HPLC fractionation and pharmacological assessment of green tea seed saponins for antimicrobial, anti-angiogenic and hemolytic activities

Kim, Jong Deog; Khan, Muhammad Imran; Shin, Jin Hyuk; Lee, Moon Geon; Seo, Hyo Jin; Shin, Tai Sun; Kim, Min Yong

doi:10.1007/s12257-015-0538-6

Cited by 23 publications

(11 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strains used were Escherichia coli (ATCC 25922), Salmonella typhimurium (ATCC 14028), Salmonella enteritidis (ATCC 13076), Salmonella gallinarum (ATCC 9184), Salmonella choleraesuis (ATCC 7001), Salmonella pullorum (ATCC 19945), Salmonella dublin (ATCC 15480), and Staphylococcus aureus (ATCC 12600). Disc-diffusion method was used as a preliminary test and then the antibacterial activities were also determined by bacteria viability and lethality in the pressence of verious concentrations of saponin fractions [21]. The inoculum of bacteria was prepared in Luria–Bertani broth (Difco, USA) and nurturant broth (Difco) (2 different growth media were tested individually for best growth and inoculum preparation) and then 500 μ L active culture of each strain was spread on the respective Muller-Hinton agar (BD biosciences) plates.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Green Tea Seed Isolated Saponins Exerts Antibacterial Effects against Various Strains of Gram Positive and Gram Negative Bacteria, a Comprehensive Study In Vitro and In Vivo

Khan

Ahhmed

Shin

et al. 2018

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

Bacteria are one of the major causes of severe infections and diseases of plants and animals. Salmonella are crucially important due to infection in poultry leading to huge economical loses. Due to high cost and microbial resistance to the currently available chemical antibiotics, demand of screening natural products with antibiotics effects is increased. Plants are rich sources of natural bioactive compounds with antibiotic effects. Saponins are natural compounds of plant sources having a diverse range of applications. In present study we investigated the in vitro and in vivo antibacterial activities of green tea seed extracted saponins. Green tea seeds crude extract was prepared in 70% ethanol by continuous reflux in heating mantel for 5 hours. Crude saponins were extracted from the crude ethanolic extract of green tea seed by column chromatography using macroporous resin (D101). Saponin mixture in fraction 1 (Fr1) was obtained from crude saponins extract via column chromatography. Fr2 and Fr3 were isolated from saponins mixture by preparative HPLC. Antibacterial activities of the isolated saponins fractions were investigated against Escherichia coli (ATCC 25922), Streptococcus aureus (ATCC 12600), and six serovars of Salmonella. In vitro antibacterial activities were determined by disc-diffusion method and growth inhibition in liquid culture using 96-well plate. Results showed that the green tea isolated saponins fractions possess antibacterial effects in the following order Fr1>Fr2>Fr3. Antibacterial mechanism of saponins was elucidated by cell wall and membrane damaging potential of saponins determined by measuring AKP and soluble proteins levels. Fr1 was further used for in vivo antibacterial activities. Five-week grown chickens were selected for in vivo work, divided into three groups as control, infected, and treatment groups. Infected and treatment groups chickens were infected with bacteria and only treatment group chickens were treated with saponins. The qRT- PCR analysis of the blood and feces samples of the different groups' animals shows the presence of bacteria only in infected group while reduced expression levels of the bacterial pathogens were found in the samples of treatment group. Our results demonstrated that the green tea seed saponins used in this study possess strong antibacterial activities.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The minimum inhibitory concentrations (MIC) of saponins fractions were determined as previously described [21]. In brief bacteria were grown in their respective liquid culture and 20 μ L of active cultures of each organism was inoculated into 100 μ L nutrient broth (Difco) per well of the 96-well plate.…”

Section: Methodsmentioning

confidence: 99%

Green Tea Seed Isolated Saponins Exerts Antibacterial Effects against Various Strains of Gram Positive and Gram Negative Bacteria, a Comprehensive Study In Vitro and In Vivo

Khan

Ahhmed

Shin

et al. 2018

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Green tea possess a number of bioactive compounds with profound medicinal and pharmacological values. Green tea has been reported for anticancer, antidiabetic, anti-obesity, antiangiogenic and antimicrobial activities [43][44][45][46] Green tea has therapeutic effects on human brain function, ameliorates liver injury and enhances immunity. [47,48].…”

Section: Discussionmentioning

confidence: 99%

“…A voucher number (HCCN60938) was obtained for the specimen. Pure saponins were extracted from green tea (Camellia sinensis (L.) O. Kuntze) seeds (GTS) as previously described with some improvement [43,44]. Briefly, GTS were ground into fine powder and defatted with n-hexane by sonication at 30 • C for 6 h and then dried.…”

