HPLC‐QTOF‐MS/MS profiling, antioxidant, andα‐glucosidase inhibitory activities ofPyracantha fortuneanafruit extracts

Abstract

This study was carried out to optimize the solvent for extracting the antioxidants and α‐glucosidase inhibitors (AGIs) from Pyracantha fortuneana fruit (PFF) and the major chemical components were characterized by HPLC‐QTOF‐MS/MS. The results showed that 50% and 70% acetone (v/v, ml/ml) gave the best extraction efficiency on phenolics and total flavonoids, while 70% acetone and 50% methanol possess better recovery on protein and polysaccharides, respectively. In addition, the 50% and 70% acetone extracts gave the strongest radical scavenging ability and α‐glucosidase inhibitory activity (p > 0.05), but the Fe3+ reducing power of the 50% acetone extract was higher than that of 70% acetone. Correlation analysis indicated that phenolic acids and flavonoids were connected to the antioxidant activity and α‐glucosidase inhibitory activity closely. Moreover, 25 compounds including 7 flavonoids, 6 phenolic acids, 7 organic acids, 3 tannins, 1 terpene, and 1 alkaloid were identified or tentatively identified in the 50% acetone extract. Overall, 50% acetone can be a proper solvent for extracting antioxidants and AGIs from PFF. Practical applications Imbalance between production and clearance of reactive oxygen species (ROS) in the body could induce various chronic diseases. PFF is an edible fruit beneficial to human health; it is reported to be capable of optimizing blood glucose levels and may prevent premature aging. In the present study, PFF was found to be excellent in antioxidant activities and α‐glucosidase inhibitory ability; 50% acetone was found to be the best extraction solvent. In addition, the predominant phytochemical components of the 50% acetone extract were characterized. This study can promote further research of Pyracantha fortuneana in natural functional products, especially in the prevention of type II diabetes and its complication.

Effects of different drying methods on the physicochemical property and edible quality of fermented Pyracantha fortuneana fruit powder

“…Li, a wild evergreen shrub or small arbour belonging to the Pyracantha of Osaceae apple subfamily, is mainly distributed in areas from eastern Asia to southern Europe. Pyracantha fortuneana fruit (PFF), as a new food resource, has become a research hotspot due to its rich nutrients and natural active ingredients, and it is even treated as a staple food in some areas in case of food shortage (Wei et al ., 2017; Wang et al ., 2019). However, most PFF are wild fruits, with several disadvantages for eating, such as small fruit size, rough and astringent taste, fresh fruit seasonality and intolerance to storage.…”

Enrichment of flavonoid‐rich extract from Bidens bipinnata L. by macroporous resin using response surface methodology, UHPLC–Q‐TOF MS/MS‐assisted characterization and comprehensive evaluation of its bioactivities by analytical hierarchy process

“…The total flavonoid extract of Nelumbo nucifera leaf showed inhibitory activity against α ‐amylase and α ‐glucosidase with IC 50 values of 2.20 and 1.86 mg/ml (Liu et al, 2013). The 50 and 70% acetone extracts of Pyracantha fortuneana fruit with higher phenolic and flavonoid contents showed higher α ‐glucosidase inhibitory activity than methanol and ethanol extracts (Wang et al, 2018). The results of our study seemed to agree with these findings.…”

Biological activities and health-promoting effects of Pyracantha genus: a key approach to the phytochemical's potential

“…Several studies showed the presence of phytochemicals, like glycosides, carbohydrates, steroids, flavonoids, phenolics, resins and tannins in Pyracantha spp. (18,(29)(30)(31). For example, Fico, A (32) found phenolic acids, such as hyperoside, isoquercitrin, quercetin and rutin in P. coccinea fruits.…”

“…Wang, Ye (31) with the help of high-pressure liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS/MS) profiling identified 7 flavonoids, 6 phenolic acids, 7 organic acids, 3 tannins, 1 terpene, and 1 alkaloid from P. fortuneana fruit (50% acetone extract) and reported antioxidant and α-glucosidase inhibitory activities. Wei, Chai (50) extracted proanthocyanidins from P. fortuneana fruit and reported α-glucosidase inhibition (IC 50 0.15 µg/mL) in a non-competitive manner.…”