How to store autologous bone graft perioperatively: an in vitro study

Maus, Uwe; Andereya, S.; Gravius, Sascha; Siebert, Christian H.; Schippmann, Thomas; Ohnsorge, J. A. K.; Niedhart, Christopher

doi:10.1007/s00402-008-0616-8

Cited by 27 publications

(20 citation statements)

References 25 publications

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“…Maus et al [32] wiesen nach, dass trockene Aufbewahrung von entnommener Spongiosa zu einer signifikanten Einschränkung des Zellstoffwechsels führt.…”

Section: Die Einheilung Beeinflussende Faktorenunclassified

Rekonstruktion segmentaler Knochendefekte

Tjardes

Otchwemah

Hausmann

et al. 2012

Trauma Berufskrankh

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“…Maus et al [32] wiesen nach, dass trockene Aufbewahrung von entnommener Spongiosa zu einer signifikanten Einschränkung des Zellstoffwechsels führt.…”

Section: Die Einheilung Beeinflussende Faktorenunclassified

Rekonstruktion segmentaler Knochendefekte

Tjardes

Otchwemah

Hausmann

et al. 2012

Trauma Berufskrankh

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“…Saline solution, easily obtained, can also be used for bone graft storage with favorable in vitro results. 2 Currently, no studies have attempted to evaluate the in vivo effect of PPP as an alternative storage media, although there is evidence of the positive effect of PPP when compared with saline solution in an in vitro model. 6 Therefore, the aim of this study was to evaluate in vivo the effect of storage autogenous bone grafts in dry, saline solution, and PPP media during the transsurgical time.…”

Section: Introductionmentioning

confidence: 98%

“…7,8 Experimental evidence demonstrates that the living cells within a fresh bone graft contribute to osteogenesis within and around the graft. 2 If the graft cells are not viable or lost before implantation, little or no bone is produced, whereas in an intact living graft, abundant new bone appears. [7][8][9] Even with the occurrence of deleterious effects due to inappropriate graft handling during the transsurgical period, osteogenic cells may survive and contribute to the synthesis of new bone after transplantation under favorable conditions.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3] Autogenous bone grafts are more predictable when compared with other biomaterials because of their biological properties, 4 and they are considered the gold standard for bone reconstruction. 1,2,4 Graft volume maintenance and early bone remodeling are important factors in the success of these surgical procedures. 5 During a bone-grafting procedure, there is usually a delay between removal of the graft and its stabilization.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Different Storage Media on the Regenerative Potential of Autogenous Bone Grafts: A Histomorphometrical Analysis in Rabbits

Rocha

Batista

Zanetta‐Barbosa

et al. 2013

Journal of Oral Implantology

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The success of autogenous bone graft is related to the graft cell viability. In bone-grafting procedures, harvested grafts are often maintained in extraoral media while the recipient site is prepared. The aim of this study was to evaluate in vivo the effect of storage media over autogenous bone grafts during the transsurgical time. Two grafts were removed bilaterally from the calvaria of 18 rabbits. One graft was immediately fixed in the mandibular angle (control group), and the other was maintained in air exposure (dry group), 0.9% NaCl solution (saline group), or platelet-poor plasma (PPP group) during 30 minutes and stabilized in the symmetrical location of control grafts. After 28 days, the animals were euthanized and the bone fragments were removed, demineralized, and embedded in paraffin. Histological evaluation was performed under light microscope. Empty lacunae and bone graft area quantification were carried out for the sections. The histomorphometrical analysis revealed reduction of the graft area and increase of empty lacunae in the dry group when compared with control. No significant differences were found in the number of empty lacunae or bone graft area between the saline group and its control and also between the PPP group and its control. The dry group showed more empty lacunae and less graft area than the saline and PPP groups. In accordance with the results, PPP and physiologic solution demonstrated osteocyte preservation and bone graft area maintenance, being satisfactory storage media for autogenous bone grafts during the transsurgical period.

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“…Fusion can be achieved with autologous, allogenic or xenogenic bone grafts which are inserted between the vertebral bodies. Other materials and systems, such as titanium cages, are becoming more and more important, whereas spondylodesis with autologous tricortical bone graft represents the current clinical standard in many hospitals [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Risk of graft fracture after dorso-ventral thoraco-lumbar spondylodesis: is there a correlation with graft size?

et al. 2011

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Study design Retrospective clinical study in patients with dorso-ventral thoraco-lumbar spondylodesis. Objective To investigate whether the ratio between graft cross sectional area and the surface area ofthe adjacent endplates has any effect on the midterm stability of the spondylodesis. Summary of background data Dorso-ventral spondylodesis in the region of the thoraco-lumbar spine is one of the most frequent operations in orthopaedic surgery. Anterior stabilization with autologous iliac crest graft currently is a standard approach in many hospitals. Although numerous recommendations are given how to perform this technique, no clinical advice is available with regard to minimum graft size. Methods Sixty-four-slice CT-scans were obtained from 82 patients 4-12 months after posterior spondylodesis with anterior implantation of iliac crest graft and stabilization with an internal fixator. The scans were analyzed using image analysis software. First, the cross sectional area of the graft was calculated and then the surface area of the adjacent endplates. The ratio between graft cross sectional area and endplate surface area was then calculated from these two values. The grafts were then evaluated in sagittal reconstruction for signs of fracture. Results The probability for graft fracture in autologous tricortical grafts was [0.1% (p \ 0.001) if the graft cross sectional area exceeded 23.9% of the surface area of the adjacent endplates. Patients with lower ratio values had a higher fracture risk and below a value of 10% all grafts fractured. Conclusion The relationship between graft cross sectional area and adjacent endplate area has an important effect on graft midterm stability in ventral spondylodesis of the thoraco-lumbar spine. In our opinion, the risk of graft fractures in dorso-ventral spondylodesis can be reduced by implantation of an appropriately sized graft without any additional procedures or instrumentation.

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How to store autologous bone graft perioperatively: an in vitro study

Abstract: Perioperative storage of cancellous bone under dry conditions should be avoided. The bone graft should be stored in saline or 5% glucose solution or a moistened swab.

Cited by 27 publications

References 25 publications

Rekonstruktion segmentaler Knochendefekte

Rekonstruktion segmentaler Knochendefekte

Effect of Different Storage Media on the Regenerative Potential of Autogenous Bone Grafts: A Histomorphometrical Analysis in Rabbits

Risk of graft fracture after dorso-ventral thoraco-lumbar spondylodesis: is there a correlation with graft size?

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