HPLC–ICP-MS speciation of selenium in enriched onion leaves – a potential dietary source of Se-methylselenocysteine

Wróbel, Katarzyna; Wróbel, Kazimierz; Kannamkumarath, Sasi S.; Caruso, Joseph A.; Wysocka, Irena; Bulska, Ewa; Świa̧tek, Justyna; Wierzbicka, Małgorzata

doi:10.1016/j.foodchem.2003.11.005

Cited by 89 publications

(65 citation statements)

References 26 publications

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“…In both treatments, SeMet was the dominant organic Se species, probably because SeMet is randomly incorporated into proteins replacing methionine (Navarro-Alarcon and Cabrera-Vique, 2008). The observed Se speciation for both treatments is in good agreement with results reported for other food crops such as Se-enriched Allium species (Wróbela et al, 2004;Pedreroa et al, 2007). Even though organic Se compounds are less toxic, when the dominant organic Se form is SeMet, it can be directly incorporated into general proteins instead of other Se compounds which were most effective in reducing tumors.…”

Section: Discussionsupporting

confidence: 88%

Use of selenium fertilizers for production of Se‐enriched Kenaf (Hibiscus cannabinus): Effect on Se concentration and plant productivity

Lavu

Schepper

Steppe

et al. 2013

Z. Pflanzenernähr. Bodenk.

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Due to selenium (Se) deficiency, Se fortification of food and feed is applied in many countries. Therefore, potential use of Se-enriched kenaf was investigated based on its Se accumulation, its potential to transform accumulated Se to other Se species, and effect of Se accumulation on its growth. Kenaf was grown with different levels of two Se fertilizers (selenite and selenate) at concentrations ranging from 0 to 4 mg Se (kg soil) -1 . Total Se concentrations in the plants grown on selenate-treated soil amounted to (1019 ± 136) mg Se (kg dry weight) -1 and were much higher compared to plants grown on selenite-treated soil. Identified Se species were selenite, selenate, Se-methionine, and Se-cystine. Biomass yield, net photosynthesis, and chlorophyll index of the plants decreased when plants were grown on soils treated with high doses of selenate.

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Section: Discussionsupporting

confidence: 88%

Use of selenium fertilizers for production of Se‐enriched Kenaf (Hibiscus cannabinus): Effect on Se concentration and plant productivity

Lavu

Schepper

Steppe

et al. 2013

Z. Pflanzenernähr. Bodenk.

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“…Selenium is supplemented either in inorganic forms such as sodium selenite, sodium selenate, barium selenate, or, more recently, in organic forms such as selenomethionine and selenium yeast (which contain Se bound primarily as selenomethionine), selenium chelates (where selenium is part of a chelate complex with proteins or amino acids), Se-methylselenocysteine contained in Se-enriched plants such as onion, garlic, broccoli, cabbage or radish (Wrobel et al, 2004;Pedrero et al, 2006;Kapolna and Fodor, 2006;Yamanoshita et al, 2007). In human nutrition, also other foodstuffs are increasingly used for their anticarcinogenic and antioxidative effects.…”

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confidence: 99%

Effect of a long-term peroral supplementation with sodium selenite and selenium lactate-protein complex on selenium status in goats and their kids

Mišurová¹,

Pavlata²,

Pechová³

et al. 2009

Vet. Med.

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AbstrAct:The aim of this study was to evaluate the effect of a long-term peroral selenium supplementation in the form of sodium selenite and selenium lactate-protein complex by comparing selenium concentrations and glutathione peroxidase activity in blood of goats and their kids as well as comparing selenium concentrations in goat colostrums. For the study, a total of 27 clinically healthy pregnant white shorthair goats were used. They were divided to three groups, i.e., the control group (C) without any selenium supplementation, sodium selenite group (E1) and selenium lactate-protein complex group (E2). For four months, experimental goats received 0.43 mg of selenium per animal per day in diet; goats from the control group were given 0.15 mg of selenium per animal per day. At the beginning of the experiment, goats of all groups showed an average selenium concentration of 96 μg/l in whole blood. On the parturition day, samples of first colostrum from goats and heparinized blood from goats and kids were taken. In the control group (C), average blood selenium concentrations of 111.4 ± 33.5 μg/l were observed on the parturition day. In both experimental groups, selenium concentrations were significantly higher (P < 0.05). Average selenium concentration in the sodium selenite group (E1) was 177.2 ± 34.8 μg/l and in the group supplemented with selenium lactate-protein complex (E2) 159.0 ± 28.5 μg/l. Average glutathione peroxidase (GSH-Px) activity in blood of control goats (C) was 581.9 ± 99.2 μkat/l, in group E1 1 154.6 ± 156.2 μkat/l and in group E2 1 011.6 ± 153.6 μkat/l. GSHPx activity in experimental groups was significantly higher (P < 0.05) as compared with the control group. Average selenium concentrations in colostrum was in the control group 40.1 ± 12.8 μg/l, in E1 99.0 ± 29.9 μg/l and in group E2 79.0 ± 17.7 μg/l. Colostral selenium concentrations in experimental groups were significantly higher (P < 0.05) as compared with the control group. No significant difference in the monitored parameters was found between experimental groups. In kids of control mothers (kC), average selenium concentrations in blood on the parturition day were 62.4 ± 22.9 μg/l; kids of mothers supplemented with sodium selenite (kE1) showed average selenium levels of 100.0 ± 31.2 μg/l, and the average selenium concentration in kids of mothers receiving lactate-protein complex was 83.4 ± 20.1 μg/l (kE2). Average GSH-Px activity in control kids (kC) was 402.1 ± 153.9 μkat/l. Kids from kE1 showed average activity of GSH-Px 806.1 ± 254.9 μkat/l and kids from group kE2 529.9 ± 119.8 μkat/l. Statistically significant difference (P < 0.05) was found only between kC and kE1 which showed significantly higher selenium concentration and GSH-Px activity. The results of this study confirm that both forms of selenium administered in experimental groups (i.e., sodium selenite and selenium lactate-protein complex) had similar biological effect in goats. However, results obtained in kids indicate a better effect of supplementation with sodium selenite.

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“…9 For determination of water soluble species of selenium in biological samples, the most often used techniques are ion exchange (cation and anion) and reverse phase liquid chromatography with different detection systems (ICP-MS, AAS, AFS, ETAAS). 10 In the literature, HPLC is most often used in connection with ICP-MS, 4,[11][12][13][14][15] HG-AAS 16 and HG-AFS. 7,[17][18][19] For separation of volatile selenium species, GC may be used.…”

mentioning

confidence: 99%

Selenium Species Determination in Selenium-Enriched Pumpkin (Cucurbita pepo L.) Seeds by HPLC-UV-HG-AFS

et al. 2005

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In the last decade, many studies have been conducted to enhance the selenium content of edible plants by different types of cultivation. Although it is not known if Se exerts any beneficial effects on the plants themselves, Se enrichment of plants improves the nutritional status of selenium in man. Recently, some studies have shown that appropriate selenium addition to plants promotes their growth and is also supposed to increase their antioxidative capacity, helping to decrease oxidative stress in plants. 1,2 The reactivity and nutritional bioavailability of selenium in selenium-enriched plants depend not only on the total concentration, but predominantly on the selenium species present. Consequently, it is very important to determine the chemical species of selenium present in plants. 3 Selenium-enriched plants accumulate selenium in different forms: cereals and yeast predominantly as SeMet, and Allium plants as Se-methylselenocysteine (SeMeSeCys). [4][5][6] Bodo et al. determined the selenium species in Brazil nuts and, because of the high fat content of the samples, they defatted them first by extraction with cyclohexane; only 0.2% of selenium was lost in this procedure. 7 The main selenium species in these nuts was SeMet, representing 96% of the total Se content; traces of selenocystine (SeCys2) were also found. Another study on selenium speciation in some nuts (Brazil nuts, walnuts…) was made by Kannamkumarath et al. and much lower contents for Se in the form of SeMet were obtained (19 -25%). They used chloroform and methanol (2:1) to remove fat.8 All nuts were purchased on the market, so no data on the origin of samples were given.A renewed nutritional interest in pumpkins has arisen because of its high nutritional value; the pumpkin contains many nutritional sterols, vitamins B and E, and minerals such as Ca, K, P, Mg, Mn, Fe and Zn. Pumpkin seeds have been used for centuries in traditional medicine, mainly in the case of problems of the kidney or the urinary tract, and against tapeworms. They are normally only a moderate source of selenium. But lately several studies were made to enhance the selenium content in edible plants by different types of cultivation. In our previous work, we already studied the ability of pumpkin seeds to accumulate selenium by foliar application of an aqueous solution containing 1 mg Se/L and we found that the Se content of Se treated plants was about 3.5-fold higher than in nontreated ones. So it was concluded that pumpkin seeds are an appropriate food item for selenium enrichment. 9 For determination of water soluble species of selenium in biological samples, the most often used techniques are ion exchange (cation and anion) and reverse phase liquid chromatography with different detection systems (ICP-MS, AAS, AFS, ETAAS). 10 In the literature, HPLC is most often used in connection with ICP-MS, 4,11-15 HG-AAS 16 and HG-AFS. 7,[17][18][19] For separation of volatile selenium species, GC may be used. 20 The aim of this work was to identify and quantify the selenium species in ...

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HPLC–ICP-MS speciation of selenium in enriched onion leaves – a potential dietary source of Se-methylselenocysteine

Cited by 89 publications

References 26 publications

Use of selenium fertilizers for production of Se‐enriched Kenaf (Hibiscus cannabinus): Effect on Se concentration and plant productivity

Use of selenium fertilizers for production of Se‐enriched Kenaf (Hibiscus cannabinus): Effect on Se concentration and plant productivity

Effect of a long-term peroral supplementation with sodium selenite and selenium lactate-protein complex on selenium status in goats and their kids

Selenium Species Determination in Selenium-Enriched Pumpkin (Cucurbita pepo L.) Seeds by HPLC-UV-HG-AFS

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