HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas

Jäger, Daniela; Barth, Thomas F.E.; Brüderlein, Silke; Scheuerle, Angelika; Rinner, Beate; Witzleben, Adrian von; Lechel, André; Meyer, Patrick; Mayer-Steinacker, Regine; Baer, Alexandra von; Schultheiß, Markus; Wirtz, Christian Rainer; Möller, Peter; Mellert, Kevin

doi:10.1038/s41598-017-02174-5

Cited by 27 publications

(17 citation statements)

References 54 publications

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“…Feng et al [ 6 ] suggest that targeting PD-L1 may be a novel immunotherapeutic strategy for chordoma clinical trials. The findings of Hu et al [ 20 , 21 ] highlight that fibroblast growth factor receptor (FGFR)/ methyl ethyl ketone (MEK)/ extracellular signal regulated kinase (ERK)/brachyury pathway coordinately regulates chordoma cell growth and survival and may represent a novel chemotherapeutic target for chordoma. Jäger et al [ 22 ] suggest that the differential expression of HOX genes in chordomas of the clivus and sacrum suggests an oncofetal mechanism in gene regulation linked to the anatomic site.…”

Section: Discussionmentioning

confidence: 99%

A giant lumbar chordoma

Zhou

Hu²,

Wu³

et al. 2018

Medicine

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Rationale:Chordomas are malignant neoplasms derived from incomplete regression of notochordal tissue along the craniococcygeal axis.It is rare for Chordoma arising from the lumbar spine and the traditional long-term prognosis is typically poor.Patient concerns:The persistent pain in the left side of the waist about 2 years.Diagnoses:Chordoma.Interventions:The patient was treated with surgical resection of the total tumor, followed by the spinal internal fixation of L1 to L2 with pedicle screws.Outcomes:After 5 month follow-up,we find the recurrence in the original lesion.At the 15 month follow-up,the patient was dead after a lot of times revisit by various doctor.Lessons:So It is suggest that the diagnosis should be carried out accurately at the early stage, the lesions and source of lesions should be cut away as broadly as possible, also the radiation and chemotherapy should be carried out after the operation as necessary.

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Section: Discussionmentioning

confidence: 99%

A giant lumbar chordoma

Zhou

Hu²,

Wu³

et al. 2018

Medicine

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“…22 Other studies have shown that HOXA7, HOXA9 and HOXA10 play important roles in the development of the anterior and posterior body axes. 23 Due to its pro-proliferation and pro-metastasis effects, HOXA7 is highly expressed in many diseases, including liver cancer, hepatocellular carcinoma and breast cancer. [15][16][17] In view of the characteristics of HOXA7, we also investigated whether it affected the proliferation and metastasis of trophoblast cells.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA LINC00511 regulates the proliferation, apoptosis, invasion and autophagy of trophoblast cells to mediate pre‐eclampsia progression through modulating the miR‐31‐5p/homeobox protein A7 axis

Dong

Zhang

Chen

et al. 2020

J of Obstet and Gynaecol

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Aim Pre‐eclampsia (PE) is the usual complication during pregnancy. Long noncoding RNAs are essential regulatory factors in many diseases. Nevertheless, the role of LINC00511 in the development of PE has not been fully elucidated. Methods The expression of LINC00511, homeobox protein A7 (HOXA7) and miR‐31‐5p was determined by quantitative real‐time polymerase chain reaction. The levels of HOXA7 protein and autophagy‐related proteins were measured by western blot analysis. Besides, cell proliferation was evaluated using cell counting kit 8 and colony formation assays. The apoptosis and invasion of cells were detected via flow cytometry and transwell assay, respectively. Further, the interaction between miR‐31‐5p and LINC00511 or HOXA7 was confirmed by dual‐luciferase reporter assay. Results The LINC00511 and HOXA7 expression levels were decreased in placental tissues of PE patients, and the expression levels of both were positively correlated. LINC00511 knockdown suppressed proliferation, invasion and autophagy, while enhanced apoptosis in trophoblast cells. Meanwhile, the elevated HOXA7 expression promoted proliferation, invasion, autophagy, and inhibited the apoptosis of trophoblast cells. Besides, overexpression of HOXA7 also could reverse the effect of LINC00511 knockdown on the biological function of trophoblast cells. Further experiments confirmed that miR‐31‐5p could be sponged by LINC00511 and could target HOXA7. Also, miR‐31‐5p mimic could invert the promoting effect of LINC00511 overexpression on the biological function of trophoblast cells. Conclusion LINC00511 expression was crucial for maintaining the normal function of trophoblast cells, and the decreased its expression might promote the progress of PE, which might provide some theoretical strategies for reducing the development of PE.

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“…#: 51304). STR analysis was performed comparing the parental tumors with the corresponding cell lines and genotyping them and the control cell lines by using a standard protocol 41 .…”

Section: Methodsmentioning

confidence: 99%

“…Protein isolation and Western blots were performed using standard protocols as described elsewhere 41 . The following primary antibodies were used: R2 (Santa Cruz Biotechnology, Dallas, USA, cat.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Three Novel H3F3A-mutated Giant Cell Tumor Cell Lines and Targeting of Their Wee1 Pathway

Lübbehüsen

Lüke

Seeling

et al. 2019

Sci Rep

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The giant cell tumor of bone (GCTB) is a locally aggressive primary bone tumor that is composed of mononuclear stroma cells, scattered macrophages, and multinucleated osteoclast-like giant cells which cause pathologic osteolysis. The stroma cells represent the neoplastic population of the tumor and are characterized by the H3F3A mutation G34W. This point mutation is regarded as the driver mutation of GCTB. We have established three new stable H3F3A mutated GCTB cell lines: U-GCT1, U-GCT2, and U-GCT3M. MK-1775 is a Wee1-kinase inhibitor which has been used for blocking of sarcoma growth. In the cell lines we detected Wee1, Cdk1, Cyclin B1, H3K36me3, and Rrm2 as members of the Wee1 pathway. We analyzed the effect of MK-1775 and gemcitabine, alone and in combination, on the growth of the cell lines. The cell lines showed a significant reduction in cell proliferation when treated with MK-1775 or gemcitabine. The combination of both agents led to a further significant reduction in cell proliferation compared to the single agents. Immunohistochemical analysis of 13 GCTB samples revealed that Wee1 and downstream-relevant members are present in GCTB tissue samples. Overall, our work offers valuable new tools for GCTB studies and presents a description of novel biomarkers and molecular targeting strategies.

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HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas

Cited by 27 publications

References 54 publications

A giant lumbar chordoma

A giant lumbar chordoma

Long noncoding RNA LINC00511 regulates the proliferation, apoptosis, invasion and autophagy of trophoblast cells to mediate pre‐eclampsia progression through modulating the miR‐31‐5p/homeobox protein A7 axis

Characterization of Three Novel H3F3A-mutated Giant Cell Tumor Cell Lines and Targeting of Their Wee1 Pathway

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