HPLC Method for the Simultaneous Analysis of Fluoroquinolones and Oxazolidinones in Plasma

Abstract

A chromatographic method was implemented and validated for the simultaneous determination of antimicrobials proposed for the treatment of mycetoma: three fluoroquinolones: ciprofloxacin, moxifloxacin and sparfloxacin; two oxazolidinones: DA-7157 (DA2; torezolid) and its prodrug DA-7218 (DA1). Separation of analytes was achieved on an Atlantis dC18 column (150 × 4.6 mm i.d., 5 µm particle size) with a mobile phase composed of acetonitrile and trifluoroacetic acid 0.1% (v/v) using a gradient program. Total running time was 30 min. Quantification of sparfloxacin was carried out using a DAD at 278 nm; the oxazolidinones DA1 and DA2 and the quinolones ciprofloxacin and moxifloxacin were analyzed by fluorescence with an excitation wavelength of 292 nm and an emission wavelength of 525 nm. Intraday precision was in the range of 3.2 and 14.1%. Linearity range was from 0.3 to 10 µg/mL for sparfloxacin using DAD detector, and from 0.2 to 10 µg/mL for ciprofloxacin, 0.3 to 10 µg/mL for DA2, 0.4 to 10 µg/mL for DA1 and 0.04 to 10 µg/mL for moxifloxacin with fluorescence detector. Acetonitrile was used to precipitate proteins from plasma. Recoveries at low, medium and high concentration were between 80 and 120%. Limits of quantification were between 0.04 and 0.4 µg/mL in plasma. The method can be applied for individual or simultaneous determination of the antimicrobials in plasma.

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“…Moxifloxacin is chemically known as [1-Cyclopropyl-b-fluoro-1, 4-dihydro-8-methoxy-7-[(4aS, 7aS)-octahydro-6H-pyrrolo [3,[4][5][6] pyridine-6-yl]-4-oxo-3-quinoline carboxylic acid, is a fluoroquinolone broad-spectrum antibiotic agent and used in conjunctivitis. A few analytical techniques have been constructed for quantification of several drugs based on literature survey such as UV Spectrophotometry [33][34][35][36] RP-HPLC [37][38][39][40] , HPLC [41][42] , HPLC-MS [43][44] , LC-MS 45 , LC 46 , chemiluminescence 47 , Tanden mass spectrometry 48 methods for determination of Moxifloxacin have been reported.…”

Analytical Methods for Determining Third and Fourth Generation Fluoroquinolones: A Review

“…It was found that the tailing effect is reduced and the elution time of SPAR was 10-fold lower in UPLC analysis. The number of theoretical plates was three times greater for UPLC than for HPLC The detection applied in chromatographic analysis were UV [ 17 , 53 , 54 ], diode array detector (DAD) [ 57 ], and MS [ 35 , 55 ]. The MRM employed in MS/MS analysis were: m / z 393.2 → 349.3 [ 35 ] and m / z 392.9 → 348.7 [ 55 ].…”

“…The separation was performed on a C18 column. The analysed matrices via separation techniques were plasma, urine, serum, and tissues (muscle) [ 35 , 53 – 57 ]. The organic solvent applied in analysis was ACN [ 17 , 35 , 53 – 55 , 57 ].…”

Antimycobacterial Activities of N-Substituted-Glycinyl 1H-1,2,3-Triazolyl Oxazolidinones and Analytical Method Development and Validation for a Representative Compound

“…We hereby report the in vitro antimycobacterial activity of the previously reported N -substituted-glycinyl-1 H -1,2,3-triazolyl oxazolidinones and the development of a fast, reliable, and validated bio-analytical liquid chromatographic–mass spectrometric (LC-MS) method of analysis for estimating the plasma concentration of PH-189 in the presence of other biological constituents and to indicate any product(s) of instability. Literature survey showed that different instrumental methods have been reported for assessing analysis of structurally diverse oxazolidinone derivatives and their instability products in plasma [ 16 , 17 , 18 , 19 , 20 ].…”