HPLC analysis ofPhyllanthus amarussamples stored in stability chambers under different conditions and study of the effect on quantification of the phytomarkers phyllanthin and hypophyllanthin

Abstract

Summary. Storage of medicinal plants may cause deterioration of the active principles with time, thus, reducing the efficacy of plants. Therefore, quantification of the active principle is essential before using the crude drug. Phyllanthus amarus (PA) contains lignans, namely, phyllanthin and hypophyllanthin, which shows anti-hepatotoxic activity. In this paper, we highlight the effect of storage conditions on the quantification of bioactive markers by high-performance liquid chromatography (HPLC) analysis in the crude plant material of PA. HPLC analysis of crude PA samples stored for certain period at long-term study (LS, 30 °C and 65% RH), accelerated study (AS, 40°C and 75% RH), and real-time study (RT) conditions was carried out using the LiChroCART Purospher® STAR RP-18 endcapped (250 × 4.6 mm, 5 μm) column along with a Purospher STAR RP 18e (4.0 × 4.0 mm, 5 μm) guard column using methanol:water (70:30) at a flow rate of 0.7 mL min −1 with ultraviolet (UV) detection at 220 nm. The HPLC study indicated that PA samples kept under LS condition are rich in lignan contents as compared to the samples stored under AS and RT study conditions. Therefore, PA should be used fresh to get maximum concentration of active lignans or it should be stored under LS conditions up to 6 months.

HPTLC Analysis and Stability Study of Phyllanthin Biomarker in Tablet Formulation

“…One way to preserve the plant products is to dry them in order to conserve their desirable qualities, reduce storage volume, and extend their shelf life. Several analytical procedures involving high performance liquid chromatography (HPLC) [8][9][10][11][12] and highperformance thin-layer chromatography (HPTLC) 13,14 have been described for detection of these lignans. In the present study, we are reporting the stability study of crude plant material of P. Amarus and its effect on quantification of lignan phyllanthin.…”