How to Establish Acute Myeloid Leukemia Xenograft Models Using Immunodeficient Mice

Shan, Wulin; Ma, Xiaoling

doi:10.7314/apjcp.2013.14.12.7057

Cited by 9 publications

(4 citation statements)

References 95 publications

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“…Hematologic malignancies are a common disease in Asian Pacific areas (Feng et al, 2013;Jiang et al, 2013;Karami et al, 2013;Khodabandehloo et al, 2013;Kooshyar et al, 2013;Li et al, 2013;Liu et al, 2013;Ozbas-Gerceker et al, 2013;Qin et al, 2013;Shan et al, 2013). As our present study confirmed, the level of serum ferritin of patients with hematologic malignancies was significantly increased.…”

Section: Discussionsupporting

confidence: 86%

Elevated Serum Ferritin Levels in Patients with Hematologic Malignancies

Zhang

Su²,

Hu³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Section: Discussionsupporting

confidence: 86%

Elevated Serum Ferritin Levels in Patients with Hematologic Malignancies

Zhang

Su²,

Hu³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…All animal experiments were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animal and were approved by the Biomedical Ethics Committee of the Affiliated Hospital of Guiyang Medical College. Studies were conducted using 6-week-old NOD/SCID mice 19 (Beijing, China) were housed under specific pathogen-free conditions and allowed to acclimate for 2 weeks before experiments. The mice were housed (3 individuals per cage) and used at a weight of approximately 20.0 g. Human mononuclear cells (hMNCs) were isolated from bone marrow of B-ALL patients in a Ficoll-Hypaque gradient.…”

Section: Animals and Treatmentsmentioning

confidence: 99%

Overexpression of heme oxygenase‐1 in microenvironment mediates vincristine resistance of B‐cell acute lymphoblastic leukemia by promoting vascular endothelial growth factor secretion

Wang

Lü

et al. 2019

J of Cellular Biochemistry

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Chemoresistance often causes treatment failure of B‐cell acute lymphoblastic leukemia (B‐ALL). However, the mechanism remains unclear at present. Herein, overexpression of heme oxygenase‐1 (HO‐1) was found in the bone marrow stromal cells (BMSCs) from B‐ALL patients developing resistance to vincristine (VCR), a chemotherapeutic agent. Two B‐ALL cell lines Super B15 and CCRF‐SB were cocultured with BMSCs transfected with lentivirus to regulate the expression of HO‐1. Silencing HO‐1 expression in BMSCs increased the apoptotic rates of B‐ALL cell lines induced by VCR, whereas upregulating HO‐1 expression reduced the rate. Cell cycle can be arrested in the G2/M phase by VCR. In contrast, B‐ALL cells were arrested in the G0/G1 phase due to HO‐1 overexpression in BMSCs, which avoided damage from the G2/M phase. Vascular endothelial growth factor (VEGF) in BMSCs, as a key factor in the microenvironment‐associated chemoresistance, was also positively coexpressed with HO‐1. VEGF secretion was markedly increased in BMSCs with HO‐1 upregulation but decreased in BMSCs with HO‐1 silencing. B‐ALL cell lines became resistant to VCR when cultured with VEGF recombinant protein, so VEGF secretion induced by HO‐1 expression may promote the VCR resistance of B‐ALL cells. As to the molecular mechanism, the PI3K/AKT pathway mediated regulation of VEGF by HO‐1. In conclusion, this study clarifies a mechanism by which B‐ALL is induced to resist VCR through HO‐1 overexpression in BMSCs, and provides a novel strategy for overcoming VCR resistance in clinical practice.

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“…and intravenous (i.v.) routes [27][28][29]. Zhang et al, reported the successful engraftment of leukemic cells by i.p.…”

Section: In Vivo Studiesmentioning

confidence: 99%

Novel Polyethylene Glycol-Conjugated Triazole Derivative with High Thyrointegrin αvβ3 Affinity in Acute Myeloid Leukemia Management

Sudha

Godugu

Darwish

et al. 2021

Cancers

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(1) Background: Acute myeloid leukemia (AML) accounts for up to one-third of more than 60,000 leukemia cases diagnosed annually in the U.S. Primary AML cells express membrane αvβ3 integrin, which is associated with adverse prognosis and resistance to chemotherapies. A novel anticancer compound Polyethylene glycol-conjugated bi-TriAzole Tetraiodothyroacetic acid (P-bi-TAT) interacts with high affinity (Ki 0.3 nM) and specificity with the thyrointegrin αvβ3. We evaluated P-bi-TAT activities in two different AML models representing monocytic and myelocytic forms of acute leukemia. (2) Methods and Results: The in vivo AML models were established prior to initiation of treatment protocols by grafting human leukemia cells in immunocompromised mice. IVIS imaging scans revealed that leukemic colonies were extensively established throughout the bone marrow, liver, and lung of the untreated animals. In animals treated with P-bi-TAT at daily doses ranging from 1–10 mg/kg, subcutaneously for 2–3 weeks, IVIS imaging scans revealed 95% reduction in bone marrow colonies and leukemic colonies in liver and lung. Also, the leukemic cells were not detected in bone marrow samples of P-bi-TAT-treated animals. The anti-neoplastic effect of P-bi-TAT administration on leukemic cells was associated with marked inhibition of NF-κB activity. We conclude that experimental P-bi-TAT therapy in vivo appears extraordinarily effective against the two forms of human AML models in mice. Because the P-bi-TAT molecular target, thyrointegrin αvβ3, is consistently expressed in many, if not all, clinical AML samples, P-bi-TAT-based therapy seems to have significant clinical potential in treating most AML sub-types. Hence, P-bi-TAT represents a promising targeted therapeutic agent for AML patients.

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How to Establish Acute Myeloid Leukemia Xenograft Models Using Immunodeficient Mice

Cited by 9 publications

References 95 publications

Elevated Serum Ferritin Levels in Patients with Hematologic Malignancies

Elevated Serum Ferritin Levels in Patients with Hematologic Malignancies

Overexpression of heme oxygenase‐1 in microenvironment mediates vincristine resistance of B‐cell acute lymphoblastic leukemia by promoting vascular endothelial growth factor secretion

Novel Polyethylene Glycol-Conjugated Triazole Derivative with High Thyrointegrin αvβ3 Affinity in Acute Myeloid Leukemia Management

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