Section: Extraction Of Saponinsmentioning

confidence: 99%

Green Tea Seed Isolated Theasaponin E1 Ameliorates AD Promoting Neurotoxic Pathogenesis by Attenuating Aβ Peptide Levels in SweAPP N2a Cells

et al. 2020

Self Cite

View full text Add to dashboard Cite

Alzheimer’s disease (AD) is the most frequent type of dementia affecting memory, thinking and behaviour. The major hallmark of the disease is pathological neurodegeneration due to abnormal aggregation of Amyloid beta (Aβ) peptides generated by β- and γ-secretases via amyloidogenic pathway. Purpose of the current study was to evaluate the effects of theasaponin E1 on the inhibition of Aβ producing β-, γ-secretases (BACE1, PS1 and NCT) and acetylcholinesterase and activation of the non-amyloidogenic APP processing α-secretase (ADAM10). Additionally, theasaponin E1 effects on Aβ degrading and clearing proteins neprilysin and insulin degrading enzyme (IDE). The effect of theasaponin E1 on these crucial enzymes was investigated by RT-PCR, ELISA, western blotting and fluorometric assays using mouse neuroblastoma cells (SweAPP N2a). theasaponin E1 was extracted and purified from green tea seed extract via HPLC, and N2a cells were treated with different concentrations for 24 h. Gene and protein expression in the cells were measured to determine the effects of activation and/or inhibition of theasaponin E1 on β- and γ-secretases, neprilysin and IDE. Results demonstrated that theasaponin E1 significantly reduced Aβ concentration by activation of the α-secretase and neprilysin. The activities of β- and γ-secretase were reduced in a dose-dependent manner due to downregulation of BACE1, presenilin, and nicastrin. Similarly, theasaponin E1 significantly reduced the activity of acetylcholinesterase. Overall, from the results it is concluded that green tea seed extracted saponin E1 possess therapeutic significance as a neuroprotective natural product recommended for the treatment of Alzheimer’s disease.

show abstract

“…So far, more than 50 saponin compounds have been isolated and identified from the seeds of C. sinensis cultivated in China, Japan, Sri Lanka, and India [8]. Pharmacological studies indicated that these saponins could protect gastric mucosa of rats induced by ethanol or indomethacin [9], inhibit gastric emptying and alcohol absorption in mice [10], suppress ascites cancer S180 cells, myelocytic leukemia K562 cells, promyelocytic leukemia HL-60 cells, cisplatin-resistant ovarian cancer OVCAR-3 and A2780/CP70 cells in vitro and in vivo [11,12], block inflammatory pathways composed of AKT, IKK, and NF-κB in macrophages stimulated by fluorescein isothiocyanate-dextran, sodium nitroprusside and lipopolysaccharide [13], and have a wide spectrum of anti-bacterial and anti-fungal activities [14,15,16]. In addition, C. sinensis seed saponins could promote uptake of Cd by Amaranthus caudatus , and were more efficient than ethylenediaminetetracetic acid (EDTA) [17].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination and Quantification of Triterpene Saponins from Camellia sinensis Seeds Using UPLC-PDA-QTOF-MS/MS

Jia

et al. 2019

Molecules

View full text Add to dashboard Cite

Saponins in the Camellia sinensis seeds have a broad spectrum of biological properties and application potentials. However, up to now, no chromatographic methods have been developed to provide full fingerprinting and quality assurance for these saponins. This research aimed to develop a novel method to tentatively identify and quantify saponins in C. sinensis seeds by ultra-high-performance liquid chromatography coupled with photo-diode array detector and quadrupole time-of-flight mass spectrometry (UPLC-PDA-QTOF-MS/MS), and compare it with the classic vanillin-sulfuric acid assay. Fifty-one triterpene saponins, including six potentially new compounds, were simultaneously detected by UPLC-PDA-MS/MS, and their chemical structures were speculated according to the retention behavior and fragmentation pattern. The total saponin content in the crude extract and the purified saponin fraction of C. sinensis seeds were quantified to be 19.57 ± 0.05% (wt %) and 41.68 ± 0.09% (wt %) respectively by UPLC-PDA at 210 nm, while the corresponding values were determined to be 43.11 ± 3.17% (wt %) and 56.60 ± 5.79% (wt %) respectively by the vanillin-sulfuric acid assay. The developed UPLC-PDA -MS/MS method could determine specified saponins, and is more reliable for quantifying the C. sinensis seed saponins than the classic spectrophotometric method. It is of great significance for the future investigations and applications of these saponins.

show abstract

HPLC fractionation and pharmacological assessment of green tea seed saponins for antimicrobial, anti-angiogenic and hemolytic activities

Cited by 23 publications

References 29 publications

Green Tea Seed Isolated Saponins Exerts Antibacterial Effects against Various Strains of Gram Positive and Gram Negative Bacteria, a Comprehensive Study In Vitro and In Vivo

Green Tea Seed Isolated Saponins Exerts Antibacterial Effects against Various Strains of Gram Positive and Gram Negative Bacteria, a Comprehensive Study In Vitro and In Vivo

Green Tea Seed Isolated Theasaponin E1 Ameliorates AD Promoting Neurotoxic Pathogenesis by Attenuating Aβ Peptide Levels in SweAPP N2a Cells

Simultaneous Determination and Quantification of Triterpene Saponins from Camellia sinensis Seeds Using UPLC-PDA-QTOF-MS/MS

Contact Info

Product

Resources

About