HPLC: Highly Accessible Instrument in Pharmaceutical Industry for Effective Method Development

Abstract

The analytical technique of High Performance Liquid Chromatography (HPLC) is used extensively thought the pharmaceutical industry. It allows simultaneously both qualitative and quantitative information of a drug. It is used to provide information on the composition of drug related samples. The information obtained may be qualitative, indicating what compounds are present in the sample. The information obtained may be qualitative, providing the actual amount of compounds in the sample. HPLC is used in all the different stages in the creation of new drug discoveries and also used routinely in the drug manufacturing process. The HPLC techniques is highly accessible to the author's knowledge this review represents the most exhaust description of instrumentation, method development, recent advancement, area of application. This article reviews the most recent advances in sample preparation, separation and the mass spectrometric aspects of high-throughput for method development.

A Review of Methods for Removal of Ceftriaxone from Wastewater

“…HPLC employs various types of the stationary phases, and the pump that drives the mobile phase(s) and analyte through the column and detector to provide a characteristic retention time for the analyte. The retention period of an analyte varies according to the strength of its interactions with the stationary phase, solvent(s) ratio/composition utilized, and flow rate of the mobile phase [ 47 ]. HPLC has a number of advantages, including low organic solvent utilization, minimal sample volume, quick analysis, and high chromatographic resolution [ 48 ].…”