HP-β-CD-PLGA nanoparticles improve the penetration and bioavailability of puerarin and enhance the therapeutic effects on brain ischemia–reperfusion injury in rats

Tao, Haiquan; Meng, Qing-Feng; Li, Minghui; Yu, Hui; Liu, Meifang; Du, Dan; Sun, Shichao; Yang, Haicheng; Wang, Yanming; Ye, Wei; Yang, Lizhuang; Zhu, Daling; Jiang, Chuanlu; Peng, Haisheng

doi:10.1007/s00210-012-0804-5

Cited by 41 publications

(15 citation statements)

References 23 publications

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“…In order to overcome this issue, we chemically modified PLGA using esterification reaction sequentially, as shown in Scheme 1. First, 1 3 nm AuNCs were conjugated to the PLGA polymer and subsequently these AuNCs were derivatized with hydroxypropyl β cyclodextrin (HP β CD), a cyclic drug host molecule 29, 30 that can be incorporated into polymeric NPs 31 . To that end the carboxyl groups of 11 mercaptoundecanoic acid (MUA) capped 3 nm AuNCs were reacted with hydroxyl groups of PLGA monomers using N,N′-dicyclohexylcarbodiimide (DCC) and 4 dimethylaminopyridine (DMAP), which resulted in the formation of an ester bond between the ligands of AuNCs and PLGA monomers.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy

Mieszawska

Kim

Gianella³

et al. 2013

Bioconjugate Chem.

108

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For advanced treatment of diseases such as cancer, multi component, multi functional nanoparticles hold great promise. In the current study we report the synthesis of a complex nanoparticle (NP) system with dual drug loading as well as diagnostic properties. To that aim we present a methodology where chemically modified poly(lactic co glycolic) acid (PLGA) polymer is formulated into a polymer lipid NP that contains a cytotoxic drug doxorubicin (DOX) in the polymeric core and an anti angiogenic drug sorafenib (SRF) in the lipidic corona. The NP core also contains gold nanocrystals (AuNCs) for imaging purposes and cyclodextrin molecules to maximize the DOX encapsulation in the NP core. In addition, a near infrared (NIR) Cy7 dye was incorporated in the coating. To fabricate the NP we used a microfluidics based technique that offers unique NP synthesis conditions, which allowed for encapsulation and fine tuning of optimal ratios of all the NP components. NP phantoms could be visualized with computed tomography (CT) and near infrared (NIR) fluorescence imaging. We observed timed release of the encapsulated drugs, with fast release of the corona drug SRF and delayed release of a core drug DOX. In tumor bearing mice intravenously administered NPs were found to accumulate at the tumor site by fluorescence imaging.

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Section: Resultsmentioning

confidence: 99%

Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy

Mieszawska

Kim

Gianella³

et al. 2013

Bioconjugate Chem.

108

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“…However, imperatorin and isoimperatorin showed better pharmacokinetic behavior compared with puerarin and daidzein. The bioavailability of puerarin is very low owing to its reverse physiochemical properties which block it from penetrating the blood–brain barrier (Tao et al ., ). The results of this research provides evidence that the effective ingredients of Baige capsule may play a significant role in the convalescent period of stroke.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous determination of four components in Baige capsule by HPLC: application to pharmacokinetics and tissue distribution of normal and middle cerebral artery occlusion rats

Wang

et al. 2013

Biomedical Chromatography

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A sensitive and specific high-performance liquid chromatography (HPLC) method was developed and applied to the pharmacokinetics for simultaneous identification and quantification of four components - puerarin, daidzein, imperatorin and isoimperatorin - in the plasma and tissues of normal and middle cerebral artery occlusion rats after oral administration of Baige capsule. Ferulic was used as the internal standard. The extraction procedure was composed of two independent steps. The plasma was prepared by liquid-liquid extraction with light petroleum-acetic ether (1:1, v/v) first and then protein was precipitated with methanol. The tissue samples were weighted and homogenated with normal saline, then the homogenate was prepared by liquid-liquid extraction and protein precipitation. The parameters of pharmacokinetics were calculated using DAS 2.1.1 software. The calibration curves of all four components in the plasma and tissue homogenates were in good linearity in the measured range with R(2) ≥ 0.9921. The relative standard deviation of the intra- and inter-day accuracy at different levels was less than ±18.0%. In conclusion, the established method was a simple and effective one to simultaneously detect all four components in the plasma and tissues of rats, and was successfully applied in the pharmacokinetics of Baige capsule.

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“…Also, the average size of infarction was significantly lower in 3 and 7 days after PUE-nanoparticle treatment than I/R control and free PUE groups; H&E (Haemotoxylin and Eosin) staining showed that the brain penetration of inflammatory cells and the neuronal pyknosis, karyolysis were significantly decreased in PUE-nanoparticle treated rats on days 3 and 7. In addition, I/R rats treated with PUE-nanoparticles significantly improved cortical EEG power, peak, area, frequency, and valley value on days 3 and 7 compared to I/R control and free PUE treated groups [ 108 ].…”

Section: Oral Administration Of Nanoparticles In Stroke Treatmentmentioning

confidence: 99%

Oral and Intra-nasal Administration of Nanoparticles in the Cerebral Ischemia Treatment in Animal Experiments: Considering its Advantages and Disadvantages

Alavian

Shams

2020

CCP

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Background: Over the past few decades, nanotechnology has dramatically advanced; from the precise strategies of synthesizing modern nanostructures to methods of entry into the body. Using nanotechnology in diagnosis, drug delivery, determining signaling pathways, and tissue engineering is great hope for the treatment of stroke. The drug-carrying nanoparticles are a way to increase drug absorption through the mouth or nose in treating the stroke. Objective: In this article, in addition to explaining pros and cons of oral and intra-nasal administration of nanoparticles in the brain ischemia treatment of animal models, the researchers introduce some articles in this field and briefly mentioned their work outcomes. Methods: A number of relevant published articles 183 were initially collected from three popular databases including PubMed, Google Scholar, and Scopus. The articles not closely related to the main purpose of the present work were removed from the study process. The present data set finally included 125 published articles. Results: Direct delivery of the drug to the animal brain through the mouth and nose has more therapeutic effects than systemic delivery of drugs. The strategy of adding drugs to the nanoparticles complex can potentially improve the direct delivery of drugs to the CNS. Conclusion: Despite the limitations of oral and intra-nasal routes, the therapeutic potential of oral and intra-nasal administration of nano-medicines is high in cerebral ischemia treatment.

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HP-β-CD-PLGA nanoparticles improve the penetration and bioavailability of puerarin and enhance the therapeutic effects on brain ischemia–reperfusion injury in rats

Cited by 41 publications

References 23 publications

Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy

Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy

Simultaneous determination of four components in Baige capsule by HPLC: application to pharmacokinetics and tissue distribution of normal and middle cerebral artery occlusion rats

Oral and Intra-nasal Administration of Nanoparticles in the Cerebral Ischemia Treatment in Animal Experiments: Considering its Advantages and Disadvantages

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