How to study biofilms: technological advancements in clinical biofilm research

Cleaver, Leanne; Garnett, James A.

doi:10.3389/fcimb.2023.1335389

Cited by 2 publications

References 190 publications

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Synthetic microbial ecology: engineering habitats for modular consortia

Chen,

Destouches,

Cook

et al. 2024

Journal of Applied Microbiology

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Microbiomes, the complex networks of micro-organisms and the molecules through which they interact, play a crucial role in health and ecology. Over at least the past two decades, engineering biology has made significant progress, impacting the bio-based industry, health, and environmental sectors; but has only recently begun to explore the engineering of microbial ecosystems. The creation of synthetic microbial communities presents opportunities to help us understand the dynamics of wild ecosystems, learn how to manipulate and interact with existing microbiomes for therapeutic and other purposes, and to create entirely new microbial communities capable of undertaking tasks for industrial biology. Here, we describe how synthetic ecosystems can be constructed and controlled, focusing on how the available methods and interaction mechanisms facilitate the regulation of community composition and output. While experimental decisions are dictated by intended applications, the vast number of tools available suggests great opportunity for researchers to develop a diverse array of novel microbial ecosystems.

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Synthetic microbial ecology: engineering habitats for modular consortia

Chen,

Destouches,

Cook

et al. 2024

Journal of Applied Microbiology

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The landscape of the bacteriome and mycobiome at different stages of root caries and the cross-kingdom interactions of the core species

Ji,

Xiong,

et al. 2024

Preprint

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Background The unbalanced oral microbiome is considered the key pathogenic agent for root caries, the most common tooth disease in elderly individuals; however, the bacteriome and mycobiome at different stages of root caries, especially from the same individual, are not clear. Results In this study, superficial and deep root caries plaques from thirty patients with different levels of root caries simultaneously in the oral cavity were collected, whereas sound root surface plaques from their healthy teeth served as caries-free controls. The full-length sequencing results of 16S and 18S rDNA analysis via the self-controlled method on the basis of the same patients indicated that the different stages of root caries represented different microbiota, including Streptococcus mutans and Actinomyces sp. HMT448 in superficial root caries and Prevotella sp. in deep root caries. Candida albicans was the most abundant fungal species from all the plaques, while it showed strong interspecies interactions with S. mutans and Actinomyces sp. Their interactions were closely associated with the different stages of root caries. An in vitro model further confirmed that C. albicans was able to increase the growth, biofilm formation and cariogenicity of S. mutans and A. viscosus through the activation of the arginine biosynthesis pathway, indicating its key roles in the development of root caries. Conclusion Our study revealed the first landscape of the microbiome from different stages of root caries and indicated that targeting the interactions of core species may be a practical way to prevent and treat clinical root caries.

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How to study biofilms: technological advancements in clinical biofilm research

Cited by 2 publications

References 190 publications

Synthetic microbial ecology: engineering habitats for modular consortia

Synthetic microbial ecology: engineering habitats for modular consortia

The landscape of the bacteriome and mycobiome at different stages of root caries and the cross-kingdom interactions of the core species

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