HPLC-DAD method for simultaneous determination of gallic acid, catechins, and methylxanthines and its application in quantitative analysis and origin identification of green tea

Jakabová, Silvia; Árvay, Július; Šnirc, Marek; Lakatošová, Jana; Ondejčíková, Alena; Golian, Jozef

doi:10.1016/j.heliyon.2024.e35819

Heliyon

2024

DOI: 10.1016/j.heliyon.2024.e35819

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HPLC-DAD method for simultaneous determination of gallic acid, catechins, and methylxanthines and its application in quantitative analysis and origin identification of green tea

Silvia Jakabová,

Július Árvay,

Marek Šnirc

et al.

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2024

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Cited by 2 publications

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Paper‐Based Device for Phenolic Content Determination in Tea Extracts

Silver,

Noviana,

Ash Shiddiq

et al. 2024

Phytochemical Analysis

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IntroductionPhenolic compounds garner interest in developing medicines, nutraceuticals, and cosmeceuticals based on natural products. The quantity of phenolic compounds in a sample is commonly determined via spectrophotometry; however, this instrumented technique is relatively laborious and time consuming and requires a large amount of reagents.ObjectiveThis work aimed to develop a simple, point‐of‐need colorimetric sensor to rapidly determine total phenolic content (TPC) in tea extracts.MethodologyWe developed a radial paper‐based analytical device (PAD) for TPC determination based on the established colorimetric reaction between the Folin–Ciocâlteu reagent and phenols. The PAD was designed to enable quantitative (with image capturing device and color processing software) and semiquantitative (using a color palette reference card) determinations. Analytical performance and stability of the PAD were evaluated based on the color responses.ResultsThe PAD was successfully applied for the determination of phenolics in tea extracts obtained using several polar protic solvents, including water, methanol, and ethanol, with satisfactory accuracy (recovery of 95.5%–104%, 110%–116%, and 104%–110%, respectively) and precision (RSD < 9%). The obtained TPC values also agreed with those from visible spectrophotometry. Semiquantitative determination using the color reference card with three categories of TPC level (i.e., 0–100, 100–500, and 500–1000 mg gallic acid equivalent/L) provided > 95% accuracy. The devices were the most stable when stored at 4°C in a light‐protected, vacuum‐sealed container. The proposed PAD is promising for simple, rapid (~10–20 min), and accurate estimation of TPC in plant extracts.

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Paper‐Based Device for Phenolic Content Determination in Tea Extracts

Silver,

Noviana,

Ash Shiddiq

et al. 2024

Phytochemical Analysis

View full text Add to dashboard Cite

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Natural antioxidants: A comprehensive elucidation of their sources, mechanisms, and applications in health

Chibuye,

Singh,

Ramasamy

et al. 2024

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HPLC-DAD method for simultaneous determination of gallic acid, catechins, and methylxanthines and its application in quantitative analysis and origin identification of green tea

Cited by 2 publications

References 51 publications

Paper‐Based Device for Phenolic Content Determination in Tea Extracts

Paper‐Based Device for Phenolic Content Determination in Tea Extracts

Natural antioxidants: A comprehensive elucidation of their sources, mechanisms, and applications in health

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