hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway

Wan, Xinxing; Zhu, Liyong; Zhao, Liling; Peng, Lin; Xiong, Jing; Yang, Wenjun; Yuan, Jingjing; Fang, Liang; Zhang, Keke; Chen, Ke

doi:10.1038/s41419-021-03584-0

Cited by 9 publications

(10 citation statements)

References 55 publications

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“…The EthD-III staining experiments found that EthD-IIIpositive cells existed in the dentate gyrus and pyramidal cells in hippocampal and cortex tissues. The pyroptotic cells looked like cabbage or fried egg, with the nucleus located in the center [25]. The results showed that the level of pyroptosis was elevated in both the hippocampal and cortex tissues of septic hsf1 -/and hsf1 +/+ CLP models contrasted to the sham control, respectively.…”

Section: Pyroptosis Is Elevated In Brainmentioning

confidence: 88%

“…The induction of HSPs responsible for maturation, antioxidative protection, adiposis, etc., can be significantly affected by harmful stress such as reactive oxygen species (ROS) and inflammation [18][19][20]. Interacting with other signaling pathways, HSPs can produce a collective response against harmful stress like sepsis [21][22][23][24][25]. HSPs are evolutionarily conserved and involved in the cellular protective mechanism of heat shock response (HSR) to maintain protein homeostasis in almost all eukaryotic cells.…”

Section: Introductionmentioning

confidence: 99%

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HSF1 Alleviates Brain Injury by Inhibiting NLRP3-Induced Pyroptosis in a Sepsis Model

et al. 2023

Mediators of Inflammation

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Background. Sepsis, which could cause a systemic inflammatory response, is a life-threatening disease with a high morbidity and mortality rate. There is evidence that brain injury may be related to severe systemic infection induced by sepsis. The brain injury caused by sepsis could increase the risk of mortality in septic patients, which seriously affects the septic patient’s prognosis of survival. Although there remains a focus on sepsis research, clinical measures to prevent and treat brain injury in sepsis are not yet available, and the high mortality rate is still a big health burden. Therefore, it is necessary to investigate the new molecules or regulated pathways that can effectively inhibit the progress of sepsis. Objective. NLR family pyrin domain-containing 3 (NLRP3) increased in the procession of sepsis and functioned as the key regulator of pyroptosis. Heat shock factor 1 (HSF1) can protect organs from multiorgan dysfunction syndrome induced by lipopolysaccharides in mice, and NLRP3 could be inhibited by HSF1 in many organs. However, whether HSF1 regulated NLRP3 in sepsis-induced brain injury, as well as the detailed mechanism of HSF1 in brain injury, remains unknown in the sepsis model. In this research, we try to explore the relationship between HSF1 and NLRP3 in a sepsis model and try to reveal the mechanism of HSF1 inhibiting the process of brain injury. Methods. In this study, we used wild-type mice and hsf1-/- mice for in vivo research and PC12 cells for in vitro research. Real-time PCR and Western blot were used to analyze the expression of HSF1, NLRP3, cytokines, and pyrolytic proteins. EthD-III staining was chosen to detect the pyroptosis of the hippocampus and PC12 cells. Results. The results showed that HSF1 is negatively related to pyroptosis. The pyroptosis in cells of brain tissue was significantly increased in the hsf1-/- mouse model compared to hsf1+/+ mice. In PC12 cells, hsf1 siRNA can upregulate pyroptosis while HSF1-transfected plasmid could inhibit the pyroptosis. HSF1 could negatively regulate the NLRP3 pathway in PC12 cells, while hsf1 siRNA enhanced the pyroptosis in PC12 cells, which could be reversed by nlrp3 siRNA. Conclusion. These results imply that HSF1 could alleviate sepsis-induced brain injury by inhibiting pyroptosis through the NLRP3-dependent pathway in brain tissue and PC12 cells, suggesting HSF1 as a potential molecular target for treating brain injury in sepsis clinical studies.

