HPLC separation of phenolic phytochemicals from grape peels and seeds water extracts and their in-vitro antimalarial activities

Akkawi, Mutaz; Abu‐Lafi, Saleh; Abu-Remeleh, Qassem; Kiswani, Marah; Qutob, Mutaz; Nusseibeh, Sameh; Lutgen, Pierre

doi:10.15406/ppij.2018.06.00183

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…The need for such drugs is crucial due to the emergence of drug-resistant strains of Plasmodium, including resistance to widely used drugs like chloroquine. 15,16 Moreover, the improper use of antimalarials has contributed to common malaria parasite resistance to many drugs on the market. 17 Throughout history, plant-based medications have been utilized for medical purposes, and many pharmaceutical therapies still use them today.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Analysis of Antimalarial Activity of Achillea fragrantissima (Forssk.) Sch.Bip Extracts Based on Beta-Hematin Formation

Al-Rimawi,

Akkawi,

Tarayrah

et al. 2024

Natural Product Communications

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Introduction: The use of medicinal plants from traditional knowledge systems has increased in recent years. Achillea fragrantissima-infused water has a historical use in relieving gastrointestinal complaints and treating skin inflammations. With the rising resistance to antimalarial drugs, finding safe and affordable new drugs is crucial. Exploring medicinal plants shows promise for drug development in the fight against malaria. Methods: The objective of this research is to examine the in vitro antimalarial activity of A. fragrantissima-infused water, as well as water-ethanol extracts of its leaves, flowers, and stems based on β-hematin formation. Colorimetric semiquantitative assessments based on β-hematin inhibition and evaluation of optical density at 405 nm were employed. Results: The results demonstrated promising antimalarial activity of the different plant parts. The aqueous flower extract exhibited strong activity, comparable to that of the positive control. The leaves extract also showed strong antimalarial activity. The stem's aqueous and ethanolic extracts exhibited lower inhibitory activity compared to flowers and leaves. Synergistic antimalarial activity was observed when mixing the stem and leaves water extracts, as well as when mixing each of the stem and leaves extracts with the flower extract. The highest synergistic effect was observed when all three parts of the plant were mixed, resulting in activity comparable to the positive control. The high-performance liquid chromatography photodiode array (HPLC-PDA) chromatographic method confirmed the presence of rutin in the leaves extract. Conclusion: The findings of this study highlight the potential antimalarial activity of A. fragrantissima. The aqueous flower extract and leaves extract exhibited strong antimalarial activity, while the stem extracts showed relatively lower activity. Furthermore, synergistic effects were observed when combining different parts of the plant. These results suggest that A. fragrantissima holds promise as a potential source for the development of new antimalarial drugs. The confirmation of rutin presence in the leaves extract through HPLC-PDA analysis provides additional insights into the plant's chemical composition.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Analysis of Antimalarial Activity of Achillea fragrantissima (Forssk.) Sch.Bip Extracts Based on Beta-Hematin Formation

Al-Rimawi,

Akkawi,

Tarayrah

et al. 2024

Natural Product Communications

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show abstract

“…9,10 Therefore, a candidate for an effective antimalarial drug works by preventing the formation of hemozoin, which eliminates the parasite. [11][12][13][14][15] There are several effective treatments for malaria, including medications such as chloroquine, and artemisinin-based combination therapies (ACTs), etc. Recent years have seen an increase in the resistance of malaria parasites to widely used drugs.…”

Section: Introductionmentioning

confidence: 99%

“…Utilizing the potential of natural polyphenols may provide creative approaches to the ongoing fight against malaria. [11][12][13][14][15] Identification of key components in water extracts of tea (Camellia Sinensis) using UPLC-PDA-ESi-MS and evaluation of their possible antimalarial properties Tea (Camellia Sinensis) belongs to Theaceae family and is considered one of the most consumed beverages worldwide. It is one of those rare beverages that are universally accepted by science for their numerous health benefits.…”

Section: Introductionmentioning

confidence: 99%

Identification of key components in water extracts of tea (Camellia Sinensis) using UPLC-PDA-ESiMS and evaluation of their possible antimalarial properties

Abu-Lafi,

Akkawi,

Abu-Remeleh

2023

PPIJ

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This study used a semi-quantitative approach to examine how water extracted green and black tea (Camellia Sinensis) inhibits the formation of β-hematin in-vitro. LC-PDA-ESi-MS analysis of the tea extracts revealed that they contain many active catechins. When compared to the positive control, chloroquine, the results demonstrated that black water extracts had a considerable anti-malarial potential even at significantly lower dosages, while the green tea water extracts showed no effect. However, when a bicarbonate solution was used to create the green tea infusion, the result was similarly effective to the positive control. Additionally, there was no difference in β-hematin formation when the black tea was prepared at various time points (freshly prepared vs. 24-48 hours of incubation). The absence of time-dependent effects suggests that the in-vitro assay is unaffected by oxidation or any other changes that may have occurred in the extracts and solely reacts to stable active compounds or minerals that prevent β-hematin formation. The diverse combined catechins present in the tea extracts were efficient in forming a favorable complex with free heme, thereby preventing β-hematin formation, which could irreversibly harm the plasmodium parasite during the intraerythrocytic stage.

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Alcohol and malaria

Lutgen¹

2018

PPIJ

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tropical countries. 1,2 During gametocytogenenesis malaria parasites hide in the bone marrow. Ethanol has an effect on bone marrow. Biopsies from 30 alcohol-dependent individuals were investigated. The findings took the form of heightened ineffective erythropoiesis in bone marrow associated with impaired iron utilization. Both may be detrimental to the survival of the gametocytes. 3 The effect of ethanol on the in vitro growth of the malaria parasite Plasmodium falciparum was investigated during six days of incubation. A significant growth inhibition for ethanol concentrations was observed on each day. Malarial parasites are strongly inhibited by ethanol concentrations. 4,5 Fever is accompanied by glycogen destruction. This was already discovered more than 100 years ago. Glycogen disappears from the liver during tetanus, diphtheria and pneumonia. A natural way of our body to fight parasites and diseases. It makes thus complete nonsense to fight fever in the early stages of a malaria infection. 6,7 Alcohol also removes glycogen from the liver. 8 Chronic ethanol consumption also results in a dramatic decrease in liver glycogen concentrations, which could be related to either a depressed rate of synthesis or an increased rate of breakdown. 9 Macrophages, including Kupffer cells, appear to increase their production of cytokines in patients with alcoholic liver disease. Precursors of macrophages, i.e. monocytes with alcohol induced hepatitis produce greater amounts of TNF-α and reactive oxygen species. These data have been confirmed in animals. Malaria parasites are destroyed by oxidative species, like NO, H 2 O 2 or artemisinin peroxides. [10][11][12] Wine is efficient against other pathogens. An in vitro study was undertaken to determine the potential for survival of enteric pathogens in common drinking beverages. Three carbonated soft drinks, two alcoholic beverages, skim milk, and water were inoculated with Salmonella, Shigella, and enterotoxigenic Escherichia coli and quantitative counts were performed over 2 days. The study showed poorest survival of all three organisms in wine, and greatest growth in milk and water.

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HPLC separation of phenolic phytochemicals from grape peels and seeds water extracts and their in-vitro antimalarial activities

Cited by 7 publications

References 17 publications

In Vitro Analysis of Antimalarial Activity of Achillea fragrantissima (Forssk.) Sch.Bip Extracts Based on Beta-Hematin Formation

In Vitro Analysis of Antimalarial Activity of Achillea fragrantissima (Forssk.) Sch.Bip Extracts Based on Beta-Hematin Formation

Identification of key components in water extracts of tea (Camellia Sinensis) using UPLC-PDA-ESiMS and evaluation of their possible antimalarial properties

Alcohol and malaria

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