HPLC-DAD characterisation of phenolic compounds from Andean oca (Oxalis tuberosa Mol.) tubers and their contribution to the antioxidant capacity

Chirinos, Rosana; Betalleluz-Pallardel, Indira; Huamán, Anabel; Arbizu, Carlos I.; Pedreschi, Romina; Campos, David

doi:10.1016/j.foodchem.2008.08.015

Cited by 71 publications

(41 citation statements)

References 21 publications

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“…The yield of this hydroethanolic extract was 12.0 g per 100 g of crude plant material. To characterize the qualitative chemical profile, this extract was initially analyzed via TLC (Stahl, 1969;Wagner & Bladt, 1996;Chirinos et al, 2009). Dried TLC plates were sprayed with specific reagent and heated to observe the color reaction.…”

Section: Resultsmentioning

confidence: 99%

“…The free flavonoid aglycones of flavonoid-Oglycosides were released by acid hydrolysis as follows: 50 mg of lyophilized extract of S. guianensis were dissolved in 4 mL of solution 10% (v/v) H 2 SO 4 , and heated in boiling water for 1 h (Chirinos et al, 2009). After cooling, the reaction mixture was neutralized with saturated aqueous sodium carbonate and filtered under reduced pressure.…”

Section: Hydrolysis Experimentsmentioning

confidence: 99%

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Chemical composition of hydroethanolic extracts from Siparuna guianensis, medicinal plant used as anxiolytics in Amazon region

Negri¹,

Santi²,

Tabach³

2012

Rev. bras. farmacogn.

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Siparuna guianensis Aubl., Siparunaceae, is used as anxiolytic plants in folk medicine by South-American indians, "caboclos" and river-dwellers. This work focused the evaluation of phenolic composition of hydroethanolic extract of S. guianensis through HPLC-DAD-ESI/MS/MS. The constituents exhibited protonated, deprotonated and sodiated molecules and the MS/MS fragmentation of protonated, deprotonated and sodiated molecules provided product ions with rich structural information. Vicenin-2 (apigenin-6,8-di-C-glucoside) was the main constituent found in S. guianensis together quercetin-3,7-di-O-rhamnoside and kaempferol-3,7-di-O-rhamnoside. A commercial extract of Passiflora incarnata (Phytomedicine) was used as surrogate standard and also was analyzed through HPLC-DAD-ESI/ MS/MS, showing flavones C-glycosides as constituents, among them, vicenin-2 and vitexin. The main constituent was vitexin. Flavonols triglycosides were also found in low content in S. guianensis and were tentatively characterized as quercetin-3-O-rutinoside-7-O-rhamnoside, quercetin-3-O-pentosyl-pentoside-7-O-rhamnoside and kaempferol-3-O-pentosyl-pentoside-7-O-rhamnoside. Apigenin and kaempferol derivatives had been reported as anxiolytic agents. Flavonoids present in this extract were correlated with flavonoids reported as anxiolytics.

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Section: Resultsmentioning

confidence: 99%

Section: Hydrolysis Experimentsmentioning

confidence: 99%

Chemical composition of hydroethanolic extracts from Siparuna guianensis, medicinal plant used as anxiolytics in Amazon region

Negri¹,

Santi²,

Tabach³

2012

Rev. bras. farmacogn.

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“…Igualmente, el grupo de la Facultad de Industrias Alimentarias conformado por el Dr. David Campos, la Dra. Rosana Chirinos -ambos profesores del PDCIB -y otros profesores realizan investigaciones muy importantes sobre metabolitos de plantas nativas generando grandes oportunidades para la utilización económica de la biodiversidad vegetal (Chirinos et al 2007(Chirinos et al , 2008(Chirinos et al , 2009(Chirinos et al , 2010.…”

Section: Algunos Logros En Biotecnologíaunclassified

El camino de la biotecnología en la Universidad Nacional Agraria La Molina, Perú

Gutiérrez-Correa

2013

Rev peru biol

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COMENTARIO Presentado:26/11/2012 Aceptado:15/12/2012 Publicado online: 15/01/2013 ResumenSe realiza un relato histórico sobre los cincuenta años que tiene la carrera de biología en la Universidad Nacional Agraria La Molina y la gestación, inicio, desarrollo y maduración de la biotecnología en esta universidad. Se describen algunos pormenores de las tres grandes estructuraciones curriculares que finalmente derivan al establecimiento del área de biotecnología en el pregrado y en el doctorado así como a los logros alcanzados. El desarrollo de la biotecnología en la UNALM ha influenciado el desarrollo de esta área en el Perú no solo en las adaptaciones curriculares que se han producido en varias universidades del país sino también en las investigaciones biotecnológicas que se llevan a cabo en universidades e institutos de investigación en las diferentes regiones del país. En este devenir histórico se concluye que el balance es muy positivo y que la mejor corriente epistemológica es la que nos lleva mediante la investigación a encontrar verdades usables en la solución de los problemas del país y, también por qué no, del planeta.Palabras clave: historia de la biología en el Perú; biotecnología; universidad; curriculum. AbstractIn this paper, I make a historical account of the fifty years that have the degree in biology at the Universidad Nacional Agraria La Molina and gestation, and the beginning, development and maturation of biotechnology in this university. We describe some details of the three most important curricular modifications that led to the establishment of the biotechnology area in the curricula of undergraduate and doctoral programs, as well as their main achievements. The development of biotechnology at the UNALM has influenced the development of this area in Peru not only in the curricular changes that have occurred in several Peruvian universities, but also in the biotechnology research being carried out in many regions of the country. In this historical process, I conclude that the balance is positive and that the best epistemology stream is that which leads through research to find truths usable for solving the problems of Peru and also why not the planet.Keywords: history of biology in Peru; biotechnology; university; curriculum. Amicus Plato sed magis amica veritas -AristótelesCaminante, no hay camino, se hace camino al andar -Antonio Machado IntroducciónHan transcurrido cincuenta años desde que en 1962 se creó la Carrera de Biología en la Facultad de Ciencias de la Universidad Nacional Agraria La Molina (UNALM). Mi relación con la carrera lleva 44 años, primero como estudiante desde 1968 y luego como profesor desde 1975 (37 años) por lo que me ha tocado ser parte de su desarrollo histórico, incluyendo el periodo 1988 a 1992 como presidente de la Comisión de Currículo de la Facultad de Ciencias encargada de la última gran reestructuración curricular. Es indudable que relatar la historia es una tarea extremadamente difícil por el inevitable sesgo del relator, aunque el respetar los hechos y las evi...

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“…The composition of solvents and gradient elution conditions were applied as previously described by Chirinos et al, (2009) with some modifications. The mobile phase composed of wateracetic acid (94:6, v/v, pH 2.27) (A) and acetonitrile (B).…”

Section: Hplc-dadanalysis Of Phenolic Compoundsmentioning

confidence: 99%

“…The phenolic compounds present in the samples were characterized according to their UV-vis spectra and identified by their retention times in comparison with those of commercial standards. For the quantitative analysis of phenolic compounds, a calibration curve was made by the injection of different concentrations of gallic acid, protocatechuic acid, hydroxybenzoic acid, vanillic acid, p-coumaric acid, and cinnamic acid standards (Chirinos et al, 2009). …”

Section: Hplc-dadanalysis Of Phenolic Compoundsmentioning

confidence: 99%

Antioxidant activity of phenolic extracts from kenaf (<i>Hibiscus cannabinus</i>) seedcake.

Mariod¹,

Ibrahim²,

Ismail³

et al. 2012

Grasas y Aceites

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The antioxidant activities of kenaf seedcake methanolic extract (CME) and fractions obtained from it using ethyl acetate (EAF), hexane (HF) and water (WF) were investigated. Total phenolic contents were 64.5, 36.1, 31.3 and 14.6 mg gallic acid equivalent/g dry weight in EAF, CME, WF, and HF, respectively. Kenaf seedcake extract/fractions showed inhibitory activity of β-carotene bleaching and corn oil oxidation. Also, the extract/fractions were scavenged for the 1,1-diphenyl-2-picrylhydrazyl radical. The EAF extract showed the highest DPPH radical scavenging activity followed by the CME, WF and HF extracts. Therefore, the rich phenolic fractions of kenaf seedcake may represent a potential source of natural antioxidants. The predominant phenolic compounds identified by HPLC-DAD in CME and HF extracts were gallic, (+)-catechin, chlorogenic, hydroxybenzoic, syringic, and vanillin.

Se ha investigado la actividad antioxidante de extractos metanólicos procedentes de tortas de semillas de kenaf (CME) y de las fracciones obtenidas usando acetato de etilo (EAF), hexano (HF) y agua (WF). Los contenidos fenólicos totales fueron 64,5, 36,1, 31,3 y 14,6 mg de equivalente de ácido gálico/g de peso seco, en EAF, CME, WF, y HF, respectivamente. Los extractos/fracciones de semillas de kenaf mostraron actividad inhibitoria de blanqueo del β-caroteno y oxidación del aceite de maíz. Además, los extractos/fracciones fueron captadores de radicales del 1,1-difenil-2-picrilhidrazil. El extracto EAF mostró la mayor actividad captadora de radicales de DPPH seguido por los extractos de CME, WF y HF. Por lo tanto, las fracciones ricas en fenoles de las tortas de semilla de kenaf pueden representar una fuente potencial de antioxidantes naturales. Los compuestos fenólicos predominantes identificados mediante HPLC-DAD en extractos de CME y HF fueron gálico, (+)-catequina, ácido clorogénico, hidroxibenzoico, siríngico, y vainillina

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HPLC-DAD characterisation of phenolic compounds from Andean oca (Oxalis tuberosa Mol.) tubers and their contribution to the antioxidant capacity

Cited by 71 publications

References 21 publications

Chemical composition of hydroethanolic extracts from Siparuna guianensis, medicinal plant used as anxiolytics in Amazon region

Chemical composition of hydroethanolic extracts from Siparuna guianensis, medicinal plant used as anxiolytics in Amazon region

El camino de la biotecnología en la Universidad Nacional Agraria La Molina, Perú

Antioxidant activity of phenolic extracts from kenaf (<i>Hibiscus cannabinus</i>) seedcake.

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