HPLC determination of pefloxacin and ciprofloxacin in blood serum

Brkich, G.E.; Арзамасцев, А. П.; Kaz'mina, E. M.; Mikhalëv, A. V.

doi:10.1007/bf02509945

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…Spectrophotometry 4,5 and high performance liquid chromatography (HPLC) 6,7 have been used for determination of ciprofloxacin hydrochloride. HPLC combined with either ultraviolet or fluorescence and mass spectrometry (MS) detection have been widely studied and were commonly used to analyze quinolone residues in biological samples.…”

Section: Introductionmentioning

confidence: 99%

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A novel method to determine ciprofloxacin by enhanced electrochemiluminescence of Tb(bpy)23+–K2S2O8 system in aqueous solution

Liu

Chen

et al. 2012

Anal. Methods

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A simple and rapid electrochemiluminescence (ECL) method is developed by using Tb(bpy) 2 3+ -K 2 S 2 O 8 system for the determination of trace amounts of ciprofloxacin (CPFX). Conditions for ECL emission were optimized. Under optimized condition, the linear range of ECL intensity versus concentrations of CPFX is 6 Â 10 À8 to 1.0 Â 10 À6 mol dm À3 with a relative standard deviation (R.S.D.) of 3.8% (n ¼ 11) and the detection limit is 1.4 Â 10 À9 mol dm À3 for CPFX. The ECL emission spectra (the peaks are at 490, 545, 585 and 620 nm) match the characteristic emission spectrum of Tb 3+ , indicating that the emission is from the excited state of Tb 3+ . The enhanced mechanism of Tb(bpy) 2 3+ -K 2 S 2 O 8 ECL system was proposed. This method was successfully applied to determine CPFX in tablets.

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Section: Introductionmentioning

confidence: 99%

“…(a) Tb(bpy)(bpy*)(NO 3 ) 3 (H 2 O) 3 -CPFX / Tb*(bpy) 2 (NO 3 ) 3 (H 2 O) 3 -CPFX (4) (b) Tb(bpy)(bpy*)(NO 3 ) 3 (H 2 O) 3 -CPFX / Tb(bpy) 2 (NO 3 ) 3 (H 2 O) 3 -CPFX* (5) Tb(bpy) 2 (NO 3 ) 3 (H 2 O) 3 -CPFX* / Tb*(bpy) 2 (NO 3 ) 3 (H 2 O) 3 -CPFX(6)Tb*(bpy) 2 (NO 3 ) 3 (H 2 O) 3 -CPFX / Tb(bpy) 2 (NO 3 ) 3 (H 2 O) 3 -CPFX + hn(7) …”

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confidence: 99%

A novel method to determine ciprofloxacin by enhanced electrochemiluminescence of Tb(bpy)23+–K2S2O8 system in aqueous solution

Liu

Chen

et al. 2012

Anal. Methods

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“…In order to proceed with this, it is necessary first to develop a reliable method for determining the drug in solution. According to published data [2], the most acceptable results can be obtained using HPLC. Indeed, this method in both reversed-and direct-phase variants has been successfully used for the quantitative determination of drugs belonging to the fluoroquinolone group.…”

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confidence: 99%

Optimized HPLC technique for determining ciprofloxacin hydrochloride in infusion solutions

et al. 2004

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Ciprofloxacin is a modern effective antibacterial drug widely used for the treatment of infectious disorders. The drug is available in various forms, including tablets, drops, capsules, and solutions. In serious cases, ciprofloxacin is administered in the form of dosed infusion preparations containing 200 mg of the parent drug in 100 ml of isotonic solution [1]. Foreign manufacturers prepare the infusions using ciprofloxacin base with lactic acid added as stabilizer. In some cases (ciprova, ciprofloxacin infusion solution from Lupin Laboratories Ltd., India), the preparation is additionally stabilized by adding sodium-EDTA salt.Russian manufacturers do not produce ciprofloxacin preparations in the form of solutions for infusion. Therefore, investigations in this direction are currently important. Since the State Register of Drugs includes only the parent compound of ciprofloxacin, it would be expedient to develop an infusion solution based on this substance. In order to proceed with this, it is necessary first to develop a reliable method for determining the drug in solution. According to published data [2], the most acceptable results can be obtained using HPLC. Indeed, this method in both reversed-and direct-phase variants has been successfully used for the quantitative determination of drugs belonging to the fluoroquinolone group. EXPERIMENTAL PARTBased on the results of preliminary experiments, we selected the following optimum composition of the infusion solution, which ensured required stability of the preparation: Ciprofloxacin hydrochloride 0.234 g Sodium chloride 0.9 g Citric acid 0.06 g Sodium hydrophosphate 0.1 g Water for injections to 100 mlThe HPLC procedure for the quantitative determination of ciprofloxacin hydrochloride in infusion solutions was developed using a liquid chromatograph of the Milikhrom-5 type equipped with a 2´80 mm column filled with Diasorb S-16 and a spectrophotometric detector tuned to 278 nm.According to published data [2 -4] on the HPLC analysis of fluoroquinolones, it is expedient to perform elution using solvent mixtures with acid components. In particular, Brkich [4] used a system with acetic acid. This was accompanied by the appearance of additional peaks in the chromatograms, which were explained by the peculiarities of ciprofloxacin adsorption on the HPLC column. However, we believe that the phenomenon observed in [4] was related to the existence of two forms of ciprofloxacin with different degrees of dissociation in solution. In such cases, it is expedient to use stronger acids such as phosphoric, which can provide for the complete protonation of nitrogen in the molecule of ciprofloxacin hydrochloride and for the suppression of ionization of the carboxy group.According to the U.S. Pharmacopoeia (USP-24), fluoroquinolones have to be eluted with a mixture of 0.025 M aqueous orthophosphoric acid solution (pH 3.0) and acetonitrile in a 87 : 13 ratio. We have used this solvent system and found that the peak of ciprofloxacin was asymmetric (the asymmetry coefficient calculat...

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Determination of Ciprofloxacin by Flow Injection Analysis Based on Chemiluminescence System

Zhang¹,

Wang

et al. 2006

Journal of Rare Earths

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HPLC determination of pefloxacin and ciprofloxacin in blood serum

Cited by 4 publications

References 9 publications

A novel method to determine ciprofloxacin by enhanced electrochemiluminescence of Tb(bpy)23+–K2S2O8 system in aqueous solution

A novel method to determine ciprofloxacin by enhanced electrochemiluminescence of Tb(bpy)23+–K2S2O8 system in aqueous solution

Optimized HPLC technique for determining ciprofloxacin hydrochloride in infusion solutions

Determination of Ciprofloxacin by Flow Injection Analysis Based on Chemiluminescence System

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