Interventions to promote healthy and sustainable diets are urgently needed globally to curb growing obesity rates and greenhouse gas emissions. To date, little is known about consumers’ awareness of co-benefits of healthy and sustainable diets. Studies furthermore usually focus on Western countries, so lacking a global perspective. This study thus investigated cultural differences between Global South and Global North countries regarding perceptions of sustainable and healthy diets. The experiment used a 3 Meal Type (typical, healthy, sustainable) x 2 Region (Global North, Global South) mixed design. Participants (N = 74) were asked to self-serve three meals from a Fake Food Buffet (FFB) and to complete the Food Sustainability Knowledge Questionnaire (FSKQ). FSKQ scores did not differ between regions (W[71.82] = 0.58, p = .564). However, participants from the Global North self-served more vegetables, grains, and plant-based protein from the FFB compared to participants from the Global South (Fs[2, 72] ≥ 4.89 ps ≤ .003). Moreover, sustainable meals contained more vegetables, grains, legumes, and plant-based protein (Fs[2, 144] ≥ 4.26, ps ≤ .016), and less red meat, animal-based protein, and sugar than healthy or typical meals (Fs[2, 144] ≥ 11.77 ps ≤ .001). Many people are not aware of the similarities of healthy and sustainable diets. Furthermore, culture impact food choices and thus need to be considered when designing and implementing dietary interventions on a global scale.