HPLC fingerprinting and LC–TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root

Han, Chao; Shen, Yan; Chen, Junhui; Lee, Frank Sen-Chun; Wang, Xiaoru

doi:10.1016/j.jchromb.2007.11.041

Cited by 42 publications

(20 citation statements)

References 14 publications

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“…The main objective of metabolic fingerprinting is to compare multiparametric patterns (or fingerprints) as dynamic metabolic phenotypes of a high number of samples (Wolfender et al 2009). This approach is widely applied in phytochemistry and phytotherapy for purposes such as quality control of plants, characterization and classification of medicinal plants (Fu et al 2009;Ge et al 2008;Han et al 2008;Yang et al 2007) and studies of plant response to stress and plant-host interactions (Allwood et al 2008;Shulaev et al 2008). Metabolomics approaches prove useful to distinguish among individual signals and thus might serve in biomarker discovery, for the identification of new chemotaxonomic characters in systematics, or as indicators of short-term environmental changes in ecology studies (Fiehn 2002;Wolfender et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Metabolic fingerprinting as an indicator of biodiversity: towards understanding inter-specific relationships among Homoscleromorpha sponges

et al. 2010

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Sponges are an important source of secondary metabolites showing a great diversity of structures and biological activities. Secondary metabolites can display specificity on different taxonomic levels, from species to phylum, which can make them good taxonomic biomarkers. However, the knowledge available on the metabolome of non-model organisms is often poor. In this study, we demonstrate that sponge chemical diversity may be useful for fundamental issues in systematics or evolutionary biology, by using metabolic fingerprints as indicators of metabolomic diversity in order to assess interspecific relationships. The sponge clade Homoscleromorpha is particularly challenging because its chemistry has been little studied and its phylogeny is still debated. Identification at species level is often troublesome, especially for the highly diversified Oscarella genus which lacks the fundamental characters of sponge taxonomy. An HPLC-DAD-ELSD-MS metabolic fingerprinting approach was developed and applied to 10 Mediterranean Homoscleromorpha species as a rapid assessment of their chemical diversity. A first validation of our approach was to measure intraspecific variability, which was found significantly lower than interspecific variability obtained between two Oscarella sister-species. Interspecific relationships among Homoscleromorpha species were then inferred from the alignment of their metabolic fingerprints. The resulting classification is congruent with phylogenetic trees obtained for a DNA marker (mitochondrial COI) and demonstrates the existence of two distinct groups within Homoscleromorpha. Metabolic fingerprinting proves a useful complementary tool in sponge systematics. Our case study calls for a revision of Homoscleromorpha with further phylogenetic studies and identification of additional chemical synapomorphic characters.

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Section: Introductionmentioning

confidence: 99%

Metabolic fingerprinting as an indicator of biodiversity: towards understanding inter-specific relationships among Homoscleromorpha sponges

et al. 2010

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“…This way the remaining samples constituted sets which have robust class memberships. However, the most popular clustering technique applied on herbal fingerprints is Hierarchical Clustering Analysis (HCA) displaying several different applications [44,101,122,[206][207][208][209][210][211]. In Reference [210], HCA was applied to identify the flavones, flavonoids and triterpenoids significantly contributing to the classification of six badges of sea buckthorn berries extracts, while in reference [211] the authors questioned the validity of the available monographs for the identification and differentiation of rhizoma Chuanxiong and rhizoma Ligustici based on the results of, amongst others, HCA.…”

Section: Clusteringmentioning

confidence: 99%

Chromatographic separation techniques and data handling methods for herbal fingerprints: A review

Tistaert

Dejaegher

Heyden

2011

Analytica Chimica Acta

324

275

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“…Afterwards, the extract was evaporated to dryness and then dissolved in 5 ml methanol. Samples were analyzed using an Agilent HPLC-MS system (Agilent 1200 surveyor autosampling system; Agilent 6300 ion trap mass spectrometer via an electrospray ion source, USA) according to Han et al (2008), with some modifications. Source settings used for the ionization of constituents were as follows: nebulizer pressure, 30.00 psi; drying gas, 12.0 l min −1 ; capillary voltage, 3500 V; gas temperature, 350°C; fragmentor voltage, 200 V; and skimmer voltage, 60 V. Nitrogen (>99.99 %) and He (>99.99 %) were used as sheath and damping gases, respectively.…”

Section: Hplc-ms and Esi-ms Nmentioning

confidence: 99%

“…[M+CH 3 COO] − is 874.3, so peak 4 was deduced as pseudostellarin G. The standardized HPLC-ESI-MS n shows high stability and reproducibility and thus can be used effectively for the screening analysis or quality assessment of the herb or its derived products [15]. Based on these reasons, a reliable HPLC-ESI-MS n method for the identification and quality evaluation of the P. heterophylla adventitious roots was established.…”

Section: Lc-ms N Analysis Of Adventitious Rootsmentioning

confidence: 99%

HPLC–ESI–MSn Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla

Wang

et al. 2015

Appl Biochem Biotechnol

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A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots. Pseudostellarin A, C, D, and G were identified from P. heterophylla adventitious roots on the basis of LC-MS(n) analysis. The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %. Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR). The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group. PLS-DA showed that polysaccharides contributed significantly to the clustering of different groups and showed a positive correlation in the MS medium group. The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla. As a result, AR possessed a similar immunoregulation function as NR.

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HPLC fingerprinting and LC–TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root

Cited by 42 publications

References 14 publications

Metabolic fingerprinting as an indicator of biodiversity: towards understanding inter-specific relationships among Homoscleromorpha sponges

Metabolic fingerprinting as an indicator of biodiversity: towards understanding inter-specific relationships among Homoscleromorpha sponges

Chromatographic separation techniques and data handling methods for herbal fingerprints: A review

HPLC–ESI–MSn Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla

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