2021
DOI: 10.3390/molecules26102903
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HPLC-UV and GC-MS Methods for Determination of Chlorambucil and Valproic Acid in Plasma for Further Exploring a New Combined Therapy of Chronic Lymphocytic Leukemia

Abstract: High performance liquid chromatography with ultra-violet detection (HPLC-UV) and gas chromatography–mass spectrometry (GC-MS) methods were developed and validated for the determination of chlorambucil (CLB) and valproic acid (VPA) in plasma, as a part of experiments on their anticancer activity in chronic lymphocytic leukemia (CLL). CLB was extracted from 250 µL of plasma with methanol, using simple protein precipitation and filtration. Chromatography was carried out on a LiChrospher 100 RP-18 end-capped colum… Show more

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Cited by 11 publications
(8 citation statements)
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“…Detection of VAN and MER in human plasma with an LLOQ of 1 μg/mL, extracted by protein precipitation, was reported by Barco et al [ 12 , 13 ]. Lipska et al achieved a much lower LLOQ of 0.075 μg/mL for VPA in plasma using high-performance liquid chromatography with ultra-violet detection (HPLC-UV) and gas chromatography–mass spectrometry (GC-MS) methods [ 14 ]. Ye et al developed a high-throughput LC–MS/MS approach to measuring VAN in human CSF with an LLOQ of 0.1 μg/mL [ 15 ].…”
Section: Introductionmentioning
confidence: 99%
“…Detection of VAN and MER in human plasma with an LLOQ of 1 μg/mL, extracted by protein precipitation, was reported by Barco et al [ 12 , 13 ]. Lipska et al achieved a much lower LLOQ of 0.075 μg/mL for VPA in plasma using high-performance liquid chromatography with ultra-violet detection (HPLC-UV) and gas chromatography–mass spectrometry (GC-MS) methods [ 14 ]. Ye et al developed a high-throughput LC–MS/MS approach to measuring VAN in human CSF with an LLOQ of 0.1 μg/mL [ 15 ].…”
Section: Introductionmentioning
confidence: 99%
“…Valproic acid alleviates central nervous system (CNS) injuries and improves functional results after acute CNS damage by multiple pathways, including anti-inflammatory, anti-apoptotic, and neurotrophic effects [2]. Recent reports indicate that valproic acid can be used in the treatment of breast cancer [3] and in chronic lymphocytic leukemia [4]. Analytical methods are applied to determine the drug in human body fluids and in pharmaceutical preparations.…”
Section: Introductionmentioning
confidence: 99%
“…They are useful in monitoring the therapeutic concentration of a substance, treatment progress, and side effects. Several methods for determination of valproic acid are known, namely: thin layer chromatography [5], liquid chromatography [6][7][8][9], high performance liquid chromatography [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24], ultra high performance liquid chromatography [4,25,26], ultra performance liquid chromatography [27][28][29][30][31], gas chromatography [4,18,[32][33][34][35][36][37][38], capillary electrophoresis [39,40], voltamperometry [41], valproateselective electrode [42], and colorimetry [43].…”
Section: Introductionmentioning
confidence: 99%
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“…Consequently, the therapeutical drug monitoring (TDM) in patient's plasma is of crucial importance. From the analytical point of view, many methods for VPA determination in plasma exist in literature, and beside immunometric methods commonly used in clinical settings, high‐performance liquid chromatography (HPLC‐UV) 4 and gas chromatography–mass spectrometry (GC–MS) 5 have been gaining importance in recent years. Moreover, liquid chromatography coupled to mass spectrometry (LC–MS/MS) 6 , 7 , 8 has gained importance in TDM method development for its features of sensitivity, selectivity, and rapidity.…”
Section: Introductionmentioning
confidence: 99%