HPLC-PDA analysis of ACE-inhibitors, hydrochlorothiazide and indapamide utilizing design of experiments

Abstract

A simple, rapid, precise, accurate and sensitive high performance liquid chromatographic method has been developed for simultaneous determination of ACE inhibitors with hydrochlorothiazide and indapamide in pharmaceutical formulations. 'Design of Experiments' (DoE) using 'central composite design' (CCD) was applied to facilitate method development and optimization. Mobile phase was optimized utilizing response surface methodology using Design Expert software. Chromatographic separation was achieved on HypersilÒ-Gold C 18 (100 · 4.6 mm, 3 lm, Thermo Fisher Scientific, USA), column at 25°C. The mobile phase was 58% buffer (5 mM KH 2 PO 4 , containing triethylamine 0.25 ml/L), 25% acetonitrile and 17% methanol (pH adjusted to 2.8 ± 0.1). The analysis was performed at 215 nm. The mobile phase flow rate was 1.0 ml/min and injection volume 10 ll. The method was validated for linearity, limits of quantitation and detection, accuracy, precision, ruggedness and robustness as per the International Conference on Harmonization (ICH) guidelines. Calibration curves (for lisinopril, hydrochlorothiazide, captopril, imidapril, perindopril, indapamide and trandolapril) were linear in the concentration range of 5-35 lg/ml. The limit of detection and limit of quantitation for experimental drugs ranged from 0.03 to 0.61 and 0.08-1.84 lg/ml respectively. ª 2014 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

Switch on fluorescence probe for the selective determination of lisinopril in pharmaceutical formulations: application to content uniformity testing

“…The oral dosage of lisinopril ranges from 5 to 40 mg daily (single or divided dosage), with 5 and 10 mg daily being appropriate for the initiation of therapy for heart failure and hypertension, respectively. 1 Different analytical methods have been developed for determination of lisinopril including; spectrophotometric 2-10 spectrouorimetric, [10][11][12][13][14][15] polarographic, 7 chromatographic, 4,9,[16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] capillary electrophoretic [32][33][34][35] ow injection 36 and immunoassay 37 methods.…”

“…The chromatographic and electrophoretic methods require expensive instruments and consume a large volume of expensive organic solvents. 4,[17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] In addition, the spectrophotometric methods are less sensitive. [2][3][4][5][6][7][8][9][10] On the other hand spectro-uorimetry is a simple and inexpensive technique although it has high sensitivity, selectivity and reliable accuracy.…”

Optimization of HPLC Using Central Composite Design for Determination of Curcumin and Demethoxycurcumin in Tablet Dosage Form

“…CCD was selected to give complete response under extreme conditions (highest or lowest levels of independent variables) which is not possible obtained using Box-Behnken design. 18 CCD was carried out using Design Expert (Version 10 (Trial Version), Stat-Ease Inc., Minneapolis, MN USA). Thirty experiments were constructed using four variables (X1, X2, X3 and X4) along with observed responses as described in table 1.…”

Two smart spectrophotometric methods for simultaneous determination of Lisinopril and Hydrochlorothiazide in binary mixtures

“…LIS was previously analyzed by titrimetric [4], spectrophotometric [5][6][7][8][9][10][11][12][13][14], Spectrofluorimetric [15][16][17][18][19], chromatographic [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38], electrophoretic [39,40] and electrochemical methods [4,41,42]. Mixtures of LIS and HCT were analyzed simultaneously by spectrophotometric [43][44][45], chromatographic [21,22,29,[46][47][48], electrophoretic [39] and electrochemical methods …”