HPDS‐Hochdruckzelle zur Beobachtung mikroskopischer Phänomene unter Hochdruck

Abstract

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Microscopy under pressure—An optical chamber system for fluorescence microscopic analysis of living cells under high hydrostatic pressure

“…The thickness of the cover plate did not disturb the pressure broadening (Cheftel, 1992). The cell is described in detail elsewhere (Hartmann et al, 2003(Hartmann et al, , 2004.…”

“…A new cell for the in situ observation of microscopic objects during HHP treatment has recently been developed in our Institute (Technische Universitaet Muenchen; Freising-Weihenstephan, Germany), in cooperation with the Record Maschinenbau GmbH (Koenigsee, Germany). The HPDS (Hartmann, Pfeifer, Dornheim, Sommer) high-pressure cell withstands pressures up to 300 MPa and allows one to follow the effects of HHP treatment in situ, employing an inverse light microscope coupled to a CCD Camera (Hartmann et al, 2004;Hartmann et al, 2003). In life science research, fluorescence methods are a standard for the examination of biomaterials, e.g., the detection of apoptosis (Vermes et al, 1995).…”

Optical in situ analysis of starch granules under high pressure with a high pressure cell

“…The high pressure microscope is described in detail elsewhere (Hartmann et al, 2003) and consisted of an inverse light microscope (Leica DM IRB HC, Germany), a CCD-camera (Basler, Germany) and the HPDS high pressure cell connected to a screw press. A computer with an image analysis software (Aquinto, Germany) was coupled with the camera in order to evaluate and archive the pictures recorded.…”

“…Unstained and iodine stained starch suspensions were filled in the HPDS cell onto the microscope stage as described by Hartmann et al (2003) and during pressure build-up pictures were taken at 50 MPa intervals. At 300 MPa, the pressure was held for 1 h, while pictures were taken about every 10 min.…”

“…The Lehrstuhl für Maschinen-und Apparatekunde at the Technical University München (Freising-Weihenstephan, Germany) in cooperation with the mechanical engineering company Record Maschinenbau (Königsee, Germany) developed the so-called Hartmann, Pfeifer, Dornheim, Sommer-High Pressure Cell (HPDS High Pressure Cell) enabling microscopic in situ analyses under pressures of up to 300 MPa (Hartmann, Pfeifer, Dornheim, & Sommer, 2003). Aim of this work was to validate the efficiency of this newly developed HPDS High Pressure Cell and furthermore to gain insight into the process of pressure-induced starch gelatinisation.…”