HPLC-MS<sup>(n)</sup> Applications in the Analysis of Anthocyanins in Fruits

Yuzuak, Seyit; Qian, Ma; Li, Yin; Xie, De‐Yu

doi:10.5772/intechopen.110466

Cited by 2 publications

References 107 publications

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Polyphenol exposure of mothers and infants assessed by LC–MS/MS based biomonitoring in breast milk

Berger,

Oesterle,

Ayeni

et al. 2024

Anal Bioanal Chem

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Exposure to polyphenols is relevant throughout critical windows of infant development, including the breastfeeding phase. However, the quantitative assessment of polyphenols in human breast milk has received limited attention so far, though polyphenols may positively influence infant health. Therefore, a targeted LC–MS/MS assay was developed to investigate 86 analytes representing different polyphenol classes in human breast milk. The sample preparation consisted of liquid extraction, salting out, freeze-out, and a dilution step. Overall, nearly 70% of the chemically diverse polyphenols fulfilled all strict validation criteria for full quantitative assessment. The remaining analytes did not fulfill all criteria at every concentration level, but can still provide useful semi-quantitative insights into nutritional and biomedical research questions. The limits of detection for all analyzed polyphenols were in the range of 0.0041–87 ng*mL−1, with a median of 0.17 ng*mL−1. Moreover, the mean recovery was determined to be 82% and the mean signal suppression and enhancement effect was 117%. The developed assay was applied in a proof-of-principle study to investigate polyphenols in breast milk samples provided by twelve Nigerian mothers at three distinct time points post-delivery. In total, 50 polyphenol analytes were detected with almost half being phenolic acids. Phase II metabolites, including genistein-7-β-D-glucuronide, genistein-7-sulfate, and daidzein-7-β-D-glucuronide, were also detected in several samples. In conclusion, the developed method was demonstrated to be fit-for-purpose to simultaneously (semi-) quantify a wide variety of polyphenols in breast milk. It also demonstrated that various polyphenols including their biotransformation products were present in breast milk and therefore likely transferred to infants where they might impact microbiome development and infant health. Graphical abstract

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Polyphenol exposure of mothers and infants assessed by LC–MS/MS based biomonitoring in breast milk

Berger,

Oesterle,

Ayeni

et al. 2024

Anal Bioanal Chem

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Perspectives of Organic Dyes Cosensitization and Its Utilization in TiO₂ Nanoclusters for Photocatalysis Applications

Barraza-Jiménez,

Manuel Lerma Mancinas,

Iván Flores-Hidalgo

et al. 2024

Nanotechnology and Nanomaterials

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Cosensitization has emerged as a method to improve performance of dye sensitized solar cells (DSSCs) and photocatalysis. In this work, it is proposed to use organic dyes as cosensitizers due to their friendliness with the environment and to the benefits of having two or more different dyes with complementary optical absorption characteristics. Several organic dyes are analyzed as cosensitizers to identify which dye combinations may be good choices to approach a panchromatic absorption spectrum emulating the solar emission spectrum. In addition to the analysis on the prospective sensitizers, it is presented results of titanium dioxide (TiO2) nanoclusters cosensitized with two anthocyanidins using density functional theory (DFT) and time-dependent DFT (TD-DFT). The nanocluster size proved to be definitive in the interactions with two molecule dyes. The selected (TiO2)4–5 nanoclusters cosensitized with two anthocyanidins produce data for a prospective analysis to suggest which dyes are good options for DSSCs and photocatalysis based on dye co-sensitization applications. At the end, one can look at this work as a perspective of which organic dyes may work well as cosensitizers and a contrast to original data from our experimentation with a couple of TiO2 nanoclusters cosensitized with two different anthocyanidins.

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HPLC-MS⁽ⁿ⁾ Applications in the Analysis of Anthocyanins in Fruits

Cited by 2 publications

References 107 publications

Polyphenol exposure of mothers and infants assessed by LC–MS/MS based biomonitoring in breast milk

Polyphenol exposure of mothers and infants assessed by LC–MS/MS based biomonitoring in breast milk

Perspectives of Organic Dyes Cosensitization and Its Utilization in TiO₂ Nanoclusters for Photocatalysis Applications

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HPLC-MS(n) Applications in the Analysis of Anthocyanins in Fruits

Cited by 2 publications

References 107 publications

Polyphenol exposure of mothers and infants assessed by LC–MS/MS based biomonitoring in breast milk

Polyphenol exposure of mothers and infants assessed by LC–MS/MS based biomonitoring in breast milk

Perspectives of Organic Dyes Cosensitization and Its Utilization in TiO2 Nanoclusters for Photocatalysis Applications

Contact Info

Product

Resources

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HPLC-MS⁽ⁿ⁾ Applications in the Analysis of Anthocyanins in Fruits

Perspectives of Organic Dyes Cosensitization and Its Utilization in TiO₂ Nanoclusters for Photocatalysis Applications