HPLC analysis on separation of BSA from dilute solution

Mukhopadhyay, Goutam

doi:10.1530/endoabs.31.p40

Cited by 1 publication

(1 citation statement)

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“…RP-HPLC, as presented in Figure 8, was employed to quantitate the in vitro BSA/endostatin release from the BSA/endostatin-loaded nanoparticles as shown in Figure 11. BSA elute at 2.216 minutes over a run time of 6 minutes and result corroborates to previous report of Mukhopadhyay [38] and Mukhopadhyay et al [39].…”

Section: Nanoparticle Swelling Behaviour and In Vitro Drugsupporting

confidence: 91%

Design and Characterization of Endostatin-Loaded Nanoparticles for In Vitro Antiangiogenesis in Squamous Cell Carcinoma

Adeyemi

Choonara

Kumar

et al. 2017

Journal of Nanomaterials

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The aim of this study is to effectively enhance antitumor activities of endostatin by preparing polymeric nanocarriers. NMR and FT-IR spectra confirmed the successful grafting of the CHT-g-PEI and CHT-g-PEI-PEG-NH 2 conjugates. SEM micrographs confirmed the shape of endostatin-loaded nanoparticles to be spherical while both TEM and zeta size results showed nanoparticle's average size to be 100.6 nm having a positively charged surface with zeta potential of 7.95 mV. The concentrations of CHT and TPP as well as the changing pH conditions account for the increased swelling pattern of endostatin-loaded nanoparticles and influenced endostatin release in vitro. PEI increased the overall amine protonation while PEG aggravated endostatin encapsulation and release. Nanoparticles swell and release endostatin at acidic tumor pH of 6.8 compared to physiological pH of 7.4. The native CHT-g-PEI-PEG-NH 2 conjugate showed high cytocompatibility above 80% cell viability across tested formulations. Endostatin-loaded nanoparticles showed a significant reduction in cell viability across tested formulations, with 5.32% cell death at 125 g/mL and 13.36% at 250 g/mL following 24 hours' incubation period. Interestingly, more than a fourfold (61.68%) increment in cytotoxicity was observed at nanoparticle concentration of 1000 g/mL. It was concluded that CHT-g-PEI-PEG-NH 2 is an effective cargo for endostatin delivery with antiangiogenic effect in squamous cell carcinoma.

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Section: Nanoparticle Swelling Behaviour and In Vitro Drugsupporting

confidence: 91%