Currently, industrial sector has been facing challenges in terms issues where the industrial activities impact the environment. The industrial sector in Ngawi Regency is one of the sectors that contributes the fourth largest to the region's economy. This study identifys the factors that influence industrial sectors namely the aspects of revenue growth, sales growth, and production growth by using multiple linear regression analysis and applying Pentahelix Model as a collaboration model towards eco-industrial development. The findings of the study revealed the variables which significantly influenced the aspect of revenue growth namely raw material, capital, and infrastructure. The production growth is affected by raw materials, capital, marketing, production, and institutional. Supported by the result of industrial development factors, Pentahelix synergy is expected to be applied in the practice of ecoindustrial development based on the roles of each actor in Pentahelix.