HPLC and its Essential Role in the Analysis of Tricyclic Antidepressants in Biological Samples

Samanidou, Victoria; Nika, Maria; Papadoyannis, Ioannis N.

doi:10.1016/b978-0-12-803961-8.50011-7

Cited by 2 publications

(1 citation statement)

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“…Several methods have been reported for their estimation in bulk and dosage forms, such as UV-Vis. spectrophotometry [2,3], extractive photometry [4], stability-indicating high-performance liquid chromatography (HPLC) [5], HPLC [6][7][8], and spectrofluorimetry with ion pair complexation [9,10]. The bio-analytical methods developed for the estimation of doxepin and its metabolites in spiked human plasma and urine include liquid chromatography with tandem mass spectrometry [11] and HPLC [12,13], respectively.…”

Section: Introductionmentioning

confidence: 99%

Determination of doxepin hydrochloride in spiked human plasma and capsule after derivatization with eosin Y using resonance Rayleigh scattering, second‐order scattering and frequency doubling scattering methods

et al. 2023

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Doxepin hydrochloride (DOX) is a tricyclic antidepressant drug. Three sensitive spectrofluorimetric methods, namely resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS) and second-order scattering (SOS), were developed and validated for their estimation of doxepin in spiked human plasma and formulation using liquid-liquid extraction method through the formation of an ion pair complex with eosin Y at a pH of 4.5. Various factors affecting fluorescence intensity were optimized, and the reaction kinetics was determined using the Arrhenius equation method. Different scattering methods such as RRS, FDS and SOS were developed at maximum scattering wavelengths λ ex /λ em = 567/567 nm for RRS, 720/360 nm for SOS and 260/520 nm for FDS, respectively. The methods exhibited high sensitivities, and the detection limits for DOX were found to be 0.82, 1.20 and 1.03 ng/ml for RRS, FDS and SOS methods, respectively. The FDS method exhibited the highest sensitivity. The methods were validated using the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use guidelines and applied to determine DOX in capsule and spiked human plasma samples.

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