HPLC analysis of phospholipids by evaporative laser light‐scattering detection

Abstract

High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soybeans subjected to storage at high moisture content. Analytical and preparative separations of phospholipids by normal-phase HPLC were accomplished. Major class frac~ tions were analyzed by transmethylation and capillary column chromatography for fatty acid composition, and by reversephase CA8 HPLC (RP-HPLC) for molecular species composition. The RP-HPLC-ELSD system was limited to the analysis of phosphatidylcholine and phosphatidylethanolamine.

Selective Extraction of Phospholipid Mixtures by Supercritical Carbon Dioxide and Cosolvents

“…Separation was achieved using a linear gradient program consisting of chloroform:tert-butyl methyl ether (750:150 v/v) to methanol:ammonium hydroxide:chloroform (920:70:10 v/v) over 30 min, with an isocratic hold of 10 min. The flow rate was 0.5 mL/min (Abidi, 1994;Mounts et al, 1992). PLFs were dried and redissolved in 3 mL chloroform before HPLC.…”

Determination of surface-bound hydroxypropylcellulose (HPC) on drug particles in colloidal dispersions using size exclusion chromatography: A comparison of ELS and RI detection

“…Recently, applications of evaporative light scattering detectors (ELS) have increased dramatically due to the possibility of replacing the RI detector and the greater flexibility of ELS [21,22]. ELS detection has been used to successfully quantitate synthetic polymers [23], carbohydrates [19,24], fats and fatty acid esters [25,26], triglycerides [27], and steroids [28]. However, even though both detection technologies are available, no methods have been reported using size exclusion chromatography wherein ELS and RI detection have been compared for quantitation of HPC.…”

A kinetic study of phospholipid extraction by degumming process in sunflower seed oil

“…During the refining process (degumming), phospholipids are eliminated by thermal treatment with water (hydratable phospholipids, HP) and other degumming agents such as phosphoric acid, citric acid or acid mixtures (nonhydratable phospholipids, NHP). According to research performed on soybean oils, the presence of NHP is influenced by factors such as temperature and moisture during the storage of seeds, and cellular damage, which would facilitate the action of phospholipase D, yielding higher levels of phosphatidic acid (2).…”