HPLC Fingerprint of a Flower Extract of Tilia × viridis and Correlation with Antiproliferative and Antioxidant Activity

Abstract

Tilia species have been used in Europe and in America to treat anxiety and also for the treatment of colds, influenza, bronchitis, fever and inflammation. Tilia × viridis is a Tilia species distributed widely in Buenos Aires, Argentina. The flavonoids present in Tilia species have antioxidant properties, acting as reactive oxygen species (ROS) scavengers, principally on hydrogen peroxide (H(2)O(2)) and the superoxide anion (O(2)(·-)), which are involved in many diseases, including cancer. The aim of this study was to determine the phytochemical pattern of the ethanol extract of Tilia × viridis, principally the flavonoid content, and to evaluate the antiproliferative effects on both normal and tumoral cells, and the antioxidant activity in relation to H(2)O(2) modulation. The extract was found to present a selective antiproliferative activity on a lymphoma cell line and this was related to free radical scavenging activity. In addition, one of its main compounds, rutin, showed antioxidant effects related to peroxidase activity. T. × viridis may therefore be a source of antioxidant compounds that contribute to a selective antiproliferative action on tumoral cells, acting by modulation of H(2)O(2) levels.

Ethnopharmacological in vitro studies on Austria's folk medicine—An unexplored lore in vitro anti-inflammatory activities of 71 Austrian traditional herbal drugs

“…was detected in the present study. The flavonoids contained in the flowers of Tilia × viridis have been shown to exert antioxidant properties, acting as ROS scavengers, principally on hydrogen peroxide and the superoxide anion (Marrassini et al, 2011). Tilia argentea leaves and its two main flavonoid glycosides, kaempferol-3,7- O -α-dirhamnoside and quercetin-3,7- O -α-dirhamnoside, were evaluated for an antinociceptive activity, using the p -benzoquinone-induced writhing test and for an anti-inflammatory activity the carrageenan-induced hind paw edema model in mice.…”

Experimental studies on bioactive potential of rutin

“…[26] A number of plant extracts rich in flavonoids are known possess antimicrobial, anthelmintic, and cytotoxic potential. [27][28][29][30][31][32][33][34][35][36] The diverse therapeutic potential of plant flavonoids is due to their chemical structures. Such phytochemicals from plants serve as important therapeutic agents.…”

Linden – a rich source of phenolic compounds for health benefits

“…ROS przyczyniają się do rozwoju chorób nowotworowych i zwyrodnieniowych. Argentyńscy naukowcy analizowali etanolowe ekstrakty lipy z gatunku Tilia Virdis pod kątem aktywności antyproliferacyjnej, zarówno w limfocytach nowotworowych, jak i w limfocytach prawidłowo stymulowanych mitogenem [20]. Uzyskane wyniki wskazały na wzrost inhibicji proliferacji komórek w zależności od wzrostu stężenia ekstraktów lipy.…”