composition dependence of peripheral membrane-binding proteins

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