How to Overcome Recalcitrance? Novel Strategies and Recent Advances in the Genetic Transformation of Grain Amaranth

Castellanos-Arévalo, Andrea Paola; Cabrera-Ponce, José Luis; Nava-Sandoval, Cecilia; Délano‐Frier, John Paul

doi:10.1007/978-3-030-72365-1_8

Cited by 2 publications

(1 citation statement)

References 110 publications

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“…HIGS requires that the crop species be amenable to genetic modification, a process that is time-consuming and costly and that remains unfeasible for many crop species. Despite this, new or modified transformation procedures for recalcitrant crop species are being steadily developed [177,178], and the next generation of transformation protocols seems imminent [179]. The delivery of genetic material for transformation using nanomaterials is one such innovation that can reduce transformation costs and improve reliability [180].…”

Section: Potential Limitations Of the Higs Approachmentioning

confidence: 99%

RNA-Based Control of Fungal Pathogens in Plants

Mann

Sawyer

Gardiner

et al. 2023

IJMS

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Our duty to conserve global natural ecosystems is increasingly in conflict with our need to feed an expanding population. The use of conventional pesticides not only damages the environment and vulnerable biodiversity but can also still fail to prevent crop losses of 20–40% due to pests and pathogens. There is a growing call for more ecologically sustainable pathogen control measures. RNA-based biopesticides offer an eco-friendly alternative to the use of conventional fungicides for crop protection. The genetic modification (GM) of crops remains controversial in many countries, though expression of transgenes inducing pathogen-specific RNA interference (RNAi) has been proven effective against many agronomically important fungal pathogens. The topical application of pathogen-specific RNAi-inducing sprays is a more responsive, GM-free approach to conventional RNAi transgene-based crop protection. The specific targeting of essential pathogen genes, the development of RNAi-nanoparticle carrier spray formulations, and the possible structural modifications to the RNA molecules themselves are crucial to the success of this novel technology. Here, we outline the current understanding of gene silencing pathways in plants and fungi and summarize the pioneering and recent work exploring RNA-based biopesticides for crop protection against fungal pathogens, with a focus on spray-induced gene silencing (SIGS). Further, we discuss factors that could affect the success of RNA-based control strategies, including RNA uptake, stability, amplification, and movement within and between the plant host and pathogen, as well as the cost and design of RNA pesticides.

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Section: Potential Limitations Of the Higs Approachmentioning

confidence: 99%

RNA-Based Control of Fungal Pathogens in Plants

Mann

Sawyer

Gardiner

et al. 2023

IJMS

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Comparative Analysis Reveals Changes in Some Seed Properties in Amaranth Mutant Variety ‘Zobor’ (A. hypochondriacus × A. hybridus)

et al. 2021

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The aim of our long-term research program is to improve the quality and quantity of amaranth production through mutation breeding using γ-radiation. In this paper, we present the characterization of the new variety ‘Zobor’ of A. hypochondriacus × A. hybridus developed by radiation-induced mutagenesis of hybrid K-433. Multiyear phenotypic characterization of an important yield parameter (1000-seed weight) showed that the studied mutant variety ‘Zobor’ has an advantage in seed weight over the nonirradiated control seeds of K-433 with predictable performance of this yield trait. ‘Zobor’ exhibited changes in seed morphometric parameters, starch particle size, and pasting properties with no change in amylose content and swelling power. Moreover, the seeds of ‘Zobor’ showed the significantly highest folate content among selected amaranth varieties. The mutant variety could, therefore, be interesting for the development of functional foods and as a low-management crop, attractive for cultivation in Europe.

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How to Overcome Recalcitrance? Novel Strategies and Recent Advances in the Genetic Transformation of Grain Amaranth

Cited by 2 publications

References 110 publications

RNA-Based Control of Fungal Pathogens in Plants

RNA-Based Control of Fungal Pathogens in Plants

Comparative Analysis Reveals Changes in Some Seed Properties in Amaranth Mutant Variety ‘Zobor’ (A. hypochondriacus × A. hybridus)

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