HPLC–DAD Method for the Quantification of Carbamazepine, Oxcarbazepine and their Active Metabolites in HepaRG Cell Culture Samples

Ferreira, Ana; Rodrigues, Márcio; Falcão, Amı́lcar; Alves, Gilberto

doi:10.1007/s10337-016-3063-7

Cited by 10 publications

(2 citation statements)

References 53 publications

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“…However, in order to perform a successful TDM using the quantitative determination of specific drugs in body fluids, sensitive and selective analytical methods are needed [1,4]. Numerous methods have been reported for the analysis of CBZ in biological samples, using RP-HPLC coupled with UV or MS detection and C18 [5,8,11,18,20,25,27], C8 [9] or C14 [29] stationary phases. Other methods include HPTLC [21] or immunoassay [19] techniques.…”

Section: Introductionmentioning

confidence: 99%

Development of a High Performance Chromatographic Method for Therapeutic Drug Monitoring of Carbamazepine Based on an Experimental Design Approach

Tuchila¹

2018

FARMACIA

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An isocratic simple and rapid HPLC-UV method has been developed and validated for the determination of carbamazepine (CBZ) in human plasma, using nitrazepam (NTZ) as internal standard. Separation was carried out on a C18 column with a mobile phase composed of water:acetonitrile (55:45 v/v), delivered at a flow rate of 1 mL/min. Liquid-liquid extraction with ethyl acetate was used for the isolation of the analytes from plasma. Development of the method was based on a design of experiments (DOE) approach. A 2 3 full factorial design with central points was used in order to select the experimental parameters, while considering peak asymmetry, resolution between CBZ and the internal standard and retention time as responses. Overall, optimization of the analysis parameters was performed using Derringer's desirability function and overlay contour plots. The method was fully validated in the concentration range of 0.1 -20 µg/mL, which included the entire therapeutic range of CBZ (4 -12 µg/mL). The method is simple, rapid (with a total analysis time of 5 minutes) and can be applied in routine clinical investigations. RezumatA fost dezvoltată și validată o nouă metodă lichid cromatografică pentru determinarea cantitativă a carbamazepinei (CBZ) din plasma umană, utilizând nitrazepamul ca standard intern. Separarea a fost realizată pe o coloană de tip C18, utilizând o fază mobilă conținând un amestec de apă și acetonitril, în raport volumic 55:45. Debitul fazei mobile a fost de 1 mL/min. Izolarea analiților din probele de plasmă s-a realizat prin extracție lichid-lichid, folosind acetatul de etil drept solvent de extracție. Dezvoltarea metodei a fost realizată utilizând tehnici de design experimental. A fost utilizat un design de tip factorial, cu trei factori la 2 nivele (2 3 ), considerându-se ca și răspunsuri asimetria picurilor, rezoluția între CBZ și standardul intern, precum și timpul de retenție al analiților. Pentru optimizarea parametrilor, s-au utilizat gradul de dezirabilitate și graficele de contur suprapuse. Metoda a fost validată pe un interval de concentrații între 0,1 și 20 µg/mL, care include zona terapeutică a CBZ (4 -12 µg/mL). Metoda este simplă, rapidă (cu un timp total de analiză de 5 min) și poate fi aplicată în monitorizarea terapeutică a CBZ.

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Section: Introductionmentioning

confidence: 99%

Development of a High Performance Chromatographic Method for Therapeutic Drug Monitoring of Carbamazepine Based on an Experimental Design Approach

Tuchila¹

2018

FARMACIA

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“…Carbamazepine is official in british pharmacopeia 2 as it was determined by liquid chrpomatographic (LC) method. Various techniques have been reported for the assay of CBMZ in pharmaceutical dosage forms and biological fluids, including high-performance liquid chromatography (HPLC) with ultra violet or photo diode detection [3][4][5][6][7][8][9][10][11][12] , high-performance thin layer chromatography (HPTLC) 13 , LC-mass spectrometry methods [14][15][16] , fluorescence polarization assay (FPA) 17,18 , gas chromatography with mass spectrometry [19][20][21] , micellar electro kinetic capillary chromatography (MECC) 22 , chemiluminescence 23,24 , spectrofluorimetry 25 , FT-Raman spectroscopy 26 , flow-injection 27 and spectrophotometry [28][29][30][31][32][33][34][35][36][37] have been reported for the detection of CBMZ and its metabolites.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Rapid Stability-Indicating HPLC Method for Determination of Carbamazepine in Pure and Dosage Forms

2016

Chem Sci Trans

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A new, simple, rapid and accurate stability-indicating HPLC method was developed and validated for quantitative determination of carbamazepine (CBMZ) in pure and dosage forms. An isocratic HPLC method, using a C 18 reversed phase column (150 mm x4.6 mm i.d., particle size 5 µm) with isocratic binary mobile phase consisting of methanol and water (70:30,V/V), was investigated to separate the drug from its stress degradation products. The flow rate was 1.5 mL min-1 at ambient temperature and photo diode array (PDA) detector was used at 285 nm for detection. The elution time of CBMZ was found to be 2.324±0.003 minutes. The developed method was validated for system suitability, linearity, accuracy, precision, limits of detection and quantitation, specificity, stability and robustness. Stability tests were done through exposure of the analyte solution for five different stress conditions: Reflux with 1.0 mol L-1 hydrochloric acid (HCl), reflux with 1.0 mol L-1 sodium hydroxide (NaOH), reflux with 30% hydrogen peroxide (H 2 O 2), exposure to ultra violet radiation (UV) radiation and heating. The calibration curve was found to be linear with the equationy=0.19677x-0.306, with a correlation coefficient of (R 2 =0.9999) over a concentration range of 2.0-24 µgmL-1. The limits of detection and quantification were 0.02 and 0.062 µgmL-1 , respectively. The recovery value of this method is 99.80% and the reproducibility is with in 1.23.

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Influence of the dual combination of silymarin and (-)-epigallocatechin gallate, natural dietary flavonoids, on the pharmacokinetics of oxcarbazepine in rats

Ferreira

Rodrigues

Marques

et al. 2017

Food and Chemical Toxicology

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HPLC–DAD Method for the Quantification of Carbamazepine, Oxcarbazepine and their Active Metabolites in HepaRG Cell Culture Samples

Abstract: in HepaRG cells involving these antiepileptic drugs (CBZ and OXC) and their main metabolites.

Cited by 10 publications

References 53 publications

Development of a High Performance Chromatographic Method for Therapeutic Drug Monitoring of Carbamazepine Based on an Experimental Design Approach

Development of a High Performance Chromatographic Method for Therapeutic Drug Monitoring of Carbamazepine Based on an Experimental Design Approach

Development and Validation of a Rapid Stability-Indicating HPLC Method for Determination of Carbamazepine in Pure and Dosage Forms

Influence of the dual combination of silymarin and (-)-epigallocatechin gallate, natural dietary flavonoids, on the pharmacokinetics of oxcarbazepine in rats

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