HPLC Determination and Validation of Tramadol Hydrochloride in Capsules

Kartinasari, Wiwin Farina; Palupi, Tini; Indrayanto, Gunawan

doi:10.1081/jlc-120028261

Cited by 20 publications

(12 citation statements)

References 10 publications

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“…A number of assays have been reported for detection of TR in biological and pharmaceutical samples, including chromatographic [5][6][7][8][9][10][11] , mass spectrometric [11][12][13] , spectrophotometric [14][15][16] ,and Capillary electrophoresis 17 . However, chromatography requires the use of organic solvents, expensive instruments, and time-consuming operating steps [5][6][7][8][9][10] . The reported method of spectrophotometry was based upon the oxidation reaction between the drug and alkaline potassium permanganate,or was based on the reaction of tramadol hydrochloride with 4-chloro-7-nitrobenzofurazan (NBD-Cl) 13 .…”

Section: Introductionmentioning

confidence: 99%

Investigated the Supramolecular Interaction of Tramadol Hydrochloride With P-Sulfonated Calix[4,6,8]arene

Zhao

Chen

Pang

et al. 2015

J. Chil. Chem. Soc.

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In this paper, the interaction behavior of p-sulfonated calix[n]arene (SCnA) with tramadol hydrochloride was investigated by fluorescence spectra, 1 H NMR spectra, and theoretical calculations in aqueous solution. At the optimized conditions, the fluorescence intensity of tramadol hydrochloride showed positive correlation to the concentration of SCnA, which led to a validated, simple, and sensitive fluorescence quenching method for the determination of tramadol hydrochloride was established for the first time. Moreover, the interaction based on SCnA superstructure provided has promising potential for therapeutic monitoring and pharmacokinetics and clinical application

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Section: Introductionmentioning

confidence: 99%

Investigated the Supramolecular Interaction of Tramadol Hydrochloride With P-Sulfonated Calix[4,6,8]arene

Zhao

Chen

Pang

et al. 2015

J. Chil. Chem. Soc.

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“…TMH is official in European Pharmacopeia (EP) [4] which describe nonaqueous titration with perchloric acid as titrant the end point being located potentiometrically. Ultraviolet spectrophotometry [5,6], high-performance liquid chromatography [6][7][8][9], thin layer chromatography-densitometry [10], capillary isotachophoresis [11], flow injection chemiluminescence spectrometry [12], voltametry [13][14][15], ion-selective-based potentiometry [16][17][18][19][20][21][22], visible spectrophotometry [23][24][25][26][27][28], and titrimetry [26,29] for determining TMH in pharmaceutical dosage forms.…”

Section: Introductionmentioning

confidence: 99%

“…Simultaneous screening and quantification of 29 drugs of abuse including TMH in oral fluid [44] has been reported by Badawi et al Solid phase extraction and UPLC-MS/MS by Liquid chromatography-positive electrospray ionization tandem mass spectrometry has recently [45] been used for the multiresidue analysis of drugs of abuse in waste water and surface water. Though a number of liquid chromatographic methods have earlier proposed for TMH [5][6][7][8][29][30][31] no attempt has been made to apply this new type of LC (UPLC) for pharmaceuticals except in body fluids, oral fluids, and surface and waste waters [42][43][44][45] despite its many fold advantages.…”

Section: Introductionmentioning

confidence: 99%

A Stability Indicating UPLC Method for the Determination of Tramadol Hydrochloride: Application to Pharmaceutical Analysis

Vinay

Revanasiddappa

Xavier

et al. 2012

Chromatography Research International

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The use of Ultra Performance Liquid Chromatography (UPLC), with a rapid 5-minute reversed phase isocratic separation on a 1.7 µm reversed-phase packing material to provide rapid "high throughput" support for tramadol hydrochloride (TMH) is demonstrated. A simple, precise and accurate stability-indicating isocratic UPLC method was developed for the determination of TMH in bulk drug and in its tablets. The method was developed using Waters Aquity BEH C18 column (100 mm × 2.1 mm, 1.7 µm) with mobile phase consisting of a mixture of potassium dihydrogen phosphate buffer of pH 2.8 and an equal volume of acetonitrile (60 : 40 v/v). The eluted compound was detected at 226 nm with a UV detector. The standard curve of mean peak area versus concentration showed an excellent linearity over a concentration range 0.5-300 µg mL −1 TMH with regression coefficient (r) value of 0.9999. The limit of detection (S/N = 3) was 0.08 µg mL −1 and the limit of quantification (S/N = 10) was 0.2 µg mL −1 . Forced degradation of the bulk sample was conducted an accordance with the ICH guidelines. Acidic, basic, hydrolytic, oxidative, thermal and photolytic degradation were used to assess the stability indicating power of the method. TMH was found to degrade significantly in acidic, basic and oxidative stress conditions and stable in thermal, hydrolytic and photolytic conditions.

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“…A review of the literature revealed that several methods have been reported for the analysis of TMDH in pharmaceuticals. These methods include ultra‐violet spectrophotometry,4, 5 high performance liquid chromatography (HPLC),5–8 thin layer chromatrography‐densitometry,9 capillary isotachophoresis,10 flow injection chemiluminescence spectrometry,11 voltammetry,12–14 and ion‐selective‐based potentiometry 15–21…”

Section: Introductionmentioning

confidence: 99%

Use of two sulfonthalein dyes in the extraction‐free spectrophotometric assay of tramadol in dosage forms and in spiked human urine based on ion‐pair reaction

Vinay

Revannasiddappa

Rajendraprasad

et al. 2011

Drug Testing and Analysis

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Tramadol is a centrally acting analgesic used in the prevention and treatment of moderate to severe pain. Two sensitive, selective, and rapid spectrophotometric methods are described for the determination of tramadol in its dosage forms and in spiked human urine. The methods are based on formation of yellow ion-pairs between tramadol and two sulfonthalein dyes; bromocresol purple (BCP) and bromocresol green (BCG) in dichloromethane medium followed by absorbance measurement at 400 and 410 nm, respectively. Under the optimum conditions, tramadol could be assayed in the concentration ranges, 1-15 and 1-16 µg ml(-1) with correlation coefficient greater than 0.999 in both cases. The molar absorptivity values are calculated to be 1.84 × 10(4) and 1.97 × 10(4) l mol(-1) cm(-1) for BCP and BCG methods, respectively; and the corresponding Sandell sensitivity values are 0.0143 and 0.0134 µg cm(-2). The limits of detection (LOD) and quantification (LOQ) have also been reported. The stoichiometry of the reaction was found to be 1:1 in both cases and the conditional stability constant (K(f)) values of the ion pairs have been calculated. The within-day and between-day RSD were 0.9-1.96% and 1.56-3.21%, respectively. The methods were successfully applied to the determination of tramadol in tablets and injections and also in spiked human urine with good recoveries. The procedures are simple, accurate, and suitable for quality control application.

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HPLC Determination and Validation of Tramadol Hydrochloride in Capsules

Cited by 20 publications

References 10 publications

Investigated the Supramolecular Interaction of Tramadol Hydrochloride With P-Sulfonated Calix[4,6,8]arene

Investigated the Supramolecular Interaction of Tramadol Hydrochloride With P-Sulfonated Calix[4,6,8]arene

A Stability Indicating UPLC Method for the Determination of Tramadol Hydrochloride: Application to Pharmaceutical Analysis

Use of two sulfonthalein dyes in the extraction‐free spectrophotometric assay of tramadol in dosage forms and in spiked human urine based on ion‐pair reaction

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