HPLC-DAD analysis of Hyssopus Cuspidatus Boriss extract and mensuration of its antioxygenation property

Abstract

Background Hyssopus cuspidatus Boriss has been used as an important ethnomedicinal plant for long to eliminate phlegm, relieve cough and as well as having antibacterial, antioxygenation, and antitumor activities. In this study, the polyphenol contents, flavonoid contents, free radical scavenging assay and animal antioxygenation property assay of ethanol extract of H. cuspidatus were measured. Methods This study determined the total polyphenol and flavonoid contents in H. cuspidatus by UV-VIS. Caffeic, ferulic, and rosmarinic acids were measured using HPLC-DAD. Free radical scavenging assay of H. cuspidatus was studied by colorimetric method. Animal antioxygenation property assay of H. cuspidatus was studied with mice by biochemical assay kits. Results The total polyphenol and flavonoid contents of H. cuspidatus in 2017, 2018, 2019 were determined and the contents of H. cuspidatus in 2019 was the highest. In addition, rosmarinic acid was the phenolic acid with the highest content in H. cuspidatus . Compared with those of DPPH free radical, hydroxyl free radical, and superoxide anion free radical, the scavenging ability of H. cuspidatus of ABTS free radical was stronger, the average IC 50 value was 0.0245 mg/mL. In animal antioxygenation property experiment, the model group was successfully established with decreased activities of SOD, CAT, and GSH-px and increased content of MDA. The ethanol extract of H. cuspidatus increased the activities of SOD, CAT, and GSH-px and reduced the content of MDA. Each group of samples and the ascorbic acid positive control group showed significant differences in the results of free radical scavenging and animal antioxygenation property experiments ( P < 0.05). Conclusions These results suggest that H. cuspidatus exerts an antioxygenation property, which can be attributed to the contents of total polyphenol and flavonoid. Given its strong antioxygenation property, H. cuspidatus can be used as a new natural antioxidant in food preservation and disease treatment.

Simultaneous Determination of the Five Constituents in Maiwei Dihuang Pills by the HPLC-DAD Method

“…However, the composition of traditional Chinese medicine is complex, and it is not enough to control the quality of the whole preparation only by the two. Highperformance liquid chromatography-diode array detector (HPLC-DAD) is an effective method for chemical analysis, which has been well established in a previous study [10,11].…”

Renal Protective Effects of Inonotus obliquus on High-Fat Diet/Streptozotocin-Induced Diabetic Kidney Disease Rats: Biochemical, Color Doppler Ultrasound and Histopathological Evidence

“…The total polysaccharide contents (TPCCs), total polyphenol contents (TPCs) and total flavonoid contents (TFCs) in the above extracts were analyzed according to current literatures ( Song et al, 2016 ; Liao et al, 2020 ; Zhao et al, 2020 ; Fanali et al, 2021 ), and described in brief as follows.…”

“…TFCs were determined with UV colorimetric assay ( Zhao et al, 2020 ). In brief, 0.5 ml of chaga extracts was added to 1 ml of sodium nitrite, and allowed to sit for 6 min, 1 ml of 10% aluminum nitrate was then added, and allowed to sit for another 6 min.…”

Exploration of the Molecular Mechanisms of Hyssopus cuspidatus Boriss Treatment of Asthma in an mRNA-miRNA Network via Bioinformatics Analysis

“…H. cuspidatus contains a variety of chemical components, including volatile oils, flavonoids, organic acids, and lipids [ 10 , 11 ]. In recent years, H. cuspidatus and its extractions have played an important role in the treatment of asthma [ 12 , 13 ]. Pharmacological studies have demonstrated that H. cuspidatus can treat asthma through its anti-inflammatory and antibacterial effects [ 14 ].…”