How will warming affect the growth and body size of the largest extant amphibian? More than the temperature–size rule

Zhu, Wei; Zhao, Tian; Zhao, Chunlin; Li, Cheng; Xie, Feng; Liu, Jiongyu; Jiang, Jianping

doi:10.1016/j.scitotenv.2022.160105

Cited by 5 publications

(2 citation statements)

References 55 publications

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“…Indeed, although our study focuses on a single breeding population, microhabitat use can vary among morphotypes and even among different developmental stages, allowing for trophic niche expansion and variation resulting in lower intraspecific competition (Denoël & Andreone, 2003; Lejeune et al., 2021). At the same time, metabolic rates can be strongly influenced by the long‐term shifts of temperature and humidity due to climate change, affecting the energy allocated to reproduction, feeding and survival, especially on ectotherms (Norin & Metcalfe, 2019; Riemer et al., 2018; Wu et al., 2022; Zhu et al., 2023). Changes in the genetic composition of populations can lead to shifts in average body size over time, thus understanding the evolutionary potential of the trait, especially in wild amphibian populations that are sensitive to environmental alterations, can increase our ability to identify and prioritize conservation efforts towards more resilient populations.…”

Section: Discussionmentioning

confidence: 99%

Genetic insight into a polygenic trait using a novel genome‐wide association approach in a wild amphibian population

Toli,

Kemppainen,

Bounas

et al. 2024

Molecular Ecology

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Body size variation is central in the evolution of life‐history traits in amphibians, but the underlying genetic architecture of this complex trait is still largely unknown. Herein, we studied the genetic basis of body size and fecundity of the alternative morphotypes in a wild population of the Greek smooth newt (Lissotriton graecus). By combining a genome‐wide association approach with linkage disequilibrium network analysis, we were able to identify clusters of highly correlated loci thus maximizing sequence data for downstream analysis. The putatively associated variants explained 12.8% to 44.5% of the total phenotypic variation in body size and were mapped to genes with functional roles in the regulation of gene expression and cell cycle processes. Our study is the first to provide insights into the genetic basis of complex traits in newts and provides a useful tool to identify loci potentially involved in fitness‐related traits in small data sets from natural populations in non‐model species.

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Section: Discussionmentioning

confidence: 99%

Genetic insight into a polygenic trait using a novel genome‐wide association approach in a wild amphibian population

Toli,

Kemppainen,

Bounas

et al. 2024

Molecular Ecology

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“…To date, dermal swabs have been used in amphibians for DNA collection (Prunier et al, 2012) and disease profiling for two chytridiomycosis species: Batrachochytrium dendrobatidis (Bd) and Batrachochytrium salamandrivorans (Bsal), as well as frogvirus 3-like ranavirus (FV3; Standish et al, 2018). Mucosal sampling has been validated as a viable technique for metabolomic profiling in fish (Ekman et al, 2015), as well as peptide (reviewed in Barros et al, 2022), amino acid (Dejing, 2010;Rickrode et al, 1986), and transcriptomic analyses in amphibians (Zhu et al, 2022(Zhu et al, , 2023. However, a comparison of the metabolomic profile from full-body dermal swabs relative to hepatic tissue samples among amphibians following exposure to a chemical stressor has not yet been published.…”

Section: Introductionmentioning

confidence: 99%

Validation of Salamander Dermal Mucus Swabs as a Novel, Nonlethal Approach for Amphibian Metabolomics and Glutathione Analysis Following Pesticide Exposure

Van Meter,

Glinski,

Wanat

et al. 2024

Enviro Toxic and Chemistry

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Evaluating biomarkers of stress in amphibians is critical to conservation, yet current techniques are often destructive and/or time‐consuming, which limits ease of use. In the present study, we validate the use of dermal swabs in spotted salamanders (Ambystoma maculatum) for biochemical profiling, as well as glutathione (GSH) stress response following pesticide exposure. Thirty‐three purchased spotted salamanders were acclimated to laboratory conditions at Washington College (Chestertown, MD, USA) for 4 weeks. Following acclimation, salamanders were randomly sorted into three groups for an 8‐h pesticide exposure on soil: control with no pesticide, 2,4‐dichlorophenoxyacetic acid (2,4‐D), or chlorpyrifos. Before and after exposure, mucus samples were obtained by gently rubbing a polyester‐tipped swab 50 times across the ventral and dorsal surfaces. Salamanders were humanely euthanized and dissected to remove the brain for acetylcholinesterase and liver for GSH and hepatic metabolome analyses, and a whole‐body tissue homogenate was used for pesticide quantification. Levels of GSH were present in lower quantities on dermal swabs relative to liver tissues for chlorpyrifos, 2,4‐D, and control treatments. However, 2,4‐D exposures demonstrated a large effect size increase for GSH levels in livers (Cohen's d = 0.925, p = 0.036). Other GSH increases were statistically insignificant, and effect sizes were characterized as small for 2,4‐D mucosal swabs (d = 0.36), medium for chlorpyrifos mucosal swabs (d = 0.713), and negligible for chlorpyrifos liver levels (d = 0.012). The metabolomics analyses indicated that the urea cycle, alanine, and glutamate metabolism biological pathways were perturbed by both sets of pesticide exposures. Obtaining mucus samples through dermal swabbing in amphibians is a viable technique for evaluating health in these imperiled taxa. Environ Toxicol Chem 2024;00:1–12. © 2024 SETAC

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Antioxidant synergistic anti‐inflammatory effect in the MAPK/NF‐κB pathway of peptide KGEYNK (KK‐6) from giant salamander (Andrias davidianus)

Zhan,

Wu,

et al. 2024

J Sci Food Agric

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BACKGROUNDGiant salamander protein peptide is a peptide with rich functional properties. Giant salamander protein peptide KGEYNK (KK‐6) is a peptide with both antioxidant and anti‐inflammatory properties. The antioxidant and anti‐inflammatory mechanisms of KK‐6 are still unclear. When we studied the functional mechanism of KK‐6, we found that the antioxidant property of KK‐6 has a synergistic and promoting effect on anti‐inflammatory properties.RESULTSKK‐6 enhances cellular resistance to LPS via the MAPK/NF‐κB signaling pathway, leading to increased levels of inflammatory factors: interleukin‐1β (764.81 ng mL−1), interleukin‐6 (1.06 ng mL−1) and tumor necrosis factor‐α (4440.45 ng mL−1). KK‐6 demonstrates potent antioxidant properties by activating the Nrf2 signaling pathway, resulting in elevated levels of antioxidant enzymes (glutathione peroxidase: 0.03 μg mL−1; superoxide dismutase: 0.589 μg mL−1) and a reduction in the concentration of the oxidative product malondialdehyde (967.05 μg mL−1).CONCLUSIONOur findings highlight the great potential of KK‐6, a peptide extracted from giant salamander protein, as a remedy for intestinal inflammation. Through its dual role as an antioxidant and anti‐inflammatory agent, KK‐6 offers a promising avenue for alleviating inflammation‐related damage and oxidative stress. This study lays the foundation for further exploration of giant salamander products and highlights their importance in health and novel food development. © 2024 Society of Chemical Industry.

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How will warming affect the growth and body size of the largest extant amphibian? More than the temperature–size rule

Cited by 5 publications

References 55 publications

Genetic insight into a polygenic trait using a novel genome‐wide association approach in a wild amphibian population

Genetic insight into a polygenic trait using a novel genome‐wide association approach in a wild amphibian population

Validation of Salamander Dermal Mucus Swabs as a Novel, Nonlethal Approach for Amphibian Metabolomics and Glutathione Analysis Following Pesticide Exposure

Antioxidant synergistic anti‐inflammatory effect in the MAPK/NF‐κB pathway of peptide KGEYNK (KK‐6) from giant salamander (Andrias davidianus)

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