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Section: Pyroptosis Is Elevated In Brainmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

HSF1 Alleviates Brain Injury by Inhibiting NLRP3-Induced Pyroptosis in a Sepsis Model

et al. 2023

Mediators of Inflammation

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“…Other genes may also be involved in different aspects of the anti-obesity regulatory process. For instance, the PPARG can regulate systemic insulin resistance, PPARG dysregulation will cause obesity, and HSP90AA1 can inhibit the reduction of adipogenesis through the in vivo pathway to trigger the anti-obesity effect [ 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Revealing Molecular Mechanisms of the Bioactive Saponins from Edible Root of Platycodon grandiflorum in Combating Obesity

Han,

Luo,

2024

Plants

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Obesity has emerged as a significant health concern, as it is a disease linked to metabolic disorders in the body and is characterized by the excessive accumulation of lipids. As a plant-derived food, Platycodon grandiflorum (PG) was reported by many studies, indicating that the saponins from PG can improve obesity effectively. However, the anti-obesity saponins from PG and its anti-obesity mechanisms have not been fully identified. This study identified the active saponins and their molecular targets for treating obesity. The TCMSP database was used to obtain information on 18 saponins in PG. The anti-obesity target of the PG saponins was 115 targets and 44 core targets. GO and KEGG analyses using 44 core anti-obesity genes and targets of PG-active saponins screened from GeneCards, OMIM, Drugbank, and DisGeNet showed that the PI3K-Akt pathway, the JAK-STAT pathway, and the MAPK pathway were the major pathways involved in the anti-obesity effects of PG saponins. BIOVIA Discovery Studio Visualizer and AutoDock Vina were used to perform molecular docking and process the molecular docking results. The molecular docking results showed that the active saponins of PG could bind to the major therapeutic obesity targets to play an obesity-inhibitory role. The results of this study laid the foundation for further research on the anti-obesity saponins in PG and their anti-obesity mechanism and provided a new direction for the development of functional plant-derived food. This research studied the molecular mechanism of PG saponins combating obesity through various signaling pathways, and prosapogenin D can be used to develop as a new potential anti-obesity drug.

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“…Also, circadian rhythm controls the majority of genes activity that involves various diseases including bone diseases, arthritis, aging, and metabolic diseases 32,37,38 . As one of the circadian controlled genes, NOTCH1 can be regulated by PER3 during adipogenesis 39 . H Angharad Watson found the L-selectin (CD62L) can increase the T cells to eliminate cancer cells 40 .…”

Section: Discussionmentioning

confidence: 99%

Identification of biomarkers and signaling pathways during the treatment of multiple myeloma

2021

Preprint

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Multiple myeloma (MM) is an incurable hematologic malignancy, which is characterized by increased bone marrow plasma cells, osteolytic lesions, and anemia. Here, our aim is to characterize significant biomarkers and signaling pathways during the treatment of MM by using bioinformatic methods. The GSE180018 dataset was generated by DNBSEQ-G400 (Homo sapiens). The KEGG and GO analyses showed the biological processes such as " Protein export”, “Protein processing in endoplasmic reticulum”, “Vibrio cholerae infection”, “Fc gamma R-mediated phagocytosis" are mainly affected in FOXM1 KO MM cells. Moreover, we identified the significant genes including CD44, NOTCH1, SELL, RAC2, HSPA8, VAV1, DDIT3, PLK1, HYOU1, ITGAL in FOXM1 KO MM cells. Therefore, our study may provide further guidance for the drug development of MM.

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hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway

Cited by 9 publications

References 55 publications

HSF1 Alleviates Brain Injury by Inhibiting NLRP3-Induced Pyroptosis in a Sepsis Model

HSF1 Alleviates Brain Injury by Inhibiting NLRP3-Induced Pyroptosis in a Sepsis Model

Revealing Molecular Mechanisms of the Bioactive Saponins from Edible Root of Platycodon grandiflorum in Combating Obesity

Identification of biomarkers and signaling pathways during the treatment of multiple myeloma

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