HPLC–RIA analysis of steroid hormone profile in a virilizing stromal tumor of the ovary

Szécsi, Mihály; Tóth, István; Gardi, János; Nyári, Tibor; Julesz, J.

doi:10.1016/j.jbbm.2004.04.008

Cited by 8 publications

(3 citation statements)

References 35 publications

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“…It seems evident that the impact of androgens on women's health has been underestimated. Elevated levels of steroids in the peripheral serum have also been found in patients measured under the routine diagnostic assay before the tumor resection [38]. Here, the ovarian tumor samples were obtained through unilateral oophorectomy.…”

Section: Plasma/serum Estrogen and Androgen In Breast Cancer Risksmentioning

confidence: 99%

Plasma Steroid Level Measured Using Modern Separation Techniques as Biomarkers in Biological Diagnostics

Konieczna¹,

Bączek

2010

CPA

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During the last decade there has been an increased focus on the application of steroids as biomarkers in biomedical practice. The analysis of steroid hormones in biological samples of plasma or serum is currently routinely used in clinical diagnosis being an essential source of information on not only metabolic pathways, but also disorders of the metabolism. More importantly, the steroid hormones may reduce cancer development on the endocrinal basis, reveal abuse of anabolic substances, or even depression incidences. In the case of biomedical research, quantitative determination of steroids in serum or plasma creates an opportunity to diagnose diseases efficiently in their early stages and to monitor a patient for a possible recurrence of a disease after therapy. Therefore, an accurate measurement of steroids in the plasma or serum has become important for the contemporary medicine, even if troublesome especially in view of their low concentration in biological samples.The most common methods of steroid quantification in clinical practice nowadays include immunoassays such as radioimmunoassay or enzyme immunoassay. However, the main disadvantage of the immunoassay techniques consists in cross reactivity of the antibodies used in the assay with the related hormones. On the other hand, there is a number of applications where the analysis is performed with the use of modern separation techniques.The aim of this review is to present the development and applicability of the various analytical methods, all based on separation techniques and used for determination of steroids in the clinical laboratory, taking into account the recent progress in those areas. The review shows the advantages of high-performance liquid chromatography applied to determine non-volatile and thermally labile steroids and steroid conjugates. It also demonstrates that the currently used mass spectrometry offers practically useful structural information on the composition of steroids. A number of techniques and methods are compared and critically discussed pondering finally their specific analytical requirements like sensitivity, specificity, simplicity, limit of detection, and quantification. Nonetheless, plasma or serum sampling seems to remain one of the most convenient methods for population screening purposes.

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Section: Plasma/serum Estrogen and Androgen In Breast Cancer Risksmentioning

confidence: 99%

Plasma Steroid Level Measured Using Modern Separation Techniques as Biomarkers in Biological Diagnostics

Konieczna¹,

Bączek

2010

CPA

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“…By considering the importance of the steroid and hormone families, numerous attempts have been made through the years in order to detect them with high sensitivity using different analytical techniques. Most of the biological samples containing steroids and hormones are analyzed using gas or liquid chromatography coupled with the mass spectrometer [17][18][19][20][21][22]. Determination of the analyte by using these techniques is very accurate; however, the whole analytical procedure is expensive, time-consuming and often requires long and complicated sample pretreatment and specialized skills of the analyst.…”

Section: Introductionmentioning

confidence: 99%

Advances on Hormones and Steroids Determination: A Review of Voltammetric Methods since 2000

Smajdor

Paczosa‐Bator²,

Piech³

2022

Membranes

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This article presents advances in the electrochemical determination of hormones and steroids since 2000. A wide spectrum of techniques and working electrodes have been involved in the reported measurements in order to obtain the lowest possible limits of detection. The voltammetric and polarographic techniques, due to their sensitivity and easiness, could be used as alternatives to other, more complicated, analytical assays. Still, growing interest in designing a new construction of the working electrodes enables us to prepare new measurement procedures and obtain lower limits of detection. A brief description of the measured compounds has been presented, along with a comparison of the obtained results.

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“…Of these techniques, only mass spectrometry provides structural information, but with higher cost and poorer sensitivity (pmol) relative to that of the immunoassaybased analyses (fmol) [6].…”

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confidence: 99%

Determining estrogens using surface-assisted laser desorption/ionization mass spectrometry with silver nanoparticles as the matrix

Chiu

Chang

Chiang

et al. 2008

J. Am. Soc. Mass Spectrom.

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We describe the application of silver nanoparticles (Ag NPs) as matrices for the determination of three estrogens using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Because Ag NPs have extremely high absorption coefficients (1.2 x 10(8) M(-1) cm(-1)) at 337 nm, they are effective SALDI matrices when using a nitrogen laser. Three tested estrogens--estrone (E1), estradiol (E2), and estriol (E3)--adsorb weakly onto the surfaces of the Ag NPs, through van der Waals forces. After centrifugation, the concentrated analytes adsorbed on the Ag NPs were subjected directly to SALDI-MS analyses, with the limits of detection for E1, E2, and E3 being 2.23, 0.23, and 2.11 microM, respectively. The shot-to-shot and batch-to-batch variations for the three analytes were less than 9% and 13%, respectively. We validated the practicality of this present approach through the quantitation of E2 in human urine. Using this approach, we determined the concentration of E2 in a sample of a pregnant woman's urine to be 0.16+/-0.05 microM (n=10).

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HPLC–RIA analysis of steroid hormone profile in a virilizing stromal tumor of the ovary

Cited by 8 publications

References 35 publications

Plasma Steroid Level Measured Using Modern Separation Techniques as Biomarkers in Biological Diagnostics

Plasma Steroid Level Measured Using Modern Separation Techniques as Biomarkers in Biological Diagnostics

Advances on Hormones and Steroids Determination: A Review of Voltammetric Methods since 2000

Determining estrogens using surface-assisted laser desorption/ionization mass spectrometry with silver nanoparticles as the matrix

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