HPLC determination of doxorubicin, doxorubicinol and four aglycone metabolites in plasma of AIDS patients

Beijnen, Jos H.; Meenhorst, Pieter L.; Gijn, R. van; Fromme, M.; Rosing, Hilde; Underberg, W.J.M.

doi:10.1016/0731-7085(91)80036-9

Cited by 38 publications

(22 citation statements)

References 13 publications

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“…Two major strategies have been followed: (1) an extraction followed by an evaporation step [13,42,61,63] or (2) an extraction followed by a back-extraction [27,53,68]. Since the first step is in both cases similar, this will be discussed together.…”

Section: Liquid-liquid Extractionmentioning

confidence: 99%

“…Isocratic water-acetonitrile mobile phases, containing a diluted acid [27,51,53,55,58,61,63] or an acidic buffer [52,56,60,64,65,67,68,72], sometimes in the presence of an additional organic modifier such as tetrahydrofuran [55,61,63,65] or triethylamine [27,51,56,58,64,72] have been employed frequently. Other isocratic mobile phases have been used occasionally [24,[44][45][46]62].…”

Section: Solid Phase Extractionmentioning

confidence: 99%

“…From the 35 publications included in this overview, 12 quantify only one main compound [26,[42][43][44][45][46][47][48][49][50][51][52]. Others determine a main compound and its reduced metabolite [27,[53][54][55][56][57][58][59], sometimes together with additional 8 metabolites [24,[60][61][62][63][64][65][66][67]. One method could be applied to each of the four pairs (main compound and respective reduced metabolite) individually [68].…”

Section: Analytes and Concentrations Of Interestmentioning

confidence: 99%

“…Ethylacetate [53], dichloromethane [13], and mixtures of chloroform with n-propanol [61,63] isopropanol [27,42] or 1-heptanol [68] have been used as extractants, mostly after addition of a mild alkaline buffer (buffer pH-range 8.5 to 9.5) to obtain high recoveries.…”

Section: Extraction Into An Organic Solvent (Mixture)mentioning

confidence: 99%

“…As is common in SPE, the choice of the stringency of the washing solvent is a compromise; given the same retention of the main compound, more stringent washing steps may lead to cleaner extracts, but with less retention of several metabolites. Reversed-phase C18 stationary phases have been widely used [13,24,26,27,44,45,50,51,55,56,58,60,62,64,65,67,72], but also C8 [46,52,61,63], cyano [66,68], phenyl [47] and phenyl-hexyl [53] stationary phases have been chosen.…”

Section: Solid Phase Extractionmentioning

confidence: 99%

See 4 more Smart Citations

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Maudens

Stove

Lambert

2011

Journal of Chromatography B

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Section: Liquid-liquid Extractionmentioning

confidence: 99%

Section: Solid Phase Extractionmentioning

confidence: 99%

Section: Analytes and Concentrations Of Interestmentioning

confidence: 99%

Section: Extraction Into An Organic Solvent (Mixture)mentioning

confidence: 99%

Section: Solid Phase Extractionmentioning

confidence: 99%

See 3 more Smart Citations

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Maudens

Stove

Lambert

2011

Journal of Chromatography B

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Tailoring Electrode Surface Charge to Achieve Discrimination and Quantification of Chemically Similar Small Molecules with Electrochemical Aptamers

Kesler

Chen

et al. 2022

Adv Funct Materials

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Electrochemical biosensors based on structure‐switching aptamers offer many advantages because they can operate directly in complex samples and offer the potential to integrate with miniaturized electronics. Unfortunately, these biosensors often suffer from cross‐reactivity problems when measuring a target in samples containing other chemically similar molecules, such as precursors or metabolites. While some progress has been made in selecting highly specific aptamers, the discovery of these reagents remains slow and costly. In this work, a novel strategy is demonstrated to distinguish molecules with miniscule difference in chemical composition (such as a single hydroxyl group)—with cross reactive aptamer probes—by tuning the charge state of the surface on which the aptamer probes are immobilized. As an exemplar, it is shown that the strategy can distinguish between DOX and many structurally similar analytes, including its primary metabolite doxorubicinol (DOXol). Then the ability to accurately quantify mixtures of these two molecules based on their differential response to sensors with different surface‐charge properties is demonstrated. It is believed that this methodology is general and can be extended to a broad range of applications.

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Therapeutic drug monitoring of doxorubicin in paediatric oncology using capillary electrophoresis

et al. 1998

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A method for the determination of doxorubicin and its main metabolite doxorubicinol in human plasma is described. Two different sample preparation procedures are applied depending on the expected concentration: To monitor the peak plasma levels, 10 microL of plasma are deproteinated with acetonitrile. After centrifugation, the supernatant is directly applied to the capillary by hydrodynamic injection. For the determination of lower amounts of doxorubicin and its main metabolite doxorubicinol 100 microL of plasma is extracted by liquid-/liquid extraction with chloroform. After evaporation of the organic phase, the sample is reconstituted in acetonitrile/water (95/5 v/v) and injected into the capillary by electrokinetic injection. Idarubicin serves as the internal standard. Laser-induced fluorescence detection with an Ar-ion laser emitting at 488 nm and a 520 nm cut-off filter is used for detection. The accuracy of the method was calculated to be 3.0% at higher concentrations and 15.0% at the limit of quantification. Reproducibility data are in accordance to the generally accepted criteria for bioanalytical methods. The limit of quantification is 2 microg/L, enabling us to monitor doxorubicin plasma levels for several days after application. Noninvasive blood sampling (from the fingertip) using heparinized capillaries was found to be a simple and convenient procedure and provides reproducible data. Initial results show high interindividual variability in doxorubicin peak plasma levels.

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HPLC determination of doxorubicin, doxorubicinol and four aglycone metabolites in plasma of AIDS patients

Cited by 38 publications

References 13 publications

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Tailoring Electrode Surface Charge to Achieve Discrimination and Quantification of Chemically Similar Small Molecules with Electrochemical Aptamers

Therapeutic drug monitoring of doxorubicin in paediatric oncology using capillary electrophoresis

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