HPLC method development, validation and its application to investigate <i>in vitro</i> effect of pioglitazone on the availability of H<sub>1</sub> receptor antagonists

Mirza, Agha Zeeshan; Arayne, M. Saeed; Sultana, Najma

doi:10.1016/j.jaubas.2015.12.001

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…Validation of the analytical method was carried out in accordance with international conference on harmonization (ICH) guidelines, for which the following criteria were analyzed: linearity, precision, accuracy, robustness, specificity, limits of detection, and quantification. The objective of validating an analytical method is to confirm that the analytical procedure employed for a specific test is suitable for its intended use [ 40 , 41 ]. Spectra showed maximum absorbance at a wavelength of 269 nm and the calibration curve was found to be linear in the concentration range of 1.5–110 μg/mL, with a correlation coefficient ( r 2 ) value of 0.0998 ( Figure S1 ).…”

Section: Discussionmentioning

confidence: 99%

Human Skin Permeation Studies with PPARγ Agonist to Improve Its Permeability and Efficacy in Inflammatory Processes

Silva-Abreu

Espinoza

Rodríguez-Lagunas

et al. 2017

IJMS

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Rosacea is the most common inflammatory skin disease. It is characterized by erythema, inflammatory papules and pustules, visible blood vessels, and telangiectasia. The current treatment has limitations and unsatisfactory results. Pioglitazone (PGZ) is an agonist of peroxisome proliferator-activated receptors (PPARs), a nuclear receptor that regulates important cellular functions, including inflammatory responses. The purpose of this study was to evaluate the permeation of PGZ with a selection of penetration enhancers and to analyze its effectiveness for treating rosacea. The high-performance liquid chromatography (HPLC) method was validated for the quantitative determination of PGZ. Ex vivo permeation experiments were realized in Franz diffusion cells using human skin, in which PGZ with different penetration enhancers were assayed. The results showed that the limonene was the most effective penetration enhancer that promotes the permeation of PGZ through the skin. The cytotoxicity studies and the Draize test detected cell viability and the absence of skin irritation, respectively. The determination of the skin color using a skin colorimetric probe and the results of histopathological studies confirmed the ability of PGZ-limonene to reduce erythema and vasodilation. This study suggests new pharmacological indications of PGZ and its possible application in the treatment of skin diseases, namely rosacea.

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Section: Discussionmentioning

confidence: 99%

Human Skin Permeation Studies with PPARγ Agonist to Improve Its Permeability and Efficacy in Inflammatory Processes

Silva-Abreu

Espinoza

Rodríguez-Lagunas

et al. 2017

IJMS

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Separation and Assay of Four Antihistamine Drugs Diphenhydramine, Chlorpheniramine, Cyproheptadine and Fexofenadine in Pharmaceutical Forms by a Single HPLC Method

Shasho

Sakur

Trefi

2018

Int J Pharm Pharm Sci

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Objective: The objective of this study was to develop and validate a single HPLC method, in order to separate and assay four antihistamine drugs diphenhydramine, chlorpheniramine, cyproheptadine and fexofenadine in pharmaceutical forms. This method was a practical additional choice in quality control laboratories.Methods: The chromatographic conditions comprised of a classical C18-type stationary phase (150 × 4.6 mm, 5μ), with a mobile phase consisting of, 2.5g of sodium octane sulfonic acid in a mixture of 500 ml of deionized water and 500 ml of acetonitrile, and apparent pH of 2.0 was adjusted with phosphoric acid. The flow rate was 1 ml/min; the detection wavelengths were at 220 nm, 230 nm, 265 nm and 254 nm for diphenhydramine, chlorpheniramine, cyproheptadine and fexofenadine respectively. The temperature was ambient temperature.Results: The method was validated for linearity with correlation coefficients very close to one, the accuracy with mean recovery values between 95.0-105.0%, precision with relative standard deviations of the calculated concentrations less than 5.0% and specificity in the presence of degradation products. Then it was used successfully to separate a mixture of them and to assay these drugs in pharmaceutical forms purchased from Syria.Conclusion: The results presented in this paper showed that the developed method was simple and applicable, for the separation and determination of the four drugs in their pharmaceutical forms.

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Oxidimetric determination of Cetirizine and Levocetirizine in tablets using Oxone

Deghinmotei,

Mykola Yevstahiyovich,

Ebena

et al. 2024

RJPT

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This work is aimed at studying the kinetics of Cetirizine (CTZ) and Levocetirizine(L-Cet) oxidation by potassium hydrogen peroxomonosulfate (PMS) in the form of Oxone and developing an analytical method for their quantitative determination. The required amount of CTZ and L-Cet was dissolved in double distilled water, pH buffer solution and PMS solution were added. After 1.5min, the solution was acidified. The excess PMS was iodometrically titrated applying visual end-point detection approach. The reaction was studied in aqueous media at 293 K and over pH range 7.0 to 10.0. The second order rate constants, k (L mol−1 min−1) has been calculated from kinetic data. A suitable scheme has been proposed for the oxidation of CTZ and L-Cet with PMS, which shows electrophilic attack of the monoanion of PMS on a nitrogen atom of the analyzed compounds with the formation of the corresponding amine oxides. CTZ and L-Cet were determined by indirect titration with PMS. The recovery of the assayed analytes in tablets ranged from 99.4 to 101.0%. A paired t-test showed that all results obtained for CTZ and L-Cet in model solutions and dosage form of Levocetirizine (5mg) and Сetirizine (10mg) tablets, using the proposed procedure and the official procedure respectively, were in consonance at the 95 % confidence level, (х ̅ -μ) 100/μ < RSD. Techniques have been developed and the possibility of quantitative determination of Levocetirizine and Cetirizine in substance and tablet forms using Oxone as an analytical reagent has been demonstrated.

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HPLC method development, validation and its application to investigate in vitro effect of pioglitazone on the availability of H₁ receptor antagonists

Cited by 4 publications

References 24 publications

Human Skin Permeation Studies with PPARγ Agonist to Improve Its Permeability and Efficacy in Inflammatory Processes

Human Skin Permeation Studies with PPARγ Agonist to Improve Its Permeability and Efficacy in Inflammatory Processes

Separation and Assay of Four Antihistamine Drugs Diphenhydramine, Chlorpheniramine, Cyproheptadine and Fexofenadine in Pharmaceutical Forms by a Single HPLC Method

Oxidimetric determination of Cetirizine and Levocetirizine in tablets using Oxone

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HPLC method development, validation and its application to investigate in vitro effect of pioglitazone on the availability of H1 receptor antagonists

Cited by 4 publications

References 24 publications

Human Skin Permeation Studies with PPARγ Agonist to Improve Its Permeability and Efficacy in Inflammatory Processes

Human Skin Permeation Studies with PPARγ Agonist to Improve Its Permeability and Efficacy in Inflammatory Processes

Separation and Assay of Four Antihistamine Drugs Diphenhydramine, Chlorpheniramine, Cyproheptadine and Fexofenadine in Pharmaceutical Forms by a Single HPLC Method

Oxidimetric determination of Cetirizine and Levocetirizine in tablets using Oxone

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HPLC method development, validation and its application to investigate in vitro effect of pioglitazone on the availability of H₁ receptor antagonists