HPLC‐MS/MS Analysis of <i>Aconiti Lateralis Radix Praeparata</i> and Its Combination with Red Ginseng Effect on Rat CYP450 Activities Using the Cocktail Approach

Ma, Wenxue; Wang, Wei; Huang, Xuhua; Yao, Guangzhe; Jia, Qi; Shen, Jiayuan; Ouyang, Hongwei; Chang, Yan‐xu; He, Jun

doi:10.1155/2020/8603934

Cited by 5 publications

(1 citation statement)

References 17 publications

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“…Cytochrome P450 (CYP450) is a super large gene family composed of a variety of isoenzymes involved in more than 90% of the body's drug metabolism [ 1 , 2 ]. CYP1, CYP2, and CYP3 gene families are the leading CYP families involved in the metabolism of most drugs in the body.…”

Section: Introductionmentioning

confidence: 99%

[Retracted] Compound Shuganjieyu Capsule on CYP450 Enzyme Activity

Tang

2022

BioMed Research International

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Objective. To evaluate the potential drug-drug interactions of the Shuganjieyu capsule by establishing a Chronic Unpredictable Mild Stress (CUMS) depression model combined with the Cocktail probe substrate method. Methods. The whole study was divided into the single-dose and multiple-dose groups. Animals were randomly divided into further subgroups in each group. Following chronic unpredictable mild stress model development, Shuganjieyu capsules were administered to the drug administration group. The single-dose subgroup received the drug for one day, and the multiple-dose subgroup received the drug for three months. Liver microsomes of each group were extracted, and the effects of the Shuganjieyu capsule on the CYP450 enzyme were investigated by liquid chromatography-mass spectrometry (LC-MS) (LC-MS) based on the Cocktail probe substrate method. The immunohistochemical method and RT-qPCR were used to detect the activity of CYP450 immunoprotein in rat liver. Results. In the single-dose subgroup, there were no statistical differences between the administration and model groups. In the multiple-dose subgroup, the conversion and protein expression rates of CYP1A2 and CYP2C19 were significantly increased in the model group compared with the blank group. In the administration group, the conversion and protein expression rates of CYP1A2 and CYP2C19 were inhibited. Conclusion. Long-term administration of the Shuganjieyu capsule could relieve depression-related behaviors in CUMS rats and downregulate the CYP1A2 and CYP2C19 enzyme activities in CUMS rats by inhibiting the expression of CYP1A2 and CYP2C19 protein. Long-term administration of the Shuganjieyu capsule may affect the bioavailability of other drugs metabolized by CYP1A2 and CYP2C19 enzymes, but the clinical guidance’s specific significance needs to be clarified further.

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Section: Introductionmentioning

confidence: 99%

[Retracted] Compound Shuganjieyu Capsule on CYP450 Enzyme Activity

Tang

2022

BioMed Research International

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Rapid multichannel fluorescent probe assay for CYP450 inhibition screening and drug interaction monitoring

Song,

Zeng,

Long

et al. 2024

Microchemical Journal

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Metabolic mechanism and pharmacological study of albendazole in secondary hepatic alveolar echinococcosis (HAE) model rats

Li,

zhang,

Pang

et al. 2024

Antimicrob Agents Chemother

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Albendazole (ABZ) is the primary treatment for alveolar echinococcosis (AE); however, its limited solubility impacts oral bioavailability, affecting therapeutic outcomes. In this study, various ABZ-solubilizing formulations, including albendazole crystal dispersion system (ABZ-CSD), albendazole hydrochloride-hydroxypropyl methylcellulose phthalate composite (T ABZ-HCl-H ), and albendazole hydroxyethyl sulfonate-hydroxypropyl methylcellulose phthalate composite (T ABZ-HES-H ), were developed and evaluated. Physicochemical properties as well as liver enzyme activity were analyzed and their pharmacodynamics in an anti-secondary hepatic alveolar echinococcosis (HAE) rat model were investigated. The formulations demonstrated improved solubility, exhibiting enhanced inhibitory effects on microcysts in HAE model rats compared to albendazole tablets. However, altered hepatic drug-metabolizing enzymes in HAE model rats led to increased ABZ levels and reduced ABZ-SO production, potentially elevating drug toxicity. These findings emphasize the importance of dose adjustments in patient administration, considering the impact of alveolar echinococcosis on rat hepatic drug metabolism.

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HPLC‐MS/MS Analysis of Aconiti Lateralis Radix Praeparata and Its Combination with Red Ginseng Effect on Rat CYP450 Activities Using the Cocktail Approach

Cited by 5 publications

References 17 publications

[Retracted] Compound Shuganjieyu Capsule on CYP450 Enzyme Activity

[Retracted] Compound Shuganjieyu Capsule on CYP450 Enzyme Activity

Rapid multichannel fluorescent probe assay for CYP450 inhibition screening and drug interaction monitoring

Metabolic mechanism and pharmacological study of albendazole in secondary hepatic alveolar echinococcosis (HAE) model rats

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