HPLC Determination of Ochratoxin A in a Low Volume of Human Blood Serum

Ghali, R.; Hmaissia-khlifa, K.; Mezigh, Ch.; Ghorbel, H.; Maaroufi, Khira; Maschgoul, S.; Hedilli, Abderrazak

doi:10.1080/00032710801934528

Cited by 13 publications

(7 citation statements)

References 15 publications

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“…Currently, the most widely applied methods for OTA detection rely on chromatographic approaches (Tessini et al, 2010) and immunoassays (Meulenberg, 2012). Particularly, thin-layer chromatography (TLC) (Pittet and Royer, 2002), high performance liquid chromatography (HPLC) (Ghali et al, 2008) and enzyme-linked immunosorbent assay (ELISA) (Liu et al, 2008) are recommended by the Association of Official Agricultural Chemists and European Commission. But high cost, long processing times, and the need of specially trained operators have hindered chromatographic approaches' wide application.…”

Section: Introductionmentioning

confidence: 99%

Electrochemiluminescence biosensor for ultrasensitive determination of ochratoxin A in corn samples based on aptamer and hyperbranched rolling circle amplification

Yang

Zhang

et al. 2015

Biosensors and Bioelectronics

100

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Section: Introductionmentioning

confidence: 99%

Electrochemiluminescence biosensor for ultrasensitive determination of ochratoxin A in corn samples based on aptamer and hyperbranched rolling circle amplification

Yang

Zhang

et al. 2015

Biosensors and Bioelectronics

100

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“…The methods reported for the analysis of OTA are mainly chromatographic techniques including thin-layer chromatography (TLC), gas chromatography, and high-pressure liquid chromatography (HPLC). [24][25][26][27][28] Though chromatographic methods are very sensitive, they generally require multiple steps prior to detection, including extraction, extensive sample cleanup, pre-concentration, and sometimes derivatization of the analyte. To overcome the limitations encountered with chromatographic analysis, immunoassay methods have been developed.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-functionalized magnetic nanoparticle-based bioassay for the detection of ochratoxin a using upconversion nanoparticles as labels

Duan

Wang

et al. 2011

Analyst

133

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A sensitive luminescent bioassay for the detection of ochratoxin A (OTA), a small molecular mycotoxin, was developed using aptamer-conjugated magnetic nanoparticles (MNPs) as the recognition and concentration element and upconversion nanoparticles (UCNPs) as highly sensitive labels. The bioassay system was fabricated by immobilizing aptamer DNA 1 sequence onto the surface of Fe(3)O(4) MNPs, which were implemented to capture and concentrate OTA from bulk samples. The aptamer DNA 1 sequence then hybridized with UCNPs modified with DNA 2 sequence, which could dissociate from DNA 1 and result in a decreased luminescent signal when aptamer DNA 1 recognized and bound to target OTA. Under the optimal conditions, the decreased luminescent intensity (ΔI) is proportional to the concentration of OTA in the range of 1 × 10(-13) to 1 × 10(-9) g mL(-1) with a detection limit of 1 × 10(-13) g mL(-1). The proposed method then was successfully applied to measure OTA in naturally contaminated maize samples and validated by a commercially available enzyme-linked immunosorbent assay (ELISA) method. Benefiting from the magnetic separation and concentration effect of MNPs, the high sensitivity of UCNPs, as well as the selectivity and stability of the aptamer, the present upconversion luminescent bioassay offers a promising approach for the screening of small molecular mycotoxins because it is simple, rapid, highly sensitive, specific, does not require sample pre-concentration and lacks interference from autofluorescence of other biomolecules.

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“…Serum OTA was extracted by solid-phase extraction (SPE) based on Ghali et al [ 47 ] with some modifications. 0.5 mL of serum sample was acidified with 15 μL of acetic acid.…”

Section: Methodsmentioning

confidence: 99%

Maternal-Fetal Cancer Risk Assessment of Ochratoxin A during Pregnancy

Woo

El-Nezami

2016

Toxins

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Increasing evidence has demonstrated that in utero exposure to environmental chemicals may interfere with fetal development and increase the risk of disease and cancer development later in life. Ochratoxin A (OTA) has been proven to induce diverse toxic effects including teratogenicity, carcinogenicity, immunotoxicity and potential endocrine disruption. Due to the continuous and widespread occurrence of OTA as a potential contaminant of staple foods, there is increasing concern of in utero exposure of fetus to this mycotoxin. In this study, maternal-fetal risk assessment of OTA during pregnancy was conducted using the benchmark dose approach for genotoxic carcinogens. The daily intake of OTA for Egyptian pregnant women was estimated based on their serum OTA level using the refined Klaassen equation for pregnancy. Fetal exposure level was also estimated based on the maternal data. Comparison between the estimated daily exposure and the negligible cancer risk intake (NCRI), and the calculation of margin of exposure (MOE) implicated that OTA exposure from dietary intake would be of low health concern for this general subpopulation of Egyptian women. This subpopulation of pregnant women was generally estimated not to be in high-risk for toxicity induced by OTA.

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HPLC Determination of Ochratoxin A in a Low Volume of Human Blood Serum

Cited by 13 publications

References 15 publications

Electrochemiluminescence biosensor for ultrasensitive determination of ochratoxin A in corn samples based on aptamer and hyperbranched rolling circle amplification

Electrochemiluminescence biosensor for ultrasensitive determination of ochratoxin A in corn samples based on aptamer and hyperbranched rolling circle amplification

Aptamer-functionalized magnetic nanoparticle-based bioassay for the detection of ochratoxin a using upconversion nanoparticles as labels

Maternal-Fetal Cancer Risk Assessment of Ochratoxin A during Pregnancy

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