HPLC identification of isoniazid residues in bovine milk

Abstract

The high pressure liquid chromatography (HPLC) was used for the identification of isoniazid (isonicotinic acid hydrazide) in the milk of cattle treated with a dose of 25 mg/kg/day in alternated days. The effect of milk pasteurization on the isoniazid residue concentration was also studied. The drug excretion presented a cyclic variation, with higher levels in the first day after administration (aa), a mean of 1104.48µg/l, and a decrease two days aa, with a mean of 104.12µg/l. Four days after the last administration of the drug it was not possible to identify residues of isoniazid in the milk of treated animals. Body weight and milk yield influenced the amount of the excreted drug, and pasteurization decreased (mean 47.07%) the concentration of isoniazid residue in milk.

Is Interleukin-4δ3 Splice Variant Expression in Bovine Tuberculosis a Marker of Protective Immunity?

“…The calves were divided into three groups of four animals. The first group received 350 CFU virulent M. bovis (AF2122/97) by the intratracheal route as described above plus isoniazid (INH) (25 mg/kg/day, as described by Leite et al [21]) from 3 weeks postinfection until 17 weeks postinfection. This group was then rested until the postmortem examination at 34 weeks postinfection.…”

Antituberculosis drug isoniazid degraded by electro-Fenton and photoelectro-Fenton processes using a boron-doped diamond anode and a carbon-PTFE air-diffusion cathode

“…Isoniazid (INH, C 6H7N3O, isonicotinic acid hydrazide) is a widely used antibiotic against tuberculosis, a disease that affects at least a third of the world's population (Scior et al 2002) as well bovine herds (Leite et al 2000). As occurs with many other drugs, up to 75% of the parent compound can be excreted unchanged from the body (Sasu et al 2015), being able to enter into water bodies.…”