Plants, autotrophic organisms, in addition to the primary metabolism present in all living beings, have a secondary metabolism that allows them to produce and accumulate compounds of diverse chemical nature. These secondary metabolism compounds are termed secondary metabolites, differentially distributed among taxonomic groups, exhibit biological properties, many perform ecological functions and are characterized by their different uses and applications as medicines, insecticides, herbicides, perfumes or dyes, among others. Research into natural products has become a strong field of research related to the search for new molecules with diverse biological activities. From this, the potential sources for the search of these components have diversified enormously compromising the research of plants to the study of marine organisms and endophyte sources. This paper intends to divulge results of academic aspects of a research internship in Brazil with a duration of two months, through which a neuroprotective activity test was implemented in the Biotechnology and Bioassay Laboratory of the Chemistry Institute of the Federal University of Minas Gerais-Brazil; and also to expose of in vitro methodology others activities and developed routinely in the laboratory, to implement them in the Biotechnology-Natural Products Group of the Technological University of Pereira, related to the maintenance and research in endophytic fungi.