HPLC-ESI-MSn characterization of phenolic compounds, terpenoid saponins, and other minor compounds in Bituminaria bituminosa

Abstract

Tandem mass spectrometry a b s t r a c t Bituminaria bituminosa is a wild legume that can endure drastic conditions, including contaminated and degraded soils. It has been traditionally used as feeding for livestock, and different uses in folk medicine are known. The chemical composition of leaves and flowers from B. bituminosa is presented for the first time. The screening of phytochemical compounds was carried out using high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MS n ). More than 40 compounds were identified or tentatively characterized. A high percentage of the detected compounds corresponded to glycosylated flavonoids, especially from apigenin, although phenolic acids, lignans, and saponins were also identified.

LC‐PDA‐ESI‐MSn analysis of phenolic and iridoid compounds from Globularia spp.

“…These compounds showed a similar MS/MS fragmentation pattern with major fragments at m/z 229, 211 and 183 in MS 2 , MS 3 and MS 4 , respectively. They were tentatively identified in comparison to literature data as oxylipins, fatty acid oxidation products: oxo‐dihydroxy‐octadecenoic acid and trihydroxy‐octadecenoic acid …”

Traditionally Used Lathyrus Species: Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties, Cytotoxic Effects, and in silico Studies of L. czeczottianus and L. nissolia

“…Nine apigenin derivatives were tentatively characterized, most of them C-glycosides. Compound 12 was identified as vicenin-2 (apigenin-6,8-di-C-hexoside) considering bibliographic data (Llorent-Martínez et al, 2015 ). With a similar fragmentation pattern, compound 26 was also characterized as an apigenin-di-C-hexoside.…”

Physicochemical Characterization, Antioxidant Activity and Fingerprints of Industrialized “Detox” Mixed Beverages by Paper Spray Mass Spectrometry

“…Another compound that deserves prominence, identified as rosmarinic acid (m/z 359) that was found in samples A, D and F, presents anti-inflammatory action and has anti-pathogenic properties against bacterial and viral agents and neuroprotective effects. 37,38,43 According to El-Zaeddi et al 45 rosmarinic acid has already been reported in aromatic herbs. The samples A and B have the presence of parsley (Petroselinum crispum) an aromatic herb as a common ingredient among the samples.…”