HPLC method for the analysis of α-tocopherol from Myriophyllum alterniflorum

Delmail, David; Buzier, Rémy; Simon, Stéphane; Hourdin, Philippe; Botineau, Michel; Labrousse, Pascal

doi:10.1007/s10600-011-0033-z

Cited by 6 publications

(3 citation statements)

References 6 publications

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“…After 35 days, plant clones were acclimatized over 2 weeks in a new medium with a chemical composition identical to the Vienne River (i.e. oligotrophic medium), as described in Delmail et al (2011a). 450 clones were thus transferred into culture boxes containing 300 mL of oligotrophic medium (apportioned 10 explants per box).…”

Section: In Vitro Assays In Artificial Media Supplemented By Copper Omentioning

confidence: 99%

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Comparative in vitro/in situ approaches to three biomarker responses of Myriophyllum alterniflorum exposed to metal stress

et al. 2019

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Section: In Vitro Assays In Artificial Media Supplemented By Copper Omentioning

confidence: 99%

“…This molecule occurs naturally in alternate watermilfoil, and its level changes reflect an oxidative stress relative to ROS production. The α-tocopherol concentration was assayed as described in the HPLC protocol developed specifically for M. alterniflorum (Delmail et al, 2011a).…”

Section: Vitamin E (α-Tocopherol)mentioning

confidence: 99%

Comparative in vitro/in situ approaches to three biomarker responses of Myriophyllum alterniflorum exposed to metal stress

et al. 2019

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“…During recent ecotoxicological studies of heavy-metal pollution, M. alterniflorum was reported to produce high amounts of scavengers, α-tocopherol, carotenoids and proline; potentially preventing phospholid denaturation and loss of membrane permeability (Delmail, Buzier et al 2011;Delmail et al 2011aDelmail et al , 2011b. Moreover, vitamin E levels in the vegetative parts of water-milfoil are more than double those in other plant species commonly used in pharmaceutical and cosmetic applications (Delmail, Buzier et al 2011). This poorly known hydrophyte has never been evaluated for its antioxidant potential along with polyphenolic contents.…”

Section: Introductionmentioning

confidence: 99%

DNA damage protection, antioxidant and free-radical scavenging activities ofMyriophyllum alterniflorumDC (Haloragaceae) vegetative parts

Delmail¹,

Abasq²,

Courtel³

et al. 2013

Acta Botanica Gallica

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DNA damage induced by free radicals is associated with mutation-based health impairment and cancers. The protective effect of five different extracts from an aquatic macrophyte, the alternate water-milfoil Myriophyllum alterniflorum DC (Haloragaceae), is investigated on the DNA disruption mediated by hydroxyl (HO • ) and hydroperoxyl (HOO • ) radicals, and their antioxidant activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and O 2 •À . Indeed, M. alterniflorum is supposed to be a good candidate for DNA protection as this species was recently used in ecological surveys as a bioindicator of water quality because of its ability to cope with and to reflect the heavy-metal pollution through variations of antioxidant contents. Moreover, preliminary results indicated higher levels of scavengers specialized in reactive oxygen species reduction, than common medicinal plants. Acetone and ethanol extracts from vegetative parts limit significantly the oxidative damage of plasmid DNA induced by Fenton-reaction reactive oxygen species. The radical antioxidant activities of acetone and aqueous extracts are higher than those of other solvents:[DPPH] IC 50 = 2.4 ± 0.2 mg.ml -1 and [O 2•À ] AI 50 = 220.0 ± 14.1 μg.ml -1 , respectively. Protection towards free radicals is correlated with high contents of antioxidant compounds in acetone extracts [phenol compounds: 21.00 ± 0.69 mg gallic acid.g -1 DW (dry weight)] and aqueous extracts (flavonoids: 125.48 ± 1.26 mg rutin.g -1 DW). Myriophyllum alterniflorum can be regarded as a promising natural-product source of antigenotoxics and antioxidants and could be envisaged for therapeutic purposes.

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Halotolerance in Lichens: Symbiotic Coalition Against Salt Stress

Delmail

Grube

Parrot

et al. 2012

Ecophysiology and Responses of Plants Under Salt Stress

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International audienceLichens are among the most conspicuous and ubiquitous symbiosis on this planet. They are highly adapted to terrestrial habitats of all climatic zones including the most hostile environments on Earth, such as high altitudes in the Himalayas or the cold deserts of Antarctica. Among the extreme habitats are the littoral (or intertidal) zones of coasts. In this chapter, we present an overview of the current knowledge about the halotolerance mechanisms in lichens. Halotolerant organisms generally accumulate osmotically active solutes to cope with increasing external salinity. In intertidal lichens, mannitol could play an important role in osmoregulation. Epilichenic bacterial colonies may be also involved in limiting lichen nutrient imbalance by producing osmoprotective compounds and storing high ionic concentrations. In addition, the comparison with related inland species suggests that morphological adaptations could also be involved in adaptation to increased salt levels. Maritime species often have strongly conglutinated hyphae and small or no intercellular spaces in their thalli. So far, little genetic information exists about the genes involved in halotolerance and their regulation. Comparison of forthcoming genomic information from lichen fungi with those of other halotolerant fungi will soon help to change the picture and reveal genetic adaptations to saline environments

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HPLC method for the analysis of α-tocopherol from Myriophyllum alterniflorum

Abstract: Published in Khimiya Prirodnykh Soedinenii, No. 4, p. 594, July-August, 201

Cited by 6 publications

References 6 publications

Comparative in vitro/in situ approaches to three biomarker responses of Myriophyllum alterniflorum exposed to metal stress

Comparative in vitro/in situ approaches to three biomarker responses of Myriophyllum alterniflorum exposed to metal stress

DNA damage protection, antioxidant and free-radical scavenging activities ofMyriophyllum alterniflorumDC (Haloragaceae) vegetative parts

Halotolerance in Lichens: Symbiotic Coalition Against Salt Stress

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