HPLC Analysis of Organic Acids and Sugars in Tomato Juice

Gancedo, M. Cristina; Luh, B. S.

doi:10.1111/j.1365-2621.1986.tb13881.x

Cited by 83 publications

(26 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sugars (glucose, fructose and sucrose) were analysed by HPLC with a differential refractometer according to the method of Gancedo & Luh (1986). Fresh pepper (30 g) was refluxed with 60 mL of 80% ethanol for 1 h at 80 °C.…”

Section: Methodsmentioning

confidence: 99%

Anti‐oxidant content of different coloured sweet peppers, white, green, yellow, orange and red (Capsicum annuum L.)

Matsufuji

Ishikawa²,

Nunomura³

et al. 2007

Int J of Food Sci Tech

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Anti‐oxidant content of different coloured sweet peppers, white, green, yellow, orange and red (Capsicum annuum L.)

Matsufuji

Ishikawa²,

Nunomura³

et al. 2007

Int J of Food Sci Tech

View full text Add to dashboard Cite

show abstract

“…The extracted solution was acidic, indicating the presence of organic acids from the tomato fruit. Miraculin is considered stable in acidic conditions (Gancendo and Luh 2006;Theerasilp and Kurihara 1988). We adjusted the pH of this extract to 7.2 with 1 M NaOH to allow the surface-exposed histidine residues of miraculin to bind to the nickel-immobilized affinity column.…”

Section: Resultsmentioning

confidence: 99%

“…Miraculin is produced in relatively large amounts in transgenic tomatoes compared to in transgenic strawberries (Sugaya et al 2008), and gene silencing did not occur in the next generation as it did in transgenic lettuce (Sun et al 2006;Yano et al 2010). Moreover, miraculin expressed in tomato fruit is stable due to the acidic pH inside the tomato fruit (Gancendo and Luh 2006;Theerasilp and Kurihara 1988). Additionally, tomatoes can be easily cultured and harvested, with large production yields.…”

mentioning

confidence: 99%

A simple method for purifying undenatured miraculin from transgenic tomato fruit

Duhita

Hiwasa-Tanase

Yoshida

et al. 2011

Plant Biotechnology

View full text Add to dashboard Cite

The sweet taste-modifying protein miraculin has been successfully expressed in the tomato, a high-yield commercial plant (Sun et al. 2007). Previously proposed methods of purifying untagged miraculin from transgenic tomato fruit are laborious and time consuming and do not allow the separation of undenatured and denatured miraculin. Therefore, it is necessary to develop an efficient method of purifying undenatured miraculin from transgenic tomato fruit. In this study, we have demonstrated that the combined use of nickel-immobilized affinity chromatography and ion-exchange chromatography readily provides highly purified undenatured miraculin, as demonstrated by the observation of both a single peak from reverse phase high performance liquid chromatography and a single band from SDS-PAGE.Key words: Miraculin, nickel column, taste-modifying protein, tomato, transgenic plants.Plant Biotechnology 28, 281-286 (2011) DOI: 10.5511/plantbiotechnology.11.0207a Original PaperAbbreviations: HPLC, high performance liquid chromatography; IMAC, immobilized metal affinity chromatography; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, TBS, tris-buffered saline. This article can be found at

show abstract

“…They were chosen among the organic acids (able to act as metal complexing agents) which are known to be present in these vegetables [28][29][30][31][32], together with sucrose and sodium chloride generally added in large amounts to preserving liquids. These components are listed in Table 1 where their solution concentration and pH (adjusted by suitable addition of HClO 4 or NaOH) are also reported.…”

Section: Metal Release Into Canned Synthetic Samplesmentioning

confidence: 99%

A voltammetric approach to an estimate of metal release from tinplate promoted by ligands present in canned vegetables

et al. 2008

View full text Add to dashboard Cite

A linear sweep voltammetric (LSV) approach is proposed for achieving rapid information on metal release from tinned containers into preserved vegetables. Chopped tomato and pea preserves were chosen as prototypes for acid and nearly neutral canned food, respectively. Metal release in these vegetables tinned into both bare and lacquered containers was compared with that found in synthetic samples containing some organic complexing agents (acetic, ascorbic, citric, malic, oxalic, pyroglutamic acids) present in the vegetables considered, thus showing that some of these components affected markedly the metal release in bare cans. These ligands were concomitantly found to also affect LSV profiles recorded in parallel at Sn and Fe electrodes in synthetic samples, causing the starting potential for their oxidation to be lower, the higher the ligand activity of the species considered. The data indicated that metal release and starting potentials for metal discharge are strictly related, so that LSV is able to provide rapid and useful information on the nature of the protective effect (electrochemical or physical) of tin on the steel underlayer, as well as on the extent of Sn and Fe release.

show abstract

HPLC Analysis of Organic Acids and Sugars in Tomato Juice

Cited by 83 publications

References 18 publications

Anti‐oxidant content of different coloured sweet peppers, white, green, yellow, orange and red (Capsicum annuum L.)

Anti‐oxidant content of different coloured sweet peppers, white, green, yellow, orange and red (Capsicum annuum L.)

A simple method for purifying undenatured miraculin from transgenic tomato fruit

A voltammetric approach to an estimate of metal release from tinplate promoted by ligands present in canned vegetables

Contact Info

Product

Resources

About