HPLC-DAD-ESI-MS/MS screening of phytochemical compounds and the bioactive properties of different plant parts of<i>Zizyphus lotus</i>(L.) Desf.

Rached, Wahiba; Barros, Lillian; Ziani, Borhane E.C.; Bennaceur, Malika; Calhelha, Ricardo C.; Heleno, Sandrina A.; Marouf, Abderrazak; Ferreira, Isabel C.F.R.

doi:10.1039/c9fo01423c

Cited by 19 publications

(15 citation statements)

References 57 publications

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“…In contrary, it exhibited a significant activity against MCF-7 cells. These findings can be due to the main bioactive compounds contained in Zls extract that may include flavonoids, tannins, alkaloids, terpenoids, and saponins as observed previously from other jujube species and from other part of Zizyphus lotus which showed a strong antiproliferative activity against HepG 2 and MCF-7 cells [21,74].…”

Section: Anti-proliferative Activity Of Zls Extractsupporting

confidence: 64%

“…The effective cytotoxicity toward hepatocellular HepG 2 and beast MCF-7 could be related to the presence of the some secondary metabolites, some of them are considered the major class of jujube polyphenols, represented from 89 to 94% of the total phenolic contents mainly flavan-3-ols such as monomer (as (-)-epicatechin, gallocatechin gallate, and (+)-catechin), dimer (procyanidine B2), and polymeric proanthocyanidins in Zls, which were applicated in the inhibition of cell proliferation in different cancer types [47]. Quercetin-3-O-rutinoside found in other parts of Z. lotus, which represents 50% of jujube flavonoids offers a plausible explanation of the observed cytotoxicity [74].…”

Section: Anti-proliferative Activity Of Zls Extractmentioning

confidence: 99%

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Ultrasound Assisted Extraction of Phenolic Compounds from a Jujube By-Product with Valuable Bioactivities

et al. 2020

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Jujube plant is a potential source of polyphenols with biological propreties. The purpose of this study was to investigate the application of ultrasound technique for extracting phenolic compounds (TPC) from seeds of Zizyphus lotus under optimization conditions based on response surface methodology. A maximum TPC, total flavonoids content (TFC), and total condensed tannins content (TTC) of 2383.10 ± 0.87 mg GAE/100g, 486.50 ± 0.38 mg QE/100g and 15,787.10 ± 0.10 mg CE/100g, respectively obtained under ethanol concentration 50.16%, sonication temperature 29.01 °C, sonication time 15.94 min and solvent-to-solid ratio 34.10:1 mL/g. The optimized extract was then evaluated for its antioxidant, antiacethylcholinesterase, antihypercholesterolemia, and antiproliferative activities. The results showed that ultrasound method is a green and safe method that can be used to effectively extract TPC from jujube seeds. The biological activity of Zizyphus extract exhibited a very good antioxidant against DPPH (EC50 = 0.39 µg/mL) and FRAP (1670.42 ± 6.5 mg/100 g). Additionally, it possesses acetylcholinesterase (AChE) inhibitory effect (IC50 = 0.93 ± 0.01 mg/mL) and HMGR inhibition (45.41%) using 100 µg/mL. The extract significantly inhibits cell proliferation on the MCF-7 and HepG2 tumor cell lines with an IC50 values of <0.05 and 3 ± 0.55 mg/mL, respectively. Therefore, the ultrasound method can be considered a method for obtaining a significant anticancer activity with respect to the lines and therefore makes it possible to recover a maximum of phenolic compounds in less time with an AChE and HMGR inhibitory activity. Thus, it can be suggested that Zls extract is a promising fruit for the development of supplementary dietary due to its potential behaviour as nutraceutical.

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Section: Anti-proliferative Activity Of Zls Extractsupporting

confidence: 64%

Section: Anti-proliferative Activity Of Zls Extractmentioning

confidence: 99%

Ultrasound Assisted Extraction of Phenolic Compounds from a Jujube By-Product with Valuable Bioactivities

et al. 2020

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“…According to EC 50 values on SH-SY5Y after 24 and 48 h of treatments, the dried root extracts of petroleum ether (DR-PEE) and dichloromethane (DR-DE) were selected and subsequent investigations were pursued. As reported by Rached et al [31], the root barks are always expressing a potent cytotoxic effect. Consistent cytotoxic activities were also observed by testing the root bark extracts on hepatocellular HepG2 (48.3 µg/mL), breast MCF-7 (74 µg/mL) and cervical HeLa (69 µg/mL) [31].…”

Section: Discussionmentioning

confidence: 72%

“…As reported by Rached et al [31], the root barks are always expressing a potent cytotoxic effect. Consistent cytotoxic activities were also observed by testing the root bark extracts on hepatocellular HepG2 (48.3 µg/mL), breast MCF-7 (74 µg/mL) and cervical HeLa (69 µg/mL) [31].…”

Section: Discussionmentioning

confidence: 72%

Chemical Composition and Biological Activities of Tunisian Ziziphus lotus Extracts: Evaluation of Drying Effect, Solvent Extraction, and Extracted Plant Parts

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Garzoli

Masci

et al. 2021

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The Tunisian Ziziphus lotus plant was investigated to determine its phytoconstituents and evaluate its biological activities. In particular, the GC/MS technique was used to describe the chemical composition of Z. lotus active extracts and fractions. Among the obtained extracts, the yields of the dried root methanolic extract (29.80%) and the fruit aqueous extract (48.00%) were the highest ones. The dried root methanolic extract exhibited the highest amount in the total phenolics (186.44 ± 0.26 mg GAE/g DW), total flavonoids (102.50 ± 3.53 mg QE/g DW), and tannins (60.714 ± 2.2 mg catechin/g DW). The root aqueous extracts revealed the highest antioxidant activity with an IC50 of 8.96 ± 0.38 mg/L and 16.46 ± 0.60 mg/L for the ABTS•+ and DPPH• assays, respectively. The total antioxidant capacity was accorded to the methanolic extract of the dried roots with a value of 304.07 ± 1.11 µg AAE/mg. The drying process was found to improve the qualitative and quantitative properties of the Z. lotus extracts. The evaluation of the cytotoxic activity against the SH-SY5Y cell line was carried out using MTT assay. The petroleum ether and dichloromethane extracts of the dried roots showed relevant cytotoxic activities. The thin layer chromatography and the GC-MS/GC-FID analysis led to the identification of the 13-epimanool as a potent cytotoxic compound.

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“…An increasing number of studies have identified a variety of bioactive secondary metabolites in Z. lotus, particularly phenolic compounds, which have been verified to be present in leaves, branches, and root and stem barks [7,8]. In addition, a vast number of biological properties have been attributed to Z. lotus polar extracts from different morphological parts, namely, antioxidant [8][9][10], antibacterial [8], anti-ulcerogenic [10], anti-inflammatory [5,8], analgesic [5], antidiabetic [9] and antispasmodic [11] activities.…”

Section: Introductionmentioning

confidence: 99%

Chemical Profile of Lipophilic Fractions of Different Parts of Zizyphus lotus L. by GC-MS and Evaluation of Their Antiproliferative and Antibacterial Activities

et al. 2022

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Zizyphus lotus L. is a perennial shrub particularly used in Algerian folk medicine, but little is known concerning the lipophilic compounds in the most frequently used parts, namely, root bark, pulp, leaves and seeds, which are associated with health benefits. In this vein, the lipophilic fractions of these morphological parts of Z. lotus from Morocco were studied by gas chromatography–mass spectrometry (GC–MS), and their antiproliferative and antimicrobial activities were evaluated. GC–MS analysis allowed the identification and quantification of 99 lipophilic compounds, including fatty acids, long-chain aliphatic alcohols, pentacyclic triterpenic compounds, sterols, monoglycerides, aromatic compounds and other minor components. Lipophilic extracts of pulp, leaves and seeds were revealed to be mainly composed of fatty acids, representing 54.3–88.6% of the total compounds detected. The leaves and seeds were particularly rich in unsaturated fatty acids, namely, (9Z,12Z)-octadeca-9,12-dienoic acid (2431 mg kg−1 of dry weight) and (9Z)-octadec-9-enoic acid (6255 mg kg−1 of dry weight). In contrast, root bark contained a high content of pentacyclic triterpenic compounds, particularly betulinic acid, accounting for 9838 mg kg−1 of dry weight. Root bark extract showed promising antiproliferative activity against a triple-negative breast cancer cell line, MDA-MB-231, with a half-maximal inhibitory concentration (IC50) = 4.23 ± 0.18 µg mL−1 of extract. Leaf extract displayed interesting antimicrobial activity against Escherichia coli, methicillin-sensitive Staphylococcus aureus and Staphylococcus epidermis, presenting minimum inhibitory concentration (MIC) values from 1024 to 2048 µg mL−1 of extract. Our results demonstrate that Zizyphus lotus L. is a source of promising bioactive components, which can be exploited as natural ingredients in pharmaceutical formulations.

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HPLC-DAD-ESI-MS/MS screening of phytochemical compounds and the bioactive properties of different plant parts ofZizyphus lotus(L.) Desf.

Abstract: Zizyphus lotus L. (Desf.) presented flavonoids and secoiridoids derivatives and revealed to have cytotoxicity, anti-inflammatory and antimicrobial potential.

Cited by 19 publications

References 57 publications

Ultrasound Assisted Extraction of Phenolic Compounds from a Jujube By-Product with Valuable Bioactivities

Ultrasound Assisted Extraction of Phenolic Compounds from a Jujube By-Product with Valuable Bioactivities

Chemical Composition and Biological Activities of Tunisian Ziziphus lotus Extracts: Evaluation of Drying Effect, Solvent Extraction, and Extracted Plant Parts

Chemical Profile of Lipophilic Fractions of Different Parts of Zizyphus lotus L. by GC-MS and Evaluation of Their Antiproliferative and Antibacterial Activities